<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2025 Workiva-->
<!--r:217639f3-3601-4c34-a4f5-697bd18227a7,g:91c171eb-1d41-4a86-a68a-085fd4f464cb-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.biontech.de/role/Cover" xlink:type="simple" xlink:href="bntx-20241231.xsd#Cover"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/Cover" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityListingsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityListingsTable_ca3b4dec-63ce-461c-939e-5dc5b3102aaa" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityListingsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityListingsTable_ca3b4dec-63ce-461c-939e-5dc5b3102aaa" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressesAddressTypeAxis_6fbbbd9f-328f-4da4-9520-bf56d8481863" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressesAddressTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_EntityListingsTable_ca3b4dec-63ce-461c-939e-5dc5b3102aaa" xlink:to="loc_dei_EntityAddressesAddressTypeAxis_6fbbbd9f-328f-4da4-9520-bf56d8481863" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AddressTypeDomain_6fbbbd9f-328f-4da4-9520-bf56d8481863_default" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_AddressTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_EntityAddressesAddressTypeAxis_6fbbbd9f-328f-4da4-9520-bf56d8481863" xlink:to="loc_dei_AddressTypeDomain_6fbbbd9f-328f-4da4-9520-bf56d8481863_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AddressTypeDomain_707fd447-e510-456e-957a-d5739df286ec" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_AddressTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_EntityAddressesAddressTypeAxis_6fbbbd9f-328f-4da4-9520-bf56d8481863" xlink:to="loc_dei_AddressTypeDomain_707fd447-e510-456e-957a-d5739df286ec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_BusinessContactMember_b69e0664-a1e2-4de8-90d1-8b78c777e5f1" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_BusinessContactMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_AddressTypeDomain_707fd447-e510-456e-957a-d5739df286ec" xlink:to="loc_dei_BusinessContactMember_b69e0664-a1e2-4de8-90d1-8b78c777e5f1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalAxis_63f2fa6e-d493-4a2a-abb9-1886625aee41" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfShareCapitalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_EntityListingsTable_ca3b4dec-63ce-461c-939e-5dc5b3102aaa" xlink:to="loc_ifrs-full_ClassesOfShareCapitalAxis_63f2fa6e-d493-4a2a-abb9-1886625aee41" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalDomain_63f2fa6e-d493-4a2a-abb9-1886625aee41_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfShareCapitalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfShareCapitalAxis_63f2fa6e-d493-4a2a-abb9-1886625aee41" xlink:to="loc_ifrs-full_ClassesOfShareCapitalDomain_63f2fa6e-d493-4a2a-abb9-1886625aee41_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalDomain_50f966f0-496c-4bbe-8bc5-02d0e975d837" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfShareCapitalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfShareCapitalAxis_63f2fa6e-d493-4a2a-abb9-1886625aee41" xlink:to="loc_ifrs-full_ClassesOfShareCapitalDomain_50f966f0-496c-4bbe-8bc5-02d0e975d837" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_AmericanDepositarySharesEachRepresentingOneOrdinaryShareMember_de360f24-8e7a-4ffe-b97b-336796dcc0d9" xlink:href="bntx-20241231.xsd#bntx_AmericanDepositarySharesEachRepresentingOneOrdinaryShareMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfShareCapitalDomain_50f966f0-496c-4bbe-8bc5-02d0e975d837" xlink:to="loc_bntx_AmericanDepositarySharesEachRepresentingOneOrdinaryShareMember_de360f24-8e7a-4ffe-b97b-336796dcc0d9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OrdinarySharesNoParValueWithNationalAmountAttributableToEachOrdinaryShareMember_e781216d-2c54-4ec3-8cf8-6f84ea7923dd" xlink:href="bntx-20241231.xsd#bntx_OrdinarySharesNoParValueWithNationalAmountAttributableToEachOrdinaryShareMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfShareCapitalDomain_50f966f0-496c-4bbe-8bc5-02d0e975d837" xlink:to="loc_bntx_OrdinarySharesNoParValueWithNationalAmountAttributableToEachOrdinaryShareMember_e781216d-2c54-4ec3-8cf8-6f84ea7923dd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_a0bc5cb6-7244-4582-841b-d1693db36d7e" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_DocumentType_a0bc5cb6-7244-4582-841b-d1693db36d7e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentRegistrationStatement_acbb9c4f-b099-4615-bf85-1de275597949" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentRegistrationStatement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_DocumentRegistrationStatement_acbb9c4f-b099-4615-bf85-1de275597949" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentAnnualReport_ecf0c9dc-2dff-4c6b-bb72-b6801573c5cd" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentAnnualReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_DocumentAnnualReport_ecf0c9dc-2dff-4c6b-bb72-b6801573c5cd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_03701836-cafe-4d78-87da-bc30e11885ae" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentPeriodEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_DocumentPeriodEndDate_03701836-cafe-4d78-87da-bc30e11885ae" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CurrentFiscalYearEndDate_85e9a2dd-ad62-4fd6-b028-6bc8ffe72c82" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CurrentFiscalYearEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_CurrentFiscalYearEndDate_85e9a2dd-ad62-4fd6-b028-6bc8ffe72c82" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentTransitionReport_2fdb42d2-5513-4faf-97ed-d021d1d7fb8b" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentTransitionReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_DocumentTransitionReport_2fdb42d2-5513-4faf-97ed-d021d1d7fb8b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentShellCompanyReport_eb6026ce-e242-42ea-acfb-daea3a9fe865" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentShellCompanyReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_DocumentShellCompanyReport_eb6026ce-e242-42ea-acfb-daea3a9fe865" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_896b099d-cf5f-402d-af6e-c062648dadc0" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityFileNumber_896b099d-cf5f-402d-af6e-c062648dadc0" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_18f5956f-cf5a-4340-a0a6-e62c7b024419" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityRegistrantName_18f5956f-cf5a-4340-a0a6-e62c7b024419" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_045b4e60-b87f-4211-a18e-c307b24f311a" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityIncorporationStateCountryCode_045b4e60-b87f-4211-a18e-c307b24f311a" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_c6935c24-d514-48fc-8918-1bb016c1fb6e" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityAddressAddressLine1_c6935c24-d514-48fc-8918-1bb016c1fb6e" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_96fe8568-3154-400b-a8f4-538a1dba4bdc" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityAddressPostalZipCode_96fe8568-3154-400b-a8f4-538a1dba4bdc" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_a51f86a8-1238-4834-8f0f-88400fbfe057" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityAddressCityOrTown_a51f86a8-1238-4834-8f0f-88400fbfe057" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCountry_eebc9812-0ee6-43c3-bb32-4186c696b692" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressCountry"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityAddressCountry_eebc9812-0ee6-43c3-bb32-4186c696b692" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_ContactPersonnelName_23640ab5-750d-4f57-a097-185342d2fde0" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_ContactPersonnelName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_ContactPersonnelName_23640ab5-750d-4f57-a097-185342d2fde0" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_745bca4e-5b7b-4022-b991-01b9e5693cd2" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_CityAreaCode_745bca4e-5b7b-4022-b991-01b9e5693cd2" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_ad3dd7ce-0ba8-49d2-b623-ad9ce075ca33" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_LocalPhoneNumber_ad3dd7ce-0ba8-49d2-b623-ad9ce075ca33" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_ContactPersonnelFaxNumber_b1cc0c8c-ebfb-4bb1-b41c-63aa5fbc4424" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_ContactPersonnelFaxNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_ContactPersonnelFaxNumber_b1cc0c8c-ebfb-4bb1-b41c-63aa5fbc4424" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_ContactPersonnelEmailAddress_22af0913-185c-4544-8a48-d941fb79dff9" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_ContactPersonnelEmailAddress"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_ContactPersonnelEmailAddress_22af0913-185c-4544-8a48-d941fb79dff9" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_b64f0909-4f4f-4d55-b3c9-7699043c75ba" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_Security12bTitle_b64f0909-4f4f-4d55-b3c9-7699043c75ba" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_2b81d26b-b0a7-4b95-b0fd-ee38d82ee392" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_TradingSymbol_2b81d26b-b0a7-4b95-b0fd-ee38d82ee392" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_d50b8d18-196a-418b-b34d-e000825e853f" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_SecurityExchangeName_d50b8d18-196a-418b-b34d-e000825e853f" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCommonStockSharesOutstanding_209bb34e-8df9-4cd3-88ca-200e4ae049a1" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityCommonStockSharesOutstanding_209bb34e-8df9-4cd3-88ca-200e4ae049a1" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityWellKnownSeasonedIssuer_d293f852-164e-4de7-bf4c-d55a5dcc8d18" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityWellKnownSeasonedIssuer"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityWellKnownSeasonedIssuer_d293f852-164e-4de7-bf4c-d55a5dcc8d18" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityVoluntaryFilers_fdbbe1eb-07fa-4374-b9b3-e5e5142a35b6" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityVoluntaryFilers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityVoluntaryFilers_fdbbe1eb-07fa-4374-b9b3-e5e5142a35b6" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCurrentReportingStatus_6bac042b-6531-4aff-a5f0-e2e858fc98c6" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCurrentReportingStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityCurrentReportingStatus_6bac042b-6531-4aff-a5f0-e2e858fc98c6" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInteractiveDataCurrent_934ae63c-2250-4979-a8c1-5bff9cb5062e" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityInteractiveDataCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityInteractiveDataCurrent_934ae63c-2250-4979-a8c1-5bff9cb5062e" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFilerCategory_4070f4a6-1acb-405d-8393-9504c88b4951" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityFilerCategory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityFilerCategory_4070f4a6-1acb-405d-8393-9504c88b4951" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_8927eaea-743e-42b4-bfbd-8f714ef9ffe5" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityEmergingGrowthCompany_8927eaea-743e-42b4-bfbd-8f714ef9ffe5" xlink:type="arc" order="28"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_IcfrAuditorAttestationFlag_20ed683d-af72-458a-916d-1c27739385b9" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_IcfrAuditorAttestationFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_IcfrAuditorAttestationFlag_20ed683d-af72-458a-916d-1c27739385b9" xlink:type="arc" order="29"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFinStmtErrorCorrectionFlag_ea835775-be39-46e9-aa01-125ffb59436b" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentFinStmtErrorCorrectionFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_DocumentFinStmtErrorCorrectionFlag_ea835775-be39-46e9-aa01-125ffb59436b" xlink:type="arc" order="30"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentAccountingStandard_9a19a312-bf71-4142-97b1-65f928cf81bc" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentAccountingStandard"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_DocumentAccountingStandard_9a19a312-bf71-4142-97b1-65f928cf81bc" xlink:type="arc" order="31"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityShellCompany_662c3345-a1ba-45ef-b34e-d4210fa1182f" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityShellCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityShellCompany_662c3345-a1ba-45ef-b34e-d4210fa1182f" xlink:type="arc" order="32"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_0f24b889-f4b1-4f1f-a487-3f1f39c37d81" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_AmendmentFlag_0f24b889-f4b1-4f1f-a487-3f1f39c37d81" xlink:type="arc" order="33"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalPeriodFocus_132e36fb-b4de-43c0-885d-a0f983c25668" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentFiscalPeriodFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_DocumentFiscalPeriodFocus_132e36fb-b4de-43c0-885d-a0f983c25668" xlink:type="arc" order="34"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalYearFocus_6688d364-bb47-42b0-9896-b680e1f7920f" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentFiscalYearFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_DocumentFiscalYearFocus_6688d364-bb47-42b0-9896-b680e1f7920f" xlink:type="arc" order="35"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_14def897-b1c0-4a89-9ace-030d79ddd4b9" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_EntityCentralIndexKey_14def897-b1c0-4a89-9ace-030d79ddd4b9" xlink:type="arc" order="36"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AuditorFirmId_9d3648d2-619a-4986-a38d-4cee30868c01" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_AuditorFirmId"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_AuditorFirmId_9d3648d2-619a-4986-a38d-4cee30868c01" xlink:type="arc" order="37"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_NoTradingSymbolFlag_d4d74f78-3271-4496-beb5-e0b140ba9d08" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_NoTradingSymbolFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityListingsLineItems_533a1a19-be69-4d54-adc7-f396ac2d9538" xlink:to="loc_dei_NoTradingSymbolFlag_d4d74f78-3271-4496-beb5-e0b140ba9d08" xlink:type="arc" order="38"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/ConsolidatedStatementsofProfitorLoss" xlink:type="simple" xlink:href="bntx-20241231.xsd#ConsolidatedStatementsofProfitorLoss"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/ConsolidatedStatementsofProfitorLoss" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_EarningsPerShareLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_EarningsPerShareTable_8841aaf0-f01d-4df1-833c-31d90f081c9a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_EarningsPerShareTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_EarningsPerShareTable_8841aaf0-f01d-4df1-833c-31d90f081c9a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_24fb9d8c-5667-464f-9a6e-b637b7f59ed0" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_EarningsPerShareTable_8841aaf0-f01d-4df1-833c-31d90f081c9a" xlink:to="loc_srt_ProductOrServiceAxis_24fb9d8c-5667-464f-9a6e-b637b7f59ed0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_24fb9d8c-5667-464f-9a6e-b637b7f59ed0_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_24fb9d8c-5667-464f-9a6e-b637b7f59ed0" xlink:to="loc_srt_ProductsAndServicesDomain_24fb9d8c-5667-464f-9a6e-b637b7f59ed0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_19a330ff-b2da-4142-b419-c527ccab537e" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_24fb9d8c-5667-464f-9a6e-b637b7f59ed0" xlink:to="loc_srt_ProductsAndServicesDomain_19a330ff-b2da-4142-b419-c527ccab537e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CommercialMember_605edf3e-2406-4ec5-bd61-0cf83f56e880" xlink:href="bntx-20241231.xsd#bntx_CommercialMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_19a330ff-b2da-4142-b419-c527ccab537e" xlink:to="loc_bntx_CommercialMember_605edf3e-2406-4ec5-bd61-0cf83f56e880" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ResearchAndDevelopmentMember_c050adb5-37af-4b6b-9db7-f32c764179b4" xlink:href="bntx-20241231.xsd#bntx_ResearchAndDevelopmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_19a330ff-b2da-4142-b419-c527ccab537e" xlink:to="loc_bntx_ResearchAndDevelopmentMember_c050adb5-37af-4b6b-9db7-f32c764179b4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RevenueFromContractsWithCustomers_1c43a187-9e3a-4f79-8c45-92c844447d71" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RevenueFromContractsWithCustomers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_RevenueFromContractsWithCustomers_1c43a187-9e3a-4f79-8c45-92c844447d71" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CostOfSales_6d3e84ea-6e9a-43a6-9d3e-3bfad2e1d220" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CostOfSales"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_CostOfSales_6d3e84ea-6e9a-43a6-9d3e-3bfad2e1d220" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ResearchAndDevelopmentExpense_e5418f27-72ab-402a-b259-1ed25cbe1066" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ResearchAndDevelopmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_ResearchAndDevelopmentExpense_e5418f27-72ab-402a-b259-1ed25cbe1066" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SalesAndMarketingExpense_e3d532a4-5176-4937-8d5d-8611b6fa5dcd" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SalesAndMarketingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_SalesAndMarketingExpense_e3d532a4-5176-4937-8d5d-8611b6fa5dcd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeneralAndAdministrativeExpense_a3bc770f-21c3-46b8-b19b-49bb6ec293cc" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_GeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_GeneralAndAdministrativeExpense_a3bc770f-21c3-46b8-b19b-49bb6ec293cc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MiscellaneousOtherOperatingExpense_4dbf4bf1-50c2-4fac-96c2-6cee99882d96" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MiscellaneousOtherOperatingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_MiscellaneousOtherOperatingExpense_4dbf4bf1-50c2-4fac-96c2-6cee99882d96" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MiscellaneousOtherOperatingIncome_676abaaa-e88d-4dc8-b9d3-d9fafdebe11f" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MiscellaneousOtherOperatingIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_MiscellaneousOtherOperatingIncome_676abaaa-e88d-4dc8-b9d3-d9fafdebe11f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProfitLossFromOperatingActivities_dcdbff14-7127-4dca-b778-6ee9e0feeeb7" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ProfitLossFromOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_ProfitLossFromOperatingActivities_dcdbff14-7127-4dca-b778-6ee9e0feeeb7" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinanceIncome_5759f03e-7105-484e-acc5-f1bfc8a480cf" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_FinanceIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_FinanceIncome_5759f03e-7105-484e-acc5-f1bfc8a480cf" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinanceCosts_54718937-2d63-4dfa-8ee9-3dc7b93a9f3b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_FinanceCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_FinanceCosts_54718937-2d63-4dfa-8ee9-3dc7b93a9f3b" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProfitLossBeforeTax_85001a90-0093-4a5c-a4f5-f40d3bdaff4b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ProfitLossBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_ProfitLossBeforeTax_85001a90-0093-4a5c-a4f5-f40d3bdaff4b" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncomeTaxExpenseContinuingOperations_5e0050af-20ca-4870-a58f-e446cc30efa8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IncomeTaxExpenseContinuingOperations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_IncomeTaxExpenseContinuingOperations_5e0050af-20ca-4870-a58f-e446cc30efa8" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProfitLoss_cd1e34f6-a3fd-4434-9996-fedb58b2f464" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ProfitLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_ProfitLoss_cd1e34f6-a3fd-4434-9996-fedb58b2f464" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_EarningsPerShareAbstract_340f624d-19d9-49cc-9d74-5a4d7e93456e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_EarningsPerShareAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareLineItems_a9ce2b3d-0bc3-4b5b-8769-3f25a85646a3" xlink:to="loc_ifrs-full_EarningsPerShareAbstract_340f624d-19d9-49cc-9d74-5a4d7e93456e" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BasicEarningsLossPerShare_49f59a76-16e3-49ae-a24e-1adf44fdbf04" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BasicEarningsLossPerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareAbstract_340f624d-19d9-49cc-9d74-5a4d7e93456e" xlink:to="loc_ifrs-full_BasicEarningsLossPerShare_49f59a76-16e3-49ae-a24e-1adf44fdbf04" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DilutedEarningsLossPerShare_0cbad6e5-541f-4eb7-97ce-3518c2cd0a21" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DilutedEarningsLossPerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EarningsPerShareAbstract_340f624d-19d9-49cc-9d74-5a4d7e93456e" xlink:to="loc_ifrs-full_DilutedEarningsLossPerShare_0cbad6e5-541f-4eb7-97ce-3518c2cd0a21" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/ConsolidatedStatementsofChangesinStockholdersEquity" xlink:type="simple" xlink:href="bntx-20241231.xsd#ConsolidatedStatementsofChangesinStockholdersEquity"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/ConsolidatedStatementsofChangesinStockholdersEquity" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_StatementOfChangesInEquityLineItems_90e9fd51-ab00-452f-93be-ecbed6d72fef" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_StatementOfChangesInEquityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_StatementOfChangesInEquityTable_17a07576-1168-4dfe-87cc-b1a1f62fe589" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_StatementOfChangesInEquityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_90e9fd51-ab00-452f-93be-ecbed6d72fef" xlink:to="loc_ifrs-full_StatementOfChangesInEquityTable_17a07576-1168-4dfe-87cc-b1a1f62fe589" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComponentsOfEquityAxis_9a9b77cc-aef7-4828-b66b-25258fc00c12" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ComponentsOfEquityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_StatementOfChangesInEquityTable_17a07576-1168-4dfe-87cc-b1a1f62fe589" xlink:to="loc_ifrs-full_ComponentsOfEquityAxis_9a9b77cc-aef7-4828-b66b-25258fc00c12" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComponentsOfEquityDomain_9a9b77cc-aef7-4828-b66b-25258fc00c12_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ComponentsOfEquityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ComponentsOfEquityAxis_9a9b77cc-aef7-4828-b66b-25258fc00c12" xlink:to="loc_ifrs-full_ComponentsOfEquityDomain_9a9b77cc-aef7-4828-b66b-25258fc00c12_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComponentsOfEquityDomain_fdb21cb0-3bf2-45c9-a51a-3bdcbe22f76b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ComponentsOfEquityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ComponentsOfEquityAxis_9a9b77cc-aef7-4828-b66b-25258fc00c12" xlink:to="loc_ifrs-full_ComponentsOfEquityDomain_fdb21cb0-3bf2-45c9-a51a-3bdcbe22f76b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IssuedCapitalMember_768718d2-5ec7-40da-9b67-68fefaa79213" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IssuedCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfEquityDomain_fdb21cb0-3bf2-45c9-a51a-3bdcbe22f76b" xlink:to="loc_ifrs-full_IssuedCapitalMember_768718d2-5ec7-40da-9b67-68fefaa79213" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CapitalReserveMember_25507481-58f7-4c5d-ae41-18c6ad242dfc" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CapitalReserveMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfEquityDomain_fdb21cb0-3bf2-45c9-a51a-3bdcbe22f76b" xlink:to="loc_ifrs-full_CapitalReserveMember_25507481-58f7-4c5d-ae41-18c6ad242dfc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TreasurySharesMember_e9713ee0-2305-47ce-94ac-cd6171ac522d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TreasurySharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfEquityDomain_fdb21cb0-3bf2-45c9-a51a-3bdcbe22f76b" xlink:to="loc_ifrs-full_TreasurySharesMember_e9713ee0-2305-47ce-94ac-cd6171ac522d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RetainedEarningsMember_2f699284-ed48-48a1-82c9-48a598df826f" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfEquityDomain_fdb21cb0-3bf2-45c9-a51a-3bdcbe22f76b" xlink:to="loc_ifrs-full_RetainedEarningsMember_2f699284-ed48-48a1-82c9-48a598df826f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherReservesMember_0a655064-ba0f-42a6-b326-82064d44df59" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_OtherReservesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfEquityDomain_fdb21cb0-3bf2-45c9-a51a-3bdcbe22f76b" xlink:to="loc_ifrs-full_OtherReservesMember_0a655064-ba0f-42a6-b326-82064d44df59" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Equity_0454e1df-65aa-4cce-8f9f-cefa5b0acf28" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_Equity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_90e9fd51-ab00-452f-93be-ecbed6d72fef" xlink:to="loc_ifrs-full_Equity_0454e1df-65aa-4cce-8f9f-cefa5b0acf28" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProfitLoss_eea5cf2b-105c-4a7e-a665-1d4a5d75f533" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ProfitLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_90e9fd51-ab00-452f-93be-ecbed6d72fef" xlink:to="loc_ifrs-full_ProfitLoss_eea5cf2b-105c-4a7e-a665-1d4a5d75f533" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherComprehensiveIncome_8aa9065b-04fe-497b-a0b5-d1b7d8251fa3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_OtherComprehensiveIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_90e9fd51-ab00-452f-93be-ecbed6d72fef" xlink:to="loc_ifrs-full_OtherComprehensiveIncome_8aa9065b-04fe-497b-a0b5-d1b7d8251fa3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComprehensiveIncome_6c1b1603-9aef-44a0-b4aa-b50f228be857" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ComprehensiveIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_90e9fd51-ab00-452f-93be-ecbed6d72fef" xlink:to="loc_ifrs-full_ComprehensiveIncome_6c1b1603-9aef-44a0-b4aa-b50f228be857" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_IssuanceOfShareCapital_c17eb596-bac5-4777-8620-bd04620d70f6" xlink:href="bntx-20241231.xsd#bntx_IssuanceOfShareCapital"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_90e9fd51-ab00-452f-93be-ecbed6d72fef" xlink:to="loc_bntx_IssuanceOfShareCapital_c17eb596-bac5-4777-8620-bd04620d70f6" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_RedemptionOfConvertibleNote_2ee21969-4eee-41ec-846e-0b35591af463" xlink:href="bntx-20241231.xsd#bntx_RedemptionOfConvertibleNote"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_90e9fd51-ab00-452f-93be-ecbed6d72fef" xlink:to="loc_bntx_RedemptionOfConvertibleNote_2ee21969-4eee-41ec-846e-0b35591af463" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_TreasurySharesUsedForAcquisitionOfBusinessCombination_bfc78e81-614e-4da5-bcea-52d8a4f73456" xlink:href="bntx-20241231.xsd#bntx_TreasurySharesUsedForAcquisitionOfBusinessCombination"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_90e9fd51-ab00-452f-93be-ecbed6d72fef" xlink:to="loc_bntx_TreasurySharesUsedForAcquisitionOfBusinessCombination_bfc78e81-614e-4da5-bcea-52d8a4f73456" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramRepurchasedDuringPeriodValue_c8f48089-7ab4-4297-9b63-7f8a6009a6da" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramRepurchasedDuringPeriodValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_90e9fd51-ab00-452f-93be-ecbed6d72fef" xlink:to="loc_bntx_ShareRepurchaseProgramRepurchasedDuringPeriodValue_c8f48089-7ab4-4297-9b63-7f8a6009a6da" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ShareIssueRelatedCost_38525d24-d484-4362-9f5b-89aaf0645e0a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ShareIssueRelatedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_90e9fd51-ab00-452f-93be-ecbed6d72fef" xlink:to="loc_ifrs-full_ShareIssueRelatedCost_38525d24-d484-4362-9f5b-89aaf0645e0a" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DividendsPaid_7574439e-e40f-492b-868c-b2b339c4f555" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DividendsPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_90e9fd51-ab00-452f-93be-ecbed6d72fef" xlink:to="loc_ifrs-full_DividendsPaid_7574439e-e40f-492b-868c-b2b339c4f555" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughSharebasedPaymentTransactions_403feb80-d33c-4765-a7d1-5cf9ff1a75a4" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IncreaseDecreaseThroughSharebasedPaymentTransactions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_90e9fd51-ab00-452f-93be-ecbed6d72fef" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughSharebasedPaymentTransactions_403feb80-d33c-4765-a7d1-5cf9ff1a75a4" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CurrentAndDeferredTaxExpenseIncome_dc91e385-e125-465e-a28c-a66ee255152e" xlink:href="bntx-20241231.xsd#bntx_CurrentAndDeferredTaxExpenseIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_90e9fd51-ab00-452f-93be-ecbed6d72fef" xlink:to="loc_bntx_CurrentAndDeferredTaxExpenseIncome_dc91e385-e125-465e-a28c-a66ee255152e" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Equity_d27442b8-7ce8-47cd-bba5-e814428f5fe8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_Equity"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/SignificantAccountingPoliciesAdditionalInformationDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#SignificantAccountingPoliciesAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/SignificantAccountingPoliciesAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_f44208fb-9558-4803-9b7a-62a6a9dd4091" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_07596b42-10d3-474e-86ea-23249641f33d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_f44208fb-9558-4803-9b7a-62a6a9dd4091" xlink:to="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_07596b42-10d3-474e-86ea-23249641f33d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_6dc0ac63-f188-4661-a6a2-781a75768ea1" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_07596b42-10d3-474e-86ea-23249641f33d" xlink:to="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_6dc0ac63-f188-4661-a6a2-781a75768ea1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_6dc0ac63-f188-4661-a6a2-781a75768ea1_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_6dc0ac63-f188-4661-a6a2-781a75768ea1" xlink:to="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_6dc0ac63-f188-4661-a6a2-781a75768ea1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_848ddd8f-30f3-46dc-b7b9-82ab14d5d841" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_6dc0ac63-f188-4661-a6a2-781a75768ea1" xlink:to="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_848ddd8f-30f3-46dc-b7b9-82ab14d5d841" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OperatingAndBusinessEquipmentMember_b94533bc-cdf1-4f42-9c9f-78919ff0eaa3" xlink:href="bntx-20241231.xsd#bntx_OperatingAndBusinessEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_848ddd8f-30f3-46dc-b7b9-82ab14d5d841" xlink:to="loc_bntx_OperatingAndBusinessEquipmentMember_b94533bc-cdf1-4f42-9c9f-78919ff0eaa3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeAxis_35ea5072-8b88-44cf-aa36-e1ec4c15b681" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_07596b42-10d3-474e-86ea-23249641f33d" xlink:to="loc_ifrs-full_RangeAxis_35ea5072-8b88-44cf-aa36-e1ec4c15b681" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeDomain_35ea5072-8b88-44cf-aa36-e1ec4c15b681_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_RangeAxis_35ea5072-8b88-44cf-aa36-e1ec4c15b681" xlink:to="loc_ifrs-full_RangeDomain_35ea5072-8b88-44cf-aa36-e1ec4c15b681_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeDomain_62feeda3-c047-44a7-9861-de0264a06c13" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_RangeAxis_35ea5072-8b88-44cf-aa36-e1ec4c15b681" xlink:to="loc_ifrs-full_RangeDomain_62feeda3-c047-44a7-9861-de0264a06c13" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BottomOfRangeMember_2a75a3eb-1a26-490f-a9db-eb9d1b60cad2" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BottomOfRangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RangeDomain_62feeda3-c047-44a7-9861-de0264a06c13" xlink:to="loc_ifrs-full_BottomOfRangeMember_2a75a3eb-1a26-490f-a9db-eb9d1b60cad2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TopOfRangeMember_3f1086a8-fdbe-44ce-883a-8baa1b67c99e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TopOfRangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RangeDomain_62feeda3-c047-44a7-9861-de0264a06c13" xlink:to="loc_ifrs-full_TopOfRangeMember_3f1086a8-fdbe-44ce-883a-8baa1b67c99e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DescriptionOfSignificantPaymentTermsInContractsWithCustomers_c552e07c-77e9-4c11-9b82-6b2c574c8ae5" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DescriptionOfSignificantPaymentTermsInContractsWithCustomers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_f44208fb-9558-4803-9b7a-62a6a9dd4091" xlink:to="loc_ifrs-full_DescriptionOfSignificantPaymentTermsInContractsWithCustomers_c552e07c-77e9-4c11-9b82-6b2c574c8ae5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TaxRateEffectFromChangeInTaxRate_f8e5187b-ca77-4be0-bd78-978c2df6e290" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TaxRateEffectFromChangeInTaxRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_f44208fb-9558-4803-9b7a-62a6a9dd4091" xlink:to="loc_ifrs-full_TaxRateEffectFromChangeInTaxRate_f8e5187b-ca77-4be0-bd78-978c2df6e290" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_UsefulLifeMeasuredAsPeriodOfTimePropertyPlantAndEquipment_f7413d27-677f-472e-b4f0-c589fdec7a8b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_UsefulLifeMeasuredAsPeriodOfTimePropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_f44208fb-9558-4803-9b7a-62a6a9dd4091" xlink:to="loc_ifrs-full_UsefulLifeMeasuredAsPeriodOfTimePropertyPlantAndEquipment_f7413d27-677f-472e-b4f0-c589fdec7a8b" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/SignificantAccountingPoliciesSummaryofUsefulLivesAppliedtotheGroupsIntangibleAssetsDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#SignificantAccountingPoliciesSummaryofUsefulLivesAppliedtotheGroupsIntangibleAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/SignificantAccountingPoliciesSummaryofUsefulLivesAppliedtotheGroupsIntangibleAssetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_f311dcf2-1160-4e55-84ac-d758ba360226" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfIntangibleAssetsTable_44dfa53f-5909-46fa-b6bf-3eb5886b37ea" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_f311dcf2-1160-4e55-84ac-d758ba360226" xlink:to="loc_ifrs-full_DisclosureOfIntangibleAssetsTable_44dfa53f-5909-46fa-b6bf-3eb5886b37ea" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillAxis_cd6e5a9d-517b-41b7-b1a8-24138ddea891" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsTable_44dfa53f-5909-46fa-b6bf-3eb5886b37ea" xlink:to="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillAxis_cd6e5a9d-517b-41b7-b1a8-24138ddea891" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain_cd6e5a9d-517b-41b7-b1a8-24138ddea891_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillAxis_cd6e5a9d-517b-41b7-b1a8-24138ddea891" xlink:to="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain_cd6e5a9d-517b-41b7-b1a8-24138ddea891_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IntangibleAssetsOtherThanGoodwillMember_db7f238c-c3d3-4abd-8ea0-6fb5ba90e582" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IntangibleAssetsOtherThanGoodwillMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillAxis_cd6e5a9d-517b-41b7-b1a8-24138ddea891" xlink:to="loc_ifrs-full_IntangibleAssetsOtherThanGoodwillMember_db7f238c-c3d3-4abd-8ea0-6fb5ba90e582" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_IntellectualPropertyRightsMember_15858518-5014-4228-851f-0858003d10e5" xlink:href="bntx-20241231.xsd#bntx_IntellectualPropertyRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_IntangibleAssetsOtherThanGoodwillMember_db7f238c-c3d3-4abd-8ea0-6fb5ba90e582" xlink:to="loc_bntx_IntellectualPropertyRightsMember_15858518-5014-4228-851f-0858003d10e5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LicencesMember_052a9fa7-3083-4812-8987-c22e6cfa06b1" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LicencesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_IntangibleAssetsOtherThanGoodwillMember_db7f238c-c3d3-4abd-8ea0-6fb5ba90e582" xlink:to="loc_ifrs-full_LicencesMember_052a9fa7-3083-4812-8987-c22e6cfa06b1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComputerSoftwareMember_67d2a962-dcc8-45e3-bc40-d2cc11ad4f00" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ComputerSoftwareMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_IntangibleAssetsOtherThanGoodwillMember_db7f238c-c3d3-4abd-8ea0-6fb5ba90e582" xlink:to="loc_ifrs-full_ComputerSoftwareMember_67d2a962-dcc8-45e3-bc40-d2cc11ad4f00" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeAxis_4bb84c4f-26b3-4c6c-97c9-80a983263832" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsTable_44dfa53f-5909-46fa-b6bf-3eb5886b37ea" xlink:to="loc_ifrs-full_RangeAxis_4bb84c4f-26b3-4c6c-97c9-80a983263832" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeDomain_4bb84c4f-26b3-4c6c-97c9-80a983263832_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_RangeAxis_4bb84c4f-26b3-4c6c-97c9-80a983263832" xlink:to="loc_ifrs-full_RangeDomain_4bb84c4f-26b3-4c6c-97c9-80a983263832_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeDomain_5b377d51-011b-4414-b9e5-1ccc240c995e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_RangeAxis_4bb84c4f-26b3-4c6c-97c9-80a983263832" xlink:to="loc_ifrs-full_RangeDomain_5b377d51-011b-4414-b9e5-1ccc240c995e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BottomOfRangeMember_f679d45b-772c-4e10-8332-2a7fc4546fde" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BottomOfRangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RangeDomain_5b377d51-011b-4414-b9e5-1ccc240c995e" xlink:to="loc_ifrs-full_BottomOfRangeMember_f679d45b-772c-4e10-8332-2a7fc4546fde" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TopOfRangeMember_c5163eb3-6049-43fa-b3b9-25ff474dbb18" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TopOfRangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RangeDomain_5b377d51-011b-4414-b9e5-1ccc240c995e" xlink:to="loc_ifrs-full_TopOfRangeMember_c5163eb3-6049-43fa-b3b9-25ff474dbb18" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_UsefulLifeMeasuredAsPeriodOfTimeIntangibleAssetsOtherThanGoodwill_403cc6aa-7b60-4314-a10b-236b50705032" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_UsefulLifeMeasuredAsPeriodOfTimeIntangibleAssetsOtherThanGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_f311dcf2-1160-4e55-84ac-d758ba360226" xlink:to="loc_ifrs-full_UsefulLifeMeasuredAsPeriodOfTimeIntangibleAssetsOtherThanGoodwill_403cc6aa-7b60-4314-a10b-236b50705032" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/SignificantAccountingPoliciesSummaryofPropertyPlantandEquipmentDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#SignificantAccountingPoliciesSummaryofPropertyPlantandEquipmentDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/SignificantAccountingPoliciesSummaryofPropertyPlantandEquipmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_f49dd931-e18e-40af-b04f-71d0fbb18254" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_a7313345-6ba2-424f-a8eb-207e6dd8df94" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_f49dd931-e18e-40af-b04f-71d0fbb18254" xlink:to="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_a7313345-6ba2-424f-a8eb-207e6dd8df94" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_5f7c038d-e282-4e4c-8f32-8adc1530f425" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_a7313345-6ba2-424f-a8eb-207e6dd8df94" xlink:to="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_5f7c038d-e282-4e4c-8f32-8adc1530f425" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_5f7c038d-e282-4e4c-8f32-8adc1530f425_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_5f7c038d-e282-4e4c-8f32-8adc1530f425" xlink:to="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_5f7c038d-e282-4e4c-8f32-8adc1530f425_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PropertyPlantAndEquipmentMember_26ca0ddc-ae22-4969-8a45-8772e2548bc8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_PropertyPlantAndEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_5f7c038d-e282-4e4c-8f32-8adc1530f425" xlink:to="loc_ifrs-full_PropertyPlantAndEquipmentMember_26ca0ddc-ae22-4969-8a45-8772e2548bc8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BuildingsMember_4a5b4371-e571-49da-ad44-9d11dea3135b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BuildingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_PropertyPlantAndEquipmentMember_26ca0ddc-ae22-4969-8a45-8772e2548bc8" xlink:to="loc_ifrs-full_BuildingsMember_4a5b4371-e571-49da-ad44-9d11dea3135b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EquipmentToolsAndInstallationsMember_ae28512c-797c-4f0b-b7e2-e00e0239fbb2" xlink:href="bntx-20241231.xsd#bntx_EquipmentToolsAndInstallationsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_PropertyPlantAndEquipmentMember_26ca0ddc-ae22-4969-8a45-8772e2548bc8" xlink:to="loc_bntx_EquipmentToolsAndInstallationsMember_ae28512c-797c-4f0b-b7e2-e00e0239fbb2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeAxis_d119f7d2-c0c9-4157-84e3-be93576e0108" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_a7313345-6ba2-424f-a8eb-207e6dd8df94" xlink:to="loc_ifrs-full_RangeAxis_d119f7d2-c0c9-4157-84e3-be93576e0108" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeDomain_d119f7d2-c0c9-4157-84e3-be93576e0108_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_RangeAxis_d119f7d2-c0c9-4157-84e3-be93576e0108" xlink:to="loc_ifrs-full_RangeDomain_d119f7d2-c0c9-4157-84e3-be93576e0108_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeDomain_be23f443-0be5-47ad-88d4-2072563e2c9b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_RangeAxis_d119f7d2-c0c9-4157-84e3-be93576e0108" xlink:to="loc_ifrs-full_RangeDomain_be23f443-0be5-47ad-88d4-2072563e2c9b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BottomOfRangeMember_849a8868-6a30-456e-9e5b-c6aa8e2f569c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BottomOfRangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RangeDomain_be23f443-0be5-47ad-88d4-2072563e2c9b" xlink:to="loc_ifrs-full_BottomOfRangeMember_849a8868-6a30-456e-9e5b-c6aa8e2f569c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TopOfRangeMember_57515fed-7db5-46ee-820d-9c992ab71901" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TopOfRangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RangeDomain_be23f443-0be5-47ad-88d4-2072563e2c9b" xlink:to="loc_ifrs-full_TopOfRangeMember_57515fed-7db5-46ee-820d-9c992ab71901" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_UsefulLifeMeasuredAsPeriodOfTimePropertyPlantAndEquipment_7de0ac08-bc25-4505-85bc-a2a7b207ab84" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_UsefulLifeMeasuredAsPeriodOfTimePropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_f49dd931-e18e-40af-b04f-71d0fbb18254" xlink:to="loc_ifrs-full_UsefulLifeMeasuredAsPeriodOfTimePropertyPlantAndEquipment_7de0ac08-bc25-4505-85bc-a2a7b207ab84" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/SignificantAccountingPoliciesSummaryofEstimatedUsefulLivesoftheAssetsorShorterLeaseTermDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#SignificantAccountingPoliciesSummaryofEstimatedUsefulLivesoftheAssetsorShorterLeaseTermDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/SignificantAccountingPoliciesSummaryofEstimatedUsefulLivesoftheAssetsorShorterLeaseTermDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems_8061c279-cfd3-4f91-ab83-67b30d0d063f" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsTable_0b8c6bd5-5e34-4a22-bf10-34e7c31e9c27" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems_8061c279-cfd3-4f91-ab83-67b30d0d063f" xlink:to="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsTable_0b8c6bd5-5e34-4a22-bf10-34e7c31e9c27" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsAxis_62c456f7-7219-4ad1-871c-c2a5b4f470a8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsTable_0b8c6bd5-5e34-4a22-bf10-34e7c31e9c27" xlink:to="loc_ifrs-full_ClassesOfAssetsAxis_62c456f7-7219-4ad1-871c-c2a5b4f470a8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_62c456f7-7219-4ad1-871c-c2a5b4f470a8_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_62c456f7-7219-4ad1-871c-c2a5b4f470a8" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_62c456f7-7219-4ad1-871c-c2a5b4f470a8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_9762f0c8-84f3-4bdc-a9e6-7bd12ebdd441" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_62c456f7-7219-4ad1-871c-c2a5b4f470a8" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_9762f0c8-84f3-4bdc-a9e6-7bd12ebdd441" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BuildingsMember_1f92fd93-7dd9-4520-bb3e-668d4f123cd3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BuildingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_9762f0c8-84f3-4bdc-a9e6-7bd12ebdd441" xlink:to="loc_ifrs-full_BuildingsMember_1f92fd93-7dd9-4520-bb3e-668d4f123cd3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EquipmentToolsAndInstallationsMember_fb13740a-10db-4c4a-87e1-6dae76591f7b" xlink:href="bntx-20241231.xsd#bntx_EquipmentToolsAndInstallationsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_9762f0c8-84f3-4bdc-a9e6-7bd12ebdd441" xlink:to="loc_bntx_EquipmentToolsAndInstallationsMember_fb13740a-10db-4c4a-87e1-6dae76591f7b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ProductionFacilitiesMember_e855d17c-3e8c-401a-af5f-a27573811d1d" xlink:href="bntx-20241231.xsd#bntx_ProductionFacilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_9762f0c8-84f3-4bdc-a9e6-7bd12ebdd441" xlink:to="loc_bntx_ProductionFacilitiesMember_e855d17c-3e8c-401a-af5f-a27573811d1d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_VehiclesMember_d2fc3970-cf65-4dca-8a22-58505f203c9a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_VehiclesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_9762f0c8-84f3-4bdc-a9e6-7bd12ebdd441" xlink:to="loc_ifrs-full_VehiclesMember_d2fc3970-cf65-4dca-8a22-58505f203c9a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeAxis_97666dd6-0689-412f-8a23-c34d9727dfa2" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsTable_0b8c6bd5-5e34-4a22-bf10-34e7c31e9c27" xlink:to="loc_ifrs-full_RangeAxis_97666dd6-0689-412f-8a23-c34d9727dfa2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeDomain_97666dd6-0689-412f-8a23-c34d9727dfa2_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_RangeAxis_97666dd6-0689-412f-8a23-c34d9727dfa2" xlink:to="loc_ifrs-full_RangeDomain_97666dd6-0689-412f-8a23-c34d9727dfa2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeDomain_ceef7f8d-9180-4104-ae4a-a4220063515e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_RangeAxis_97666dd6-0689-412f-8a23-c34d9727dfa2" xlink:to="loc_ifrs-full_RangeDomain_ceef7f8d-9180-4104-ae4a-a4220063515e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BottomOfRangeMember_86ff0656-17a0-44e9-9be5-80fcf7abfad3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BottomOfRangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RangeDomain_ceef7f8d-9180-4104-ae4a-a4220063515e" xlink:to="loc_ifrs-full_BottomOfRangeMember_86ff0656-17a0-44e9-9be5-80fcf7abfad3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TopOfRangeMember_f050cda0-7031-464d-b98e-a8615e5df31f" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TopOfRangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RangeDomain_ceef7f8d-9180-4104-ae4a-a4220063515e" xlink:to="loc_ifrs-full_TopOfRangeMember_f050cda0-7031-464d-b98e-a8615e5df31f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_UsefulLifeMeasuredAsPeriodOfTimeRightOfUseAssets_96210fc7-8aac-42d5-a9e6-7fb443eec582" xlink:href="bntx-20241231.xsd#bntx_UsefulLifeMeasuredAsPeriodOfTimeRightOfUseAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems_8061c279-cfd3-4f91-ab83-67b30d0d063f" xlink:to="loc_bntx_UsefulLifeMeasuredAsPeriodOfTimeRightOfUseAssets_96210fc7-8aac-42d5-a9e6-7fb443eec582" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/GroupInformationSummaryofInformationaboutSubsidiariesDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#GroupInformationSummaryofInformationaboutSubsidiariesDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/GroupInformationSummaryofInformationaboutSubsidiariesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_845f8c8b-4887-4d0d-8d95-86643d929e2d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_ff7090d4-b97b-4cd5-9be4-fb53d514d04e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_845f8c8b-4887-4d0d-8d95-86643d929e2d" xlink:to="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_ff7090d4-b97b-4cd5-9be4-fb53d514d04e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInSubsidiariesAxis_5abea0bc-08aa-43a3-8a93-3a9b59a8b51b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SignificantInvestmentsInSubsidiariesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_ff7090d4-b97b-4cd5-9be4-fb53d514d04e" xlink:to="loc_ifrs-full_SignificantInvestmentsInSubsidiariesAxis_5abea0bc-08aa-43a3-8a93-3a9b59a8b51b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_5abea0bc-08aa-43a3-8a93-3a9b59a8b51b_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SignificantInvestmentsInSubsidiariesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesAxis_5abea0bc-08aa-43a3-8a93-3a9b59a8b51b" xlink:to="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_5abea0bc-08aa-43a3-8a93-3a9b59a8b51b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SignificantInvestmentsInSubsidiariesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesAxis_5abea0bc-08aa-43a3-8a93-3a9b59a8b51b" xlink:to="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechBioNTainerHoldingGmbHMember_1db4050a-b455-477b-9856-c1547e125faf" xlink:href="bntx-20241231.xsd#bntx_BioNTechBioNTainerHoldingGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechBioNTainerHoldingGmbHMember_1db4050a-b455-477b-9856-c1547e125faf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechCellGeneTherapiesGmbHMember_5bef4fe3-6450-46b2-9f13-4152fde8b5ed" xlink:href="bntx-20241231.xsd#bntx_BioNTechCellGeneTherapiesGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechCellGeneTherapiesGmbHMember_5bef4fe3-6450-46b2-9f13-4152fde8b5ed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechDeliveryTechnologiesGmbHMember_b079d36e-bd9d-4fc9-ad53-6808586e9adf" xlink:href="bntx-20241231.xsd#bntx_BioNTechDeliveryTechnologiesGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechDeliveryTechnologiesGmbHMember_b079d36e-bd9d-4fc9-ad53-6808586e9adf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechDiagnosticsGmbHMember_b4359234-228b-4880-8f8c-035b22a46a0b" xlink:href="bntx-20241231.xsd#bntx_BioNTechDiagnosticsGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechDiagnosticsGmbHMember_b4359234-228b-4880-8f8c-035b22a46a0b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechEuropeGmbHMember_8121d464-b07a-481f-8a89-52b86c6353c8" xlink:href="bntx-20241231.xsd#bntx_BioNTechEuropeGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechEuropeGmbHMember_8121d464-b07a-481f-8a89-52b86c6353c8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechIdarObersteinServicesGmbHMember_2c67d16c-9257-4ec1-b320-7c4e74e8baf5" xlink:href="bntx-20241231.xsd#bntx_BioNTechIdarObersteinServicesGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechIdarObersteinServicesGmbHMember_2c67d16c-9257-4ec1-b320-7c4e74e8baf5" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechIndividualizedMRNAManufacturingGmbHMember_4bd496d0-75ec-4ba9-a2e6-df182908fea5" xlink:href="bntx-20241231.xsd#bntx_BioNTechIndividualizedMRNAManufacturingGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechIndividualizedMRNAManufacturingGmbHMember_4bd496d0-75ec-4ba9-a2e6-df182908fea5" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechInnovationAndServicesMarburgGmbHMember_bb12f5c6-3f22-43c8-a4aa-c5c1e5c00c43" xlink:href="bntx-20241231.xsd#bntx_BioNTechInnovationAndServicesMarburgGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechInnovationAndServicesMarburgGmbHMember_bb12f5c6-3f22-43c8-a4aa-c5c1e5c00c43" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechInnovationGmbHMember_73d440e9-804e-4a7e-83e2-9a44f8485e7a" xlink:href="bntx-20241231.xsd#bntx_BioNTechInnovationGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechInnovationGmbHMember_73d440e9-804e-4a7e-83e2-9a44f8485e7a" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechInnovativeManufacturingServicesGmbHMember_c3fb1612-3713-4c85-b2bb-89237b4f387a" xlink:href="bntx-20241231.xsd#bntx_BioNTechInnovativeManufacturingServicesGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechInnovativeManufacturingServicesGmbHMember_c3fb1612-3713-4c85-b2bb-89237b4f387a" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechManufacturingGmbHMember_6ab797d6-c77f-4ac9-9093-2254524951dc" xlink:href="bntx-20241231.xsd#bntx_BioNTechManufacturingGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechManufacturingGmbHMember_6ab797d6-c77f-4ac9-9093-2254524951dc" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechManufacturingMarburgGmbHMember_8c7b7217-ccbf-4b4d-9c7e-749c278fa844" xlink:href="bntx-20241231.xsd#bntx_BioNTechManufacturingMarburgGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechManufacturingMarburgGmbHMember_8c7b7217-ccbf-4b4d-9c7e-749c278fa844" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechRealEstateHoldingGmbHMember_451661d0-c147-48c4-909b-1cf2685114ed" xlink:href="bntx-20241231.xsd#bntx_BioNTechRealEstateHoldingGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechRealEstateHoldingGmbHMember_451661d0-c147-48c4-909b-1cf2685114ed" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechRealEstateVerwaltungsGmbHMember_0db79967-60ac-451a-914a-a9744ac3c1e3" xlink:href="bntx-20241231.xsd#bntx_BioNTechRealEstateVerwaltungsGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechRealEstateVerwaltungsGmbHMember_0db79967-60ac-451a-914a-a9744ac3c1e3" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_InstaDeepDEGmbHMember_e60997c6-a013-41d8-84d8-3fea00ecc905" xlink:href="bntx-20241231.xsd#bntx_InstaDeepDEGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_InstaDeepDEGmbHMember_e60997c6-a013-41d8-84d8-3fea00ecc905" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_JPTPeptideTechnologiesGmbHMember_92b1568f-b7ad-4d8a-a18f-a2b2d3aab815" xlink:href="bntx-20241231.xsd#bntx_JPTPeptideTechnologiesGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_JPTPeptideTechnologiesGmbHMember_92b1568f-b7ad-4d8a-a18f-a2b2d3aab815" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NTSecurityAndServicesGmbHMember_b636cfd8-f204-46fe-9866-a21565975b80" xlink:href="bntx-20241231.xsd#bntx_NTSecurityAndServicesGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_NTSecurityAndServicesGmbHMember_b636cfd8-f204-46fe-9866-a21565975b80" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ReSanoGmbHMember_6a25a511-6b4e-47c0-8317-cc958e24df87" xlink:href="bntx-20241231.xsd#bntx_ReSanoGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_ReSanoGmbHMember_6a25a511-6b4e-47c0-8317-cc958e24df87" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechAustraliaPtyLtd.Member_12055cc8-6a63-42d8-a784-3ee21f71df96" xlink:href="bntx-20241231.xsd#bntx_BioNTechAustraliaPtyLtd.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechAustraliaPtyLtd.Member_12055cc8-6a63-42d8-a784-3ee21f71df96" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechRDAustriaGmbHMember_2659bcb2-8938-448d-95b7-89d279c5340a" xlink:href="bntx-20241231.xsd#bntx_BioNTechRDAustriaGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechRDAustriaGmbHMember_2659bcb2-8938-448d-95b7-89d279c5340a" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechShanghaiPharmaceuticalsCo.Ltd.Member_f4287161-f2c7-46b2-8a1d-ec614d66b2af" xlink:href="bntx-20241231.xsd#bntx_BioNTechShanghaiPharmaceuticalsCo.Ltd.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechShanghaiPharmaceuticalsCo.Ltd.Member_f4287161-f2c7-46b2-8a1d-ec614d66b2af" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_InstaDeepFranceSASMember_63f9231f-5b42-4378-8c1c-c62ef281667a" xlink:href="bntx-20241231.xsd#bntx_InstaDeepFranceSASMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_InstaDeepFranceSASMember_63f9231f-5b42-4378-8c1c-c62ef281667a" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BiopharmaBioNTechIsraelLtd.Member_983eff2c-872e-4b3d-9a99-234bd6116b10" xlink:href="bntx-20241231.xsd#bntx_BiopharmaBioNTechIsraelLtd.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BiopharmaBioNTechIsraelLtd.Member_983eff2c-872e-4b3d-9a99-234bd6116b10" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NewTechnologiesReMember_d3eaf5bd-b32d-48ed-bf44-6ff7320c137c" xlink:href="bntx-20241231.xsd#bntx_NewTechnologiesReMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_NewTechnologiesReMember_d3eaf5bd-b32d-48ed-bf44-6ff7320c137c" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_InstaDeepNigeriaLimitedMember_17044a8e-f273-406f-aa3c-add62dc551c6" xlink:href="bntx-20241231.xsd#bntx_InstaDeepNigeriaLimitedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_InstaDeepNigeriaLimitedMember_17044a8e-f273-406f-aa3c-add62dc551c6" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechRwandaLtd.Member_bd4ae906-d7c5-474c-94a9-0c0e529b1120" xlink:href="bntx-20241231.xsd#bntx_BioNTechRwandaLtd.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechRwandaLtd.Member_bd4ae906-d7c5-474c-94a9-0c0e529b1120" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechSenegalSuarlMember_53643b3a-06dc-4447-9bf6-7fbf8bc24136" xlink:href="bntx-20241231.xsd#bntx_BioNTechSenegalSuarlMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechSenegalSuarlMember_53643b3a-06dc-4447-9bf6-7fbf8bc24136" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechPharmaceuticalsAsiaPacificPte.Ltd.Member_885fbecd-8ffb-4d42-9c09-d069fa536349" xlink:href="bntx-20241231.xsd#bntx_BioNTechPharmaceuticalsAsiaPacificPte.Ltd.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechPharmaceuticalsAsiaPacificPte.Ltd.Member_885fbecd-8ffb-4d42-9c09-d069fa536349" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechPharmaceuticalsSpainS.LMember_6affd4a5-111c-41ac-ac74-f9bf481e614b" xlink:href="bntx-20241231.xsd#bntx_BioNTechPharmaceuticalsSpainS.LMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechPharmaceuticalsSpainS.LMember_6affd4a5-111c-41ac-ac74-f9bf481e614b" xlink:type="arc" order="28"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechSwitzerlandGmbHMember_58ecc5cf-3b92-451b-981e-cb54c113580e" xlink:href="bntx-20241231.xsd#bntx_BioNTechSwitzerlandGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechSwitzerlandGmbHMember_58ecc5cf-3b92-451b-981e-cb54c113580e" xlink:type="arc" order="29"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechTaiwanCo.Ltd.Member_3c080900-3ccf-4ae3-a1f1-40f1c37fd570" xlink:href="bntx-20241231.xsd#bntx_BioNTechTaiwanCo.Ltd.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechTaiwanCo.Ltd.Member_3c080900-3ccf-4ae3-a1f1-40f1c37fd570" xlink:type="arc" order="30"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_InstaDeepTunisiaSARLMember_e38b0aa5-0335-41d3-8a02-7fa18efc0e79" xlink:href="bntx-20241231.xsd#bntx_InstaDeepTunisiaSARLMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_InstaDeepTunisiaSARLMember_e38b0aa5-0335-41d3-8a02-7fa18efc0e79" xlink:type="arc" order="31"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechTurkeyTbbiUrunlerVeKlinikArastirmaTicaretAnonimSirketiMember_ee6dc0f9-5776-4c42-87da-9ce04476d3a3" xlink:href="bntx-20241231.xsd#bntx_BioNTechTurkeyTbbiUrunlerVeKlinikArastirmaTicaretAnonimSirketiMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechTurkeyTbbiUrunlerVeKlinikArastirmaTicaretAnonimSirketiMember_ee6dc0f9-5776-4c42-87da-9ce04476d3a3" xlink:type="arc" order="32"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechUKLtd.Member_c3a00609-9604-4895-9430-3ca2329aa5c0" xlink:href="bntx-20241231.xsd#bntx_BioNTechUKLtd.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechUKLtd.Member_c3a00609-9604-4895-9430-3ca2329aa5c0" xlink:type="arc" order="33"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_InstaDeepLtd.Member_67326cb3-d725-4cd2-989b-05d161e68ec3" xlink:href="bntx-20241231.xsd#bntx_InstaDeepLtd.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_InstaDeepLtd.Member_67326cb3-d725-4cd2-989b-05d161e68ec3" xlink:type="arc" order="34"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechResearchAndDevelopmentInc.Member_ee1e5821-a3d4-4ae3-bab7-6761130fcda1" xlink:href="bntx-20241231.xsd#bntx_BioNTechResearchAndDevelopmentInc.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechResearchAndDevelopmentInc.Member_ee1e5821-a3d4-4ae3-bab7-6761130fcda1" xlink:type="arc" order="35"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechUSAHoldingLLCMember_3409bbe9-e089-4798-9cae-d20f0a5936f2" xlink:href="bntx-20241231.xsd#bntx_BioNTechUSAHoldingLLCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechUSAHoldingLLCMember_3409bbe9-e089-4798-9cae-d20f0a5936f2" xlink:type="arc" order="36"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechUSInc.Member_4ad417b8-bd04-4a12-8c62-86f9eb9014a9" xlink:href="bntx-20241231.xsd#bntx_BioNTechUSInc.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechUSInc.Member_4ad417b8-bd04-4a12-8c62-86f9eb9014a9" xlink:type="arc" order="37"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechDeliveryTechnologiesUSLLCMember_6c230dad-c3db-421e-8c93-ea6c449a5a48" xlink:href="bntx-20241231.xsd#bntx_BioNTechDeliveryTechnologiesUSLLCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechDeliveryTechnologiesUSLLCMember_6c230dad-c3db-421e-8c93-ea6c449a5a48" xlink:type="arc" order="38"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_InstaDeepLLCMember_4ed030b4-87a3-4f7f-bd02-1cf355aee5f1" xlink:href="bntx-20241231.xsd#bntx_InstaDeepLLCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_InstaDeepLLCMember_4ed030b4-87a3-4f7f-bd02-1cf355aee5f1" xlink:type="arc" order="39"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_JPTPeptideTechnologiesInc.Member_49e6522f-286d-44c9-aa27-e09901277ce2" xlink:href="bntx-20241231.xsd#bntx_JPTPeptideTechnologiesInc.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_JPTPeptideTechnologiesInc.Member_49e6522f-286d-44c9-aa27-e09901277ce2" xlink:type="arc" order="40"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechCollaborationsGmbHMember_7a69d130-b518-46f5-ba80-aa7fb16a49e5" xlink:href="bntx-20241231.xsd#bntx_BioNTechCollaborationsGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_BioNTechCollaborationsGmbHMember_7a69d130-b518-46f5-ba80-aa7fb16a49e5" xlink:type="arc" order="41"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_SimbaMergerSubMember_3685fd2d-c5aa-4d3f-b1f6-1792b11a431e" xlink:href="bntx-20241231.xsd#bntx_SimbaMergerSubMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_173af286-44c4-48a1-b53b-d13c4a5501a7" xlink:to="loc_bntx_SimbaMergerSubMember_3685fd2d-c5aa-4d3f-b1f6-1792b11a431e" xlink:type="arc" order="42"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProportionOfOwnershipInterestInSubsidiary_d074c872-117a-452f-80f0-c1712dc60158" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ProportionOfOwnershipInterestInSubsidiary"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_845f8c8b-4887-4d0d-8d95-86643d929e2d" xlink:to="loc_ifrs-full_ProportionOfOwnershipInterestInSubsidiary_d074c872-117a-452f-80f0-c1712dc60158" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/GroupInformationSummaryofInformationaboutParentCompanyDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#GroupInformationSummaryofInformationaboutParentCompanyDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/GroupInformationSummaryofInformationaboutParentCompanyDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_9538c1d0-9198-4cf8-b164-9a2350f69e6d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_def880ad-7654-4300-be4c-9d697ae61fc9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_9538c1d0-9198-4cf8-b164-9a2350f69e6d" xlink:to="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_def880ad-7654-4300-be4c-9d697ae61fc9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipAxis_0beafaff-0ba7-4d30-92ee-fe0aa6b813a5" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_def880ad-7654-4300-be4c-9d697ae61fc9" xlink:to="loc_srt_OwnershipAxis_0beafaff-0ba7-4d30-92ee-fe0aa6b813a5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_0beafaff-0ba7-4d30-92ee-fe0aa6b813a5_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_OwnershipAxis_0beafaff-0ba7-4d30-92ee-fe0aa6b813a5" xlink:to="loc_srt_OwnershipDomain_0beafaff-0ba7-4d30-92ee-fe0aa6b813a5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_c1f31b1e-14f6-4946-8488-b3226d47b91c" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_OwnershipAxis_0beafaff-0ba7-4d30-92ee-fe0aa6b813a5" xlink:to="loc_srt_OwnershipDomain_c1f31b1e-14f6-4946-8488-b3226d47b91c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechMember_32e468b8-2c79-48f1-b6c0-211eec6b884f" xlink:href="bntx-20241231.xsd#bntx_BioNTechMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_OwnershipDomain_c1f31b1e-14f6-4946-8488-b3226d47b91c" xlink:to="loc_bntx_BioNTechMember_32e468b8-2c79-48f1-b6c0-211eec6b884f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_efb478f8-2af8-4d7d-a965-5bb1f389474a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_def880ad-7654-4300-be4c-9d697ae61fc9" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_efb478f8-2af8-4d7d-a965-5bb1f389474a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_efb478f8-2af8-4d7d-a965-5bb1f389474a_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_efb478f8-2af8-4d7d-a965-5bb1f389474a" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_efb478f8-2af8-4d7d-a965-5bb1f389474a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_31239fb6-e477-4ab3-9611-f728a5253648" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_efb478f8-2af8-4d7d-a965-5bb1f389474a" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_31239fb6-e477-4ab3-9611-f728a5253648" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ATImpfGmbHMember_4b17b2b3-6e4a-4749-96ea-18f31cb43a71" xlink:href="bntx-20241231.xsd#bntx_ATImpfGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_31239fb6-e477-4ab3-9611-f728a5253648" xlink:to="loc_bntx_ATImpfGmbHMember_4b17b2b3-6e4a-4749-96ea-18f31cb43a71" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProportionOfOwnershipInterestInSubsidiary_74c9d6a5-00a6-4bef-9bf2-5c9f81a575fa" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ProportionOfOwnershipInterestInSubsidiary"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_9538c1d0-9198-4cf8-b164-9a2350f69e6d" xlink:to="loc_ifrs-full_ProportionOfOwnershipInterestInSubsidiary_74c9d6a5-00a6-4bef-9bf2-5c9f81a575fa" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/GroupInformationSummaryofInformationaboutEntitywithSignificantInfluenceOverGroupDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#GroupInformationSummaryofInformationaboutEntitywithSignificantInfluenceOverGroupDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/GroupInformationSummaryofInformationaboutEntitywithSignificantInfluenceOverGroupDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_65dcbcf1-4148-4f21-88a4-78ff2dab2abe" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_5ebb613a-2003-447e-929d-c81f6f02b704" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_65dcbcf1-4148-4f21-88a4-78ff2dab2abe" xlink:to="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_5ebb613a-2003-447e-929d-c81f6f02b704" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipAxis_82ed978e-d40e-4130-8d77-2dfdaa630c51" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_5ebb613a-2003-447e-929d-c81f6f02b704" xlink:to="loc_srt_OwnershipAxis_82ed978e-d40e-4130-8d77-2dfdaa630c51" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_82ed978e-d40e-4130-8d77-2dfdaa630c51_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_OwnershipAxis_82ed978e-d40e-4130-8d77-2dfdaa630c51" xlink:to="loc_srt_OwnershipDomain_82ed978e-d40e-4130-8d77-2dfdaa630c51_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_bcd2ca55-df20-4d91-be59-58343e31e7a2" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_OwnershipAxis_82ed978e-d40e-4130-8d77-2dfdaa630c51" xlink:to="loc_srt_OwnershipDomain_bcd2ca55-df20-4d91-be59-58343e31e7a2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechMember_b7ade903-a6e0-40c4-821e-b41f3fc065bd" xlink:href="bntx-20241231.xsd#bntx_BioNTechMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_OwnershipDomain_bcd2ca55-df20-4d91-be59-58343e31e7a2" xlink:to="loc_bntx_BioNTechMember_b7ade903-a6e0-40c4-821e-b41f3fc065bd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_e48c23ec-2c52-473f-b835-bc1dc6b66ffd" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_5ebb613a-2003-447e-929d-c81f6f02b704" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_e48c23ec-2c52-473f-b835-bc1dc6b66ffd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_e48c23ec-2c52-473f-b835-bc1dc6b66ffd_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_e48c23ec-2c52-473f-b835-bc1dc6b66ffd" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_e48c23ec-2c52-473f-b835-bc1dc6b66ffd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_9c2b1c93-fdfd-4a26-a1b8-8b99b904053e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_e48c23ec-2c52-473f-b835-bc1dc6b66ffd" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_9c2b1c93-fdfd-4a26-a1b8-8b99b904053e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_MedineGmbHMember_d0482059-ee20-498b-97a4-5ccd72723444" xlink:href="bntx-20241231.xsd#bntx_MedineGmbHMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_9c2b1c93-fdfd-4a26-a1b8-8b99b904053e" xlink:to="loc_bntx_MedineGmbHMember_d0482059-ee20-498b-97a4-5ccd72723444" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProportionOfOwnershipInterestInSubsidiary_6a0e1440-1cec-40ff-b82e-39789aaec46e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ProportionOfOwnershipInterestInSubsidiary"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_65dcbcf1-4148-4f21-88a4-78ff2dab2abe" xlink:to="loc_ifrs-full_ProportionOfOwnershipInterestInSubsidiary_6a0e1440-1cec-40ff-b82e-39789aaec46e" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/BusinessCombinationsAdditionalInformationDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#BusinessCombinationsAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/BusinessCombinationsAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfBusinessCombinationsLineItems_1cb78a36-e935-47fe-8ef2-7676abf00f90" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfBusinessCombinationsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfBusinessCombinationsTable_f18d0e11-d96c-47f1-905d-925a9357d36a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfBusinessCombinationsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfBusinessCombinationsLineItems_1cb78a36-e935-47fe-8ef2-7676abf00f90" xlink:to="loc_ifrs-full_DisclosureOfBusinessCombinationsTable_f18d0e11-d96c-47f1-905d-925a9357d36a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsAxis_c406a179-0359-43ed-85a8-e823c81aa5f8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BusinessCombinationsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfBusinessCombinationsTable_f18d0e11-d96c-47f1-905d-925a9357d36a" xlink:to="loc_ifrs-full_BusinessCombinationsAxis_c406a179-0359-43ed-85a8-e823c81aa5f8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsDomain_c406a179-0359-43ed-85a8-e823c81aa5f8_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BusinessCombinationsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_BusinessCombinationsAxis_c406a179-0359-43ed-85a8-e823c81aa5f8" xlink:to="loc_ifrs-full_BusinessCombinationsDomain_c406a179-0359-43ed-85a8-e823c81aa5f8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsDomain_c196615c-c47c-403c-95f2-c3ba67f0e8da" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BusinessCombinationsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_BusinessCombinationsAxis_c406a179-0359-43ed-85a8-e823c81aa5f8" xlink:to="loc_ifrs-full_BusinessCombinationsDomain_c196615c-c47c-403c-95f2-c3ba67f0e8da" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BiotheusIncMember_b6c1ecac-51ba-4132-bc45-d09e76d7c7d0" xlink:href="bntx-20241231.xsd#bntx_BiotheusIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_BusinessCombinationsDomain_c196615c-c47c-403c-95f2-c3ba67f0e8da" xlink:to="loc_bntx_BiotheusIncMember_b6c1ecac-51ba-4132-bc45-d09e76d7c7d0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_82a08c78-2e3e-48c6-be4b-ebe73c085035" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfBusinessCombinationsTable_f18d0e11-d96c-47f1-905d-925a9357d36a" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_82a08c78-2e3e-48c6-be4b-ebe73c085035" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_82a08c78-2e3e-48c6-be4b-ebe73c085035_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_82a08c78-2e3e-48c6-be4b-ebe73c085035" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_82a08c78-2e3e-48c6-be4b-ebe73c085035_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_bbbd5707-e66d-4faf-be06-f36dc5b3c75a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_82a08c78-2e3e-48c6-be4b-ebe73c085035" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_bbbd5707-e66d-4faf-be06-f36dc5b3c75a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MajorBusinessCombinationMember_52e28bcd-34fe-4102-b425-e05f21640cc0" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MajorBusinessCombinationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_bbbd5707-e66d-4faf-be06-f36dc5b3c75a" xlink:to="loc_ifrs-full_MajorBusinessCombinationMember_52e28bcd-34fe-4102-b425-e05f21640cc0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeAxis_34c131a6-a62c-4e74-a323-9b58258cd870" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfBusinessCombinationsTable_f18d0e11-d96c-47f1-905d-925a9357d36a" xlink:to="loc_ifrs-full_RangeAxis_34c131a6-a62c-4e74-a323-9b58258cd870" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeDomain_34c131a6-a62c-4e74-a323-9b58258cd870_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_RangeAxis_34c131a6-a62c-4e74-a323-9b58258cd870" xlink:to="loc_ifrs-full_RangeDomain_34c131a6-a62c-4e74-a323-9b58258cd870_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RangeDomain_b4e62d1e-6605-40a0-acb2-d27bd17ef655" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RangeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_RangeAxis_34c131a6-a62c-4e74-a323-9b58258cd870" xlink:to="loc_ifrs-full_RangeDomain_b4e62d1e-6605-40a0-acb2-d27bd17ef655" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_79a4f73e-0d27-499b-8356-c535ed8e1e38" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RangeDomain_b4e62d1e-6605-40a0-acb2-d27bd17ef655" xlink:to="loc_srt_MaximumMember_79a4f73e-0d27-499b-8356-c535ed8e1e38" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AcquisitiondateFairValueOfTotalConsiderationTransferred_da553915-e8ab-4c2c-a59f-216d62d6ed8d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AcquisitiondateFairValueOfTotalConsiderationTransferred"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfBusinessCombinationsLineItems_1cb78a36-e935-47fe-8ef2-7676abf00f90" xlink:to="loc_ifrs-full_AcquisitiondateFairValueOfTotalConsiderationTransferred_da553915-e8ab-4c2c-a59f-216d62d6ed8d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PercentageOfVotingEquityInterestsAcquired_649b825d-2972-487b-a722-cb374c9e7f55" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_PercentageOfVotingEquityInterestsAcquired"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfBusinessCombinationsLineItems_1cb78a36-e935-47fe-8ef2-7676abf00f90" xlink:to="loc_ifrs-full_PercentageOfVotingEquityInterestsAcquired_649b825d-2972-487b-a722-cb374c9e7f55" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ContingentConsiderationRecognisedAsOfAcquisitionDate_4b721fb1-812a-4283-95f5-dec0ecd1e5f0" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ContingentConsiderationRecognisedAsOfAcquisitionDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfBusinessCombinationsLineItems_1cb78a36-e935-47fe-8ef2-7676abf00f90" xlink:to="loc_ifrs-full_ContingentConsiderationRecognisedAsOfAcquisitionDate_4b721fb1-812a-4283-95f5-dec0ecd1e5f0" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/RevenuesfromContractswithCustomersSummaryofDisaggregationofRevenuesfromContractswithCustomersDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#RevenuesfromContractswithCustomersSummaryofDisaggregationofRevenuesfromContractswithCustomersDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/RevenuesfromContractswithCustomersSummaryofDisaggregationofRevenuesfromContractswithCustomersDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersLineItems_452dfeb1-5e5d-48ef-af09-3d1be5c7538e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable_d9aa2c8f-785b-49ea-b9ae-b64bcf3b57eb" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersLineItems_452dfeb1-5e5d-48ef-af09-3d1be5c7538e" xlink:to="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable_d9aa2c8f-785b-49ea-b9ae-b64bcf3b57eb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_8964455a-5b2e-4f54-a8a4-e3ef4e23c5a1" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable_d9aa2c8f-785b-49ea-b9ae-b64bcf3b57eb" xlink:to="loc_srt_ProductOrServiceAxis_8964455a-5b2e-4f54-a8a4-e3ef4e23c5a1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_8964455a-5b2e-4f54-a8a4-e3ef4e23c5a1_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_8964455a-5b2e-4f54-a8a4-e3ef4e23c5a1" xlink:to="loc_srt_ProductsAndServicesDomain_8964455a-5b2e-4f54-a8a4-e3ef4e23c5a1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_00391dc9-9807-4d44-91f9-9c0647c638b8" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_8964455a-5b2e-4f54-a8a4-e3ef4e23c5a1" xlink:to="loc_srt_ProductsAndServicesDomain_00391dc9-9807-4d44-91f9-9c0647c638b8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_COVID19VaccineRevenuesMember_fd41e1e4-3e9e-4bb9-9552-baa0add9327a" xlink:href="bntx-20241231.xsd#bntx_COVID19VaccineRevenuesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_00391dc9-9807-4d44-91f9-9c0647c638b8" xlink:to="loc_bntx_COVID19VaccineRevenuesMember_fd41e1e4-3e9e-4bb9-9552-baa0add9327a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OtherSalesMember_cd885fa4-6ab6-4396-a232-da342673bb4f" xlink:href="bntx-20241231.xsd#bntx_OtherSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_00391dc9-9807-4d44-91f9-9c0647c638b8" xlink:to="loc_bntx_OtherSalesMember_cd885fa4-6ab6-4396-a232-da342673bb4f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MajorCustomersAxis_67b2279c-ba49-420d-9003-d8370644267c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MajorCustomersAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable_d9aa2c8f-785b-49ea-b9ae-b64bcf3b57eb" xlink:to="loc_ifrs-full_MajorCustomersAxis_67b2279c-ba49-420d-9003-d8370644267c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MajorCustomersDomain_67b2279c-ba49-420d-9003-d8370644267c_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MajorCustomersDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_MajorCustomersAxis_67b2279c-ba49-420d-9003-d8370644267c" xlink:to="loc_ifrs-full_MajorCustomersDomain_67b2279c-ba49-420d-9003-d8370644267c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MajorCustomersDomain_6c6b85ef-b60e-4200-9b29-eba3341f70ac" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MajorCustomersDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_MajorCustomersAxis_67b2279c-ba49-420d-9003-d8370644267c" xlink:to="loc_ifrs-full_MajorCustomersDomain_6c6b85ef-b60e-4200-9b29-eba3341f70ac" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PfizerIncMember_0a3f76cd-8768-4301-b172-3a018753ebe7" xlink:href="bntx-20241231.xsd#bntx_PfizerIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MajorCustomersDomain_6c6b85ef-b60e-4200-9b29-eba3341f70ac" xlink:to="loc_bntx_PfizerIncMember_0a3f76cd-8768-4301-b172-3a018753ebe7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_GermanFederalMinistryOfHealthMember_ee655298-a292-4ea2-a407-cc116c4b9b8c" xlink:href="bntx-20241231.xsd#bntx_GermanFederalMinistryOfHealthMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MajorCustomersDomain_6c6b85ef-b60e-4200-9b29-eba3341f70ac" xlink:to="loc_bntx_GermanFederalMinistryOfHealthMember_ee655298-a292-4ea2-a407-cc116c4b9b8c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OtherCustomersMember_4ec52195-47b9-4be5-9865-2d83e167bbee" xlink:href="bntx-20241231.xsd#bntx_OtherCustomersMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MajorCustomersDomain_6c6b85ef-b60e-4200-9b29-eba3341f70ac" xlink:to="loc_bntx_OtherCustomersMember_4ec52195-47b9-4be5-9865-2d83e167bbee" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_0458ec84-70b8-4b58-9ba1-c3341e9a2445" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable_d9aa2c8f-785b-49ea-b9ae-b64bcf3b57eb" xlink:to="loc_srt_StatementGeographicalAxis_0458ec84-70b8-4b58-9ba1-c3341e9a2445" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_0458ec84-70b8-4b58-9ba1-c3341e9a2445_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_0458ec84-70b8-4b58-9ba1-c3341e9a2445" xlink:to="loc_srt_SegmentGeographicalDomain_0458ec84-70b8-4b58-9ba1-c3341e9a2445_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_aef764dd-df2f-48f8-bbf7-1062a7691499" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_0458ec84-70b8-4b58-9ba1-c3341e9a2445" xlink:to="loc_srt_SegmentGeographicalDomain_aef764dd-df2f-48f8-bbf7-1062a7691499" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_734edcee-4ce3-459f-9ad7-8d4c41fbdb8d" xlink:href="https://xbrl.sec.gov/country/2024/country-2024.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_aef764dd-df2f-48f8-bbf7-1062a7691499" xlink:to="loc_country_US_734edcee-4ce3-459f-9ad7-8d4c41fbdb8d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_DE_2f7f784e-0e5d-4817-af92-c90a2c7523b9" xlink:href="https://xbrl.sec.gov/country/2024/country-2024.xsd#country_DE"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_aef764dd-df2f-48f8-bbf7-1062a7691499" xlink:to="loc_country_DE_2f7f784e-0e5d-4817-af92-c90a2c7523b9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_RestOfTheWorldMember_ac20de22-fa8d-4a01-a25f-9885a12f07c3" xlink:href="bntx-20241231.xsd#bntx_RestOfTheWorldMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_aef764dd-df2f-48f8-bbf7-1062a7691499" xlink:to="loc_bntx_RestOfTheWorldMember_ac20de22-fa8d-4a01-a25f-9885a12f07c3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RevenueFromContractsWithCustomers_4ed1cea4-8c2c-4baf-9625-bd9b2cf4cccd" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RevenueFromContractsWithCustomers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersLineItems_452dfeb1-5e5d-48ef-af09-3d1be5c7538e" xlink:to="loc_ifrs-full_RevenueFromContractsWithCustomers_4ed1cea4-8c2c-4baf-9625-bd9b2cf4cccd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PercentageOfEntitysRevenue_5ac9282b-3a2f-475a-8fa0-ebd46c29d364" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_PercentageOfEntitysRevenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersLineItems_452dfeb1-5e5d-48ef-af09-3d1be5c7538e" xlink:to="loc_ifrs-full_PercentageOfEntitysRevenue_5ac9282b-3a2f-475a-8fa0-ebd46c29d364" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/RevenuesfromContractswithCustomersTimingofRevenueDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#RevenuesfromContractswithCustomersTimingofRevenueDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/RevenuesfromContractswithCustomersTimingofRevenueDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersLineItems_3e125004-0cf1-4c1c-8a50-3ad77f9a5a77" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable_63f9212d-fa5f-4b66-b927-307791a1e990" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersLineItems_3e125004-0cf1-4c1c-8a50-3ad77f9a5a77" xlink:to="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable_63f9212d-fa5f-4b66-b927-307791a1e990" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TimingOfTransferOfGoodsOrServicesAxis_c380d7e6-9cca-48c7-94f7-a786be7ef701" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TimingOfTransferOfGoodsOrServicesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable_63f9212d-fa5f-4b66-b927-307791a1e990" xlink:to="loc_ifrs-full_TimingOfTransferOfGoodsOrServicesAxis_c380d7e6-9cca-48c7-94f7-a786be7ef701" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TimingOfTransferOfGoodsOrServicesDomain_c380d7e6-9cca-48c7-94f7-a786be7ef701_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TimingOfTransferOfGoodsOrServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TimingOfTransferOfGoodsOrServicesAxis_c380d7e6-9cca-48c7-94f7-a786be7ef701" xlink:to="loc_ifrs-full_TimingOfTransferOfGoodsOrServicesDomain_c380d7e6-9cca-48c7-94f7-a786be7ef701_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TimingOfTransferOfGoodsOrServicesDomain_3843c2ec-9cc5-42c1-a3c1-78349ee66d67" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TimingOfTransferOfGoodsOrServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TimingOfTransferOfGoodsOrServicesAxis_c380d7e6-9cca-48c7-94f7-a786be7ef701" xlink:to="loc_ifrs-full_TimingOfTransferOfGoodsOrServicesDomain_3843c2ec-9cc5-42c1-a3c1-78349ee66d67" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GoodsOrServicesTransferredAtPointInTimeMember_26045eb7-f5d7-43f2-b41c-65ab1246d4c6" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_GoodsOrServicesTransferredAtPointInTimeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TimingOfTransferOfGoodsOrServicesDomain_3843c2ec-9cc5-42c1-a3c1-78349ee66d67" xlink:to="loc_ifrs-full_GoodsOrServicesTransferredAtPointInTimeMember_26045eb7-f5d7-43f2-b41c-65ab1246d4c6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GoodsOrServicesTransferredOverTimeMember_b2c78dff-0cd5-4c12-9781-d54251cfd800" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_GoodsOrServicesTransferredOverTimeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TimingOfTransferOfGoodsOrServicesDomain_3843c2ec-9cc5-42c1-a3c1-78349ee66d67" xlink:to="loc_ifrs-full_GoodsOrServicesTransferredOverTimeMember_b2c78dff-0cd5-4c12-9781-d54251cfd800" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_SalesBasedOrUsageBasedRoyaltyRecognitionConstraintModelMember_64a18cc0-f0db-4b11-849e-8255bea28db2" xlink:href="bntx-20241231.xsd#bntx_SalesBasedOrUsageBasedRoyaltyRecognitionConstraintModelMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TimingOfTransferOfGoodsOrServicesDomain_3843c2ec-9cc5-42c1-a3c1-78349ee66d67" xlink:to="loc_bntx_SalesBasedOrUsageBasedRoyaltyRecognitionConstraintModelMember_64a18cc0-f0db-4b11-849e-8255bea28db2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RevenueFromContractsWithCustomers_ae729314-08a5-4776-b889-399dc2a33892" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RevenueFromContractsWithCustomers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersLineItems_3e125004-0cf1-4c1c-8a50-3ad77f9a5a77" xlink:to="loc_ifrs-full_RevenueFromContractsWithCustomers_ae729314-08a5-4776-b889-399dc2a33892" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/RevenuesfromContractswithCustomersAdditionalInformationDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#RevenuesfromContractswithCustomersAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/RevenuesfromContractswithCustomersAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersLineItems_f25cd357-cc2e-4d59-89a9-b723dd2a63b3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable_e45f6e81-3135-4319-91e6-6cdeceabcb97" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersLineItems_f25cd357-cc2e-4d59-89a9-b723dd2a63b3" xlink:to="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable_e45f6e81-3135-4319-91e6-6cdeceabcb97" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MajorCustomersAxis_ea5c40ea-29d1-4bb7-a598-15c5436d51e8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MajorCustomersAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable_e45f6e81-3135-4319-91e6-6cdeceabcb97" xlink:to="loc_ifrs-full_MajorCustomersAxis_ea5c40ea-29d1-4bb7-a598-15c5436d51e8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MajorCustomersDomain_ea5c40ea-29d1-4bb7-a598-15c5436d51e8_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MajorCustomersDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_MajorCustomersAxis_ea5c40ea-29d1-4bb7-a598-15c5436d51e8" xlink:to="loc_ifrs-full_MajorCustomersDomain_ea5c40ea-29d1-4bb7-a598-15c5436d51e8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MajorCustomersDomain_f5910f47-3d2a-4f27-9a4f-2f2eb8349553" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MajorCustomersDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_MajorCustomersAxis_ea5c40ea-29d1-4bb7-a598-15c5436d51e8" xlink:to="loc_ifrs-full_MajorCustomersDomain_f5910f47-3d2a-4f27-9a4f-2f2eb8349553" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PfizerIncMember_406f3f00-6965-4458-844b-f588ccc8e7b2" xlink:href="bntx-20241231.xsd#bntx_PfizerIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MajorCustomersDomain_f5910f47-3d2a-4f27-9a4f-2f2eb8349553" xlink:to="loc_bntx_PfizerIncMember_406f3f00-6965-4458-844b-f588ccc8e7b2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ContractLiabilities_834087f2-c3bc-4543-9c9a-c3e9c670a21b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ContractLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersLineItems_f25cd357-cc2e-4d59-89a9-b723dd2a63b3" xlink:to="loc_ifrs-full_ContractLiabilities_834087f2-c3bc-4543-9c9a-c3e9c670a21b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_RemainingUpfrontFeesContractLiabilities_86d921e1-4f5f-481a-b3f6-c3a993431d7b" xlink:href="bntx-20241231.xsd#bntx_RemainingUpfrontFeesContractLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersLineItems_f25cd357-cc2e-4d59-89a9-b723dd2a63b3" xlink:to="loc_bntx_RemainingUpfrontFeesContractLiabilities_86d921e1-4f5f-481a-b3f6-c3a993431d7b" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/IncomeandExpensesAdditionalInformationDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#IncomeandExpensesAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/IncomeandExpensesAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:href="bntx-20241231.xsd#bntx_DisclosureOfIncomeAndExpensesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DisclosureOfIncomeAndExpensesTable_933d2da3-eb1e-42c1-b879-1946ac8ca05a" xlink:href="bntx-20241231.xsd#bntx_DisclosureOfIncomeAndExpensesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:to="loc_bntx_DisclosureOfIncomeAndExpensesTable_933d2da3-eb1e-42c1-b879-1946ac8ca05a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_27e520f3-a07c-4a03-9cef-5f3c92948028" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesTable_933d2da3-eb1e-42c1-b879-1946ac8ca05a" xlink:to="loc_srt_RangeAxis_27e520f3-a07c-4a03-9cef-5f3c92948028" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_27e520f3-a07c-4a03-9cef-5f3c92948028_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_27e520f3-a07c-4a03-9cef-5f3c92948028" xlink:to="loc_srt_RangeMember_27e520f3-a07c-4a03-9cef-5f3c92948028_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_0c6e0166-5ddd-4f71-a4d6-030e1c17621b" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_27e520f3-a07c-4a03-9cef-5f3c92948028" xlink:to="loc_srt_RangeMember_0c6e0166-5ddd-4f71-a4d6-030e1c17621b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_a29dad4f-2726-44b5-98c1-0ac6be354a99" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_0c6e0166-5ddd-4f71-a4d6-030e1c17621b" xlink:to="loc_srt_MaximumMember_a29dad4f-2726-44b5-98c1-0ac6be354a99" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_8ed22e56-65c1-44e1-ab3f-b971e809577e" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesTable_933d2da3-eb1e-42c1-b879-1946ac8ca05a" xlink:to="loc_srt_ProductOrServiceAxis_8ed22e56-65c1-44e1-ab3f-b971e809577e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_8ed22e56-65c1-44e1-ab3f-b971e809577e_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_8ed22e56-65c1-44e1-ab3f-b971e809577e" xlink:to="loc_srt_ProductsAndServicesDomain_8ed22e56-65c1-44e1-ab3f-b971e809577e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_d7420880-35b4-40c9-a116-2219b36688d7" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_8ed22e56-65c1-44e1-ab3f-b971e809577e" xlink:to="loc_srt_ProductsAndServicesDomain_d7420880-35b4-40c9-a116-2219b36688d7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OmicronXBB.1.5AdaptedMonovalentCOVID19VaccineMember_2f745251-5bab-44ad-885a-5d9086fac693" xlink:href="bntx-20241231.xsd#bntx_OmicronXBB.1.5AdaptedMonovalentCOVID19VaccineMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_d7420880-35b4-40c9-a116-2219b36688d7" xlink:to="loc_bntx_OmicronXBB.1.5AdaptedMonovalentCOVID19VaccineMember_2f745251-5bab-44ad-885a-5d9086fac693" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_IncreaseDecreaseCostOfSales_8c38d308-e6e7-4a6d-b734-7e38ecdcc219" xlink:href="bntx-20241231.xsd#bntx_IncreaseDecreaseCostOfSales"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:to="loc_bntx_IncreaseDecreaseCostOfSales_8c38d308-e6e7-4a6d-b734-7e38ecdcc219" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PercentageOfCostOfSales_14d855ae-925a-4700-81fe-0751307f6b6c" xlink:href="bntx-20241231.xsd#bntx_PercentageOfCostOfSales"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:to="loc_bntx_PercentageOfCostOfSales_14d855ae-925a-4700-81fe-0751307f6b6c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CostOfSales_5dbabf06-6051-4c82-9d4f-d28cad42a6c7" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CostOfSales"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:to="loc_ifrs-full_CostOfSales_5dbabf06-6051-4c82-9d4f-d28cad42a6c7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_InventoryWritedown2011_42c50eba-6abd-4c91-aa11-a9debabbbd0d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_InventoryWritedown2011"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:to="loc_ifrs-full_InventoryWritedown2011_42c50eba-6abd-4c91-aa11-a9debabbbd0d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_IncreaseDecreaseOfResearchAndDevelopmentExpense_a3c70118-ae66-4ff3-a7a4-419da717b0a8" xlink:href="bntx-20241231.xsd#bntx_IncreaseDecreaseOfResearchAndDevelopmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:to="loc_bntx_IncreaseDecreaseOfResearchAndDevelopmentExpense_a3c70118-ae66-4ff3-a7a4-419da717b0a8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PercentageOfResearchAndDevelopmentExpense_5084f35f-23a3-4658-87af-c13e7f7d163e" xlink:href="bntx-20241231.xsd#bntx_PercentageOfResearchAndDevelopmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:to="loc_bntx_PercentageOfResearchAndDevelopmentExpense_5084f35f-23a3-4658-87af-c13e7f7d163e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ResearchAndDevelopmentExpense_a305f8f8-c752-4da6-b56b-fb19cfb0c7f8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ResearchAndDevelopmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:to="loc_ifrs-full_ResearchAndDevelopmentExpense_a305f8f8-c752-4da6-b56b-fb19cfb0c7f8" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_IncreaseDecreaseOfSalesAndMarketingExpense_e05636d3-d2bc-45dd-b284-86d0b28e8c1b" xlink:href="bntx-20241231.xsd#bntx_IncreaseDecreaseOfSalesAndMarketingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:to="loc_bntx_IncreaseDecreaseOfSalesAndMarketingExpense_e05636d3-d2bc-45dd-b284-86d0b28e8c1b" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PercentageOfSalesAndMarketingExpense_a180f47d-8b7d-4fcb-9b60-ee52b9ac2054" xlink:href="bntx-20241231.xsd#bntx_PercentageOfSalesAndMarketingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:to="loc_bntx_PercentageOfSalesAndMarketingExpense_a180f47d-8b7d-4fcb-9b60-ee52b9ac2054" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SalesAndMarketingExpense_8a7a13f2-9dbe-48dc-8eae-12ca35eecb4c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SalesAndMarketingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:to="loc_ifrs-full_SalesAndMarketingExpense_8a7a13f2-9dbe-48dc-8eae-12ca35eecb4c" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_IncreaseDecreaseOfGeneralAndAdministrativeExpense_03327555-7d6e-4551-b8fb-1e88f84592f2" xlink:href="bntx-20241231.xsd#bntx_IncreaseDecreaseOfGeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:to="loc_bntx_IncreaseDecreaseOfGeneralAndAdministrativeExpense_03327555-7d6e-4551-b8fb-1e88f84592f2" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PercentageOfGeneralAndAdministrativeExpense_711d804f-676a-4c78-b034-f4bec3397f17" xlink:href="bntx-20241231.xsd#bntx_PercentageOfGeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:to="loc_bntx_PercentageOfGeneralAndAdministrativeExpense_711d804f-676a-4c78-b034-f4bec3397f17" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeneralAndAdministrativeExpense_6934d22a-f033-4285-b09e-af0d8a9e50cc" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_GeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_e7c09482-976d-4e88-8431-c8798007eefc" xlink:to="loc_ifrs-full_GeneralAndAdministrativeExpense_6934d22a-f033-4285-b09e-af0d8a9e50cc" xlink:type="arc" order="12"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/IncomeandExpensesOtherOperatingResultDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#IncomeandExpensesOtherOperatingResultDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/IncomeandExpensesOtherOperatingResultDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_dc903b81-681f-4510-b138-bc1ff01d3f96" xlink:href="bntx-20241231.xsd#bntx_DisclosureOfIncomeAndExpensesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DisclosureOfIncomeAndExpensesTable_b0012946-79b7-40b5-87ce-9476998ff1bc" xlink:href="bntx-20241231.xsd#bntx_DisclosureOfIncomeAndExpensesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_dc903b81-681f-4510-b138-bc1ff01d3f96" xlink:to="loc_bntx_DisclosureOfIncomeAndExpensesTable_b0012946-79b7-40b5-87ce-9476998ff1bc" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_IncomeAndExpensesByNatureAxis_aa4b2e1a-cd78-40e5-8ff2-742c2968ab60" xlink:href="bntx-20241231.xsd#bntx_IncomeAndExpensesByNatureAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesTable_b0012946-79b7-40b5-87ce-9476998ff1bc" xlink:to="loc_bntx_IncomeAndExpensesByNatureAxis_aa4b2e1a-cd78-40e5-8ff2-742c2968ab60" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_IncomeAndExpensesByNatureDomain_aa4b2e1a-cd78-40e5-8ff2-742c2968ab60_default" xlink:href="bntx-20241231.xsd#bntx_IncomeAndExpensesByNatureDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_bntx_IncomeAndExpensesByNatureAxis_aa4b2e1a-cd78-40e5-8ff2-742c2968ab60" xlink:to="loc_bntx_IncomeAndExpensesByNatureDomain_aa4b2e1a-cd78-40e5-8ff2-742c2968ab60_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_IncomeAndExpensesByNatureDomain_a7594f0a-7a57-4969-855e-836e9d582457" xlink:href="bntx-20241231.xsd#bntx_IncomeAndExpensesByNatureDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_bntx_IncomeAndExpensesByNatureAxis_aa4b2e1a-cd78-40e5-8ff2-742c2968ab60" xlink:to="loc_bntx_IncomeAndExpensesByNatureDomain_a7594f0a-7a57-4969-855e-836e9d582457" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_GainOnDerivativeInstrumentsAtFairValueThroughProfitOrLossMember_c246e6bb-10c0-4d77-b038-bce4742265fb" xlink:href="bntx-20241231.xsd#bntx_GainOnDerivativeInstrumentsAtFairValueThroughProfitOrLossMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_IncomeAndExpensesByNatureDomain_a7594f0a-7a57-4969-855e-836e9d582457" xlink:to="loc_bntx_GainOnDerivativeInstrumentsAtFairValueThroughProfitOrLossMember_c246e6bb-10c0-4d77-b038-bce4742265fb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_GovernmentGrantsMember_382fda4e-f61c-4245-b00c-57eaf7b8316d" xlink:href="bntx-20241231.xsd#bntx_GovernmentGrantsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_IncomeAndExpensesByNatureDomain_a7594f0a-7a57-4969-855e-836e9d582457" xlink:to="loc_bntx_GovernmentGrantsMember_382fda4e-f61c-4245-b00c-57eaf7b8316d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ForeignExchangeDifferencesMember_1408a294-874f-4c46-8aac-791205cc81db" xlink:href="bntx-20241231.xsd#bntx_ForeignExchangeDifferencesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_IncomeAndExpensesByNatureDomain_a7594f0a-7a57-4969-855e-836e9d582457" xlink:to="loc_bntx_ForeignExchangeDifferencesMember_1408a294-874f-4c46-8aac-791205cc81db" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OtherIncomeExpenseMember_47e7b965-f187-44f4-9fa2-0937d252057c" xlink:href="bntx-20241231.xsd#bntx_OtherIncomeExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_IncomeAndExpensesByNatureDomain_a7594f0a-7a57-4969-855e-836e9d582457" xlink:to="loc_bntx_OtherIncomeExpenseMember_47e7b965-f187-44f4-9fa2-0937d252057c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ContractualDisputesAndSettlementsMember_a4e1540c-b48c-4f96-a6de-86a315b14876" xlink:href="bntx-20241231.xsd#bntx_ContractualDisputesAndSettlementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_IncomeAndExpensesByNatureDomain_a7594f0a-7a57-4969-855e-836e9d582457" xlink:to="loc_bntx_ContractualDisputesAndSettlementsMember_a4e1540c-b48c-4f96-a6de-86a315b14876" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_LitigationCostsMember_505b4035-4d9e-4fd6-8102-ed1f737087f5" xlink:href="bntx-20241231.xsd#bntx_LitigationCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_IncomeAndExpensesByNatureDomain_a7594f0a-7a57-4969-855e-836e9d582457" xlink:to="loc_bntx_LitigationCostsMember_505b4035-4d9e-4fd6-8102-ed1f737087f5" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_LossOnDerivativeInstrumentsAtFairValueMember_41af0bde-c3e7-4895-af03-c2ebb82e5a9f" xlink:href="bntx-20241231.xsd#bntx_LossOnDerivativeInstrumentsAtFairValueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_IncomeAndExpensesByNatureDomain_a7594f0a-7a57-4969-855e-836e9d582457" xlink:to="loc_bntx_LossOnDerivativeInstrumentsAtFairValueMember_41af0bde-c3e7-4895-af03-c2ebb82e5a9f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MiscellaneousOtherOperatingIncome_bc72cc36-bea2-43d5-a155-ce6a7e48510d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MiscellaneousOtherOperatingIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_dc903b81-681f-4510-b138-bc1ff01d3f96" xlink:to="loc_ifrs-full_MiscellaneousOtherOperatingIncome_bc72cc36-bea2-43d5-a155-ce6a7e48510d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MiscellaneousOtherOperatingExpense_a6de462d-54d5-4243-91d4-9559ff2d734c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MiscellaneousOtherOperatingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_dc903b81-681f-4510-b138-bc1ff01d3f96" xlink:to="loc_ifrs-full_MiscellaneousOtherOperatingExpense_a6de462d-54d5-4243-91d4-9559ff2d734c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherOperatingIncomeExpense_131f4701-1f2b-4cf4-928d-972cad469eac" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_OtherOperatingIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_dc903b81-681f-4510-b138-bc1ff01d3f96" xlink:to="loc_ifrs-full_OtherOperatingIncomeExpense_131f4701-1f2b-4cf4-928d-972cad469eac" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/IncomeTaxAdditionalInformationDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#IncomeTaxAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/IncomeTaxAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_f0b7e7db-b253-434b-a64a-7ae62ddf3b6a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsTable_3aa8b91e-4d7f-4926-98cb-e166ad39902b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_f0b7e7db-b253-434b-a64a-7ae62ddf3b6a" xlink:to="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsTable_3aa8b91e-4d7f-4926-98cb-e166ad39902b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_7810c1e5-dda5-4325-980a-912d38a42604" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsTable_3aa8b91e-4d7f-4926-98cb-e166ad39902b" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_7810c1e5-dda5-4325-980a-912d38a42604" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_7810c1e5-dda5-4325-980a-912d38a42604_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_7810c1e5-dda5-4325-980a-912d38a42604" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_7810c1e5-dda5-4325-980a-912d38a42604_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_ac32984e-eecd-4078-879b-68c891508a24" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_7810c1e5-dda5-4325-980a-912d38a42604" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_ac32984e-eecd-4078-879b-68c891508a24" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ChiefExecutiveOfficerGrantMember_0593422b-bd25-41a4-b042-4777bb1e021c" xlink:href="bntx-20241231.xsd#bntx_ChiefExecutiveOfficerGrantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_ac32984e-eecd-4078-879b-68c891508a24" xlink:to="loc_bntx_ChiefExecutiveOfficerGrantMember_0593422b-bd25-41a4-b042-4777bb1e021c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_df7e5781-b4c0-495b-a170-88a141615a0f" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsTable_3aa8b91e-4d7f-4926-98cb-e166ad39902b" xlink:to="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_df7e5781-b4c0-495b-a170-88a141615a0f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountMember_df7e5781-b4c0-495b-a170-88a141615a0f_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_df7e5781-b4c0-495b-a170-88a141615a0f" xlink:to="loc_ifrs-full_CarryingAmountMember_df7e5781-b4c0-495b-a170-88a141615a0f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountMember_96fe49bd-14e9-4606-80ce-bd8ae4153f86" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_df7e5781-b4c0-495b-a170-88a141615a0f" xlink:to="loc_ifrs-full_CarryingAmountMember_96fe49bd-14e9-4606-80ce-bd8ae4153f86" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ValuationAdjustmentMember_ac9f95c0-995f-492f-a8bc-56b2e4bdb287" xlink:href="bntx-20241231.xsd#bntx_ValuationAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CarryingAmountMember_96fe49bd-14e9-4606-80ce-bd8ae4153f86" xlink:to="loc_bntx_ValuationAdjustmentMember_ac9f95c0-995f-492f-a8bc-56b2e4bdb287" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_6c7eb541-1b59-466e-9a76-02a5d8a8a073" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsTable_3aa8b91e-4d7f-4926-98cb-e166ad39902b" xlink:to="loc_srt_StatementGeographicalAxis_6c7eb541-1b59-466e-9a76-02a5d8a8a073" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_6c7eb541-1b59-466e-9a76-02a5d8a8a073_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_6c7eb541-1b59-466e-9a76-02a5d8a8a073" xlink:to="loc_srt_SegmentGeographicalDomain_6c7eb541-1b59-466e-9a76-02a5d8a8a073_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_37b41a9d-e1b1-415a-9319-73d0b7356d43" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_6c7eb541-1b59-466e-9a76-02a5d8a8a073" xlink:to="loc_srt_SegmentGeographicalDomain_37b41a9d-e1b1-415a-9319-73d0b7356d43" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_AT_cf3e77bf-d60e-46a4-916a-5cf9c51ee01e" xlink:href="https://xbrl.sec.gov/country/2024/country-2024.xsd#country_AT"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_37b41a9d-e1b1-415a-9319-73d0b7356d43" xlink:to="loc_country_AT_cf3e77bf-d60e-46a4-916a-5cf9c51ee01e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_f490bf12-f247-4aba-b99c-32afb61851e0" xlink:href="https://xbrl.sec.gov/country/2024/country-2024.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_37b41a9d-e1b1-415a-9319-73d0b7356d43" xlink:to="loc_country_US_f490bf12-f247-4aba-b99c-32afb61851e0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis_4fb6ebc3-2293-4849-823b-787e84b28958" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsTable_3aa8b91e-4d7f-4926-98cb-e166ad39902b" xlink:to="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis_4fb6ebc3-2293-4849-823b-787e84b28958" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_4fb6ebc3-2293-4849-823b-787e84b28958_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis_4fb6ebc3-2293-4849-823b-787e84b28958" xlink:to="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_4fb6ebc3-2293-4849-823b-787e84b28958_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_83b14fe5-05a6-4055-99e8-9293412ec694" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis_4fb6ebc3-2293-4849-823b-787e84b28958" xlink:to="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_83b14fe5-05a6-4055-99e8-9293412ec694" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DeductibleTemporaryDifferencesUnusedTaxLossesAndUnusedTaxCreditsMember_a9aa10b1-4475-49d9-9281-62c101802dea" xlink:href="bntx-20241231.xsd#bntx_DeductibleTemporaryDifferencesUnusedTaxLossesAndUnusedTaxCreditsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_83b14fe5-05a6-4055-99e8-9293412ec694" xlink:to="loc_bntx_DeductibleTemporaryDifferencesUnusedTaxLossesAndUnusedTaxCreditsMember_a9aa10b1-4475-49d9-9281-62c101802dea" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_UnusedTaxLossesMember_31438a2f-de66-4ce8-b05a-1b3d96522389" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_UnusedTaxLossesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_83b14fe5-05a6-4055-99e8-9293412ec694" xlink:to="loc_ifrs-full_UnusedTaxLossesMember_31438a2f-de66-4ce8-b05a-1b3d96522389" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_UnusedTaxCreditsMember_4ceb9427-b813-4df2-8b9a-3c541faeb79b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_UnusedTaxCreditsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_83b14fe5-05a6-4055-99e8-9293412ec694" xlink:to="loc_ifrs-full_UnusedTaxCreditsMember_4ceb9427-b813-4df2-8b9a-3c541faeb79b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CorporateTaxRate_5d820cd6-f246-4bfb-8b3b-a22a27e2a6db" xlink:href="bntx-20241231.xsd#bntx_CorporateTaxRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_f0b7e7db-b253-434b-a64a-7ae62ddf3b6a" xlink:to="loc_bntx_CorporateTaxRate_5d820cd6-f246-4bfb-8b3b-a22a27e2a6db" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_SolidaritySurchargeRate_b8701792-6d23-4f61-b0c8-8f4696b2c9b5" xlink:href="bntx-20241231.xsd#bntx_SolidaritySurchargeRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_f0b7e7db-b253-434b-a64a-7ae62ddf3b6a" xlink:to="loc_bntx_SolidaritySurchargeRate_b8701792-6d23-4f61-b0c8-8f4696b2c9b5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ApplicableTaxRate_9eb640ba-3c5a-4fff-9e33-2644c302d4bc" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ApplicableTaxRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_f0b7e7db-b253-434b-a64a-7ae62ddf3b6a" xlink:to="loc_ifrs-full_ApplicableTaxRate_9eb640ba-3c5a-4fff-9e33-2644c302d4bc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_TaxRateOnDeferredTaxes_026a4e70-2b99-47e2-88ba-56a0d7768a36" xlink:href="bntx-20241231.xsd#bntx_TaxRateOnDeferredTaxes"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_f0b7e7db-b253-434b-a64a-7ae62ddf3b6a" xlink:to="loc_bntx_TaxRateOnDeferredTaxes_026a4e70-2b99-47e2-88ba-56a0d7768a36" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_FederalCorporateIncomeTax_0f09ee84-c2b5-4fbc-96b2-9d600d14c039" xlink:href="bntx-20241231.xsd#bntx_FederalCorporateIncomeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_f0b7e7db-b253-434b-a64a-7ae62ddf3b6a" xlink:to="loc_bntx_FederalCorporateIncomeTax_0f09ee84-c2b5-4fbc-96b2-9d600d14c039" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AverageEffectiveTaxRate_ce445644-0abd-40c4-bbeb-f7a136d94644" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AverageEffectiveTaxRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_f0b7e7db-b253-434b-a64a-7ae62ddf3b6a" xlink:to="loc_ifrs-full_AverageEffectiveTaxRate_ce445644-0abd-40c4-bbeb-f7a136d94644" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DeferredTaxAssets_f8588017-e2e0-438e-8469-c0662f55e2df" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DeferredTaxAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_f0b7e7db-b253-434b-a64a-7ae62ddf3b6a" xlink:to="loc_ifrs-full_DeferredTaxAssets_f8588017-e2e0-438e-8469-c0662f55e2df" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DeferredTaxLiabilityAsset_bb813148-9793-4cf5-af68-ee72c3ddddfa" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DeferredTaxLiabilityAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_f0b7e7db-b253-434b-a64a-7ae62ddf3b6a" xlink:to="loc_ifrs-full_DeferredTaxLiabilityAsset_bb813148-9793-4cf5-af68-ee72c3ddddfa" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TemporaryDifferencesAssociatedWithInvestmentsInSubsidiariesBranchesAndAssociatesAndInterestsInJointVentures_fd4fcb9b-c4bf-45ef-acfe-d8cc5348173c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TemporaryDifferencesAssociatedWithInvestmentsInSubsidiariesBranchesAndAssociatesAndInterestsInJointVentures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_f0b7e7db-b253-434b-a64a-7ae62ddf3b6a" xlink:to="loc_ifrs-full_TemporaryDifferencesAssociatedWithInvestmentsInSubsidiariesBranchesAndAssociatesAndInterestsInJointVentures_fd4fcb9b-c4bf-45ef-acfe-d8cc5348173c" xlink:type="arc" order="8"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/IncomeTaxSummaryofDeferredTaxesDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#IncomeTaxSummaryofDeferredTaxesDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/IncomeTaxSummaryofDeferredTaxesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_376929af-078d-41e8-9f32-5c586e9d931e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsTable_397dbbfb-3ffd-4a67-9ec3-0b6056e4f2a0" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_376929af-078d-41e8-9f32-5c586e9d931e" xlink:to="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsTable_397dbbfb-3ffd-4a67-9ec3-0b6056e4f2a0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis_e1ec12e7-a268-42e2-bb87-704f7e123dcf" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsTable_397dbbfb-3ffd-4a67-9ec3-0b6056e4f2a0" xlink:to="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis_e1ec12e7-a268-42e2-bb87-704f7e123dcf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_e1ec12e7-a268-42e2-bb87-704f7e123dcf_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis_e1ec12e7-a268-42e2-bb87-704f7e123dcf" xlink:to="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_e1ec12e7-a268-42e2-bb87-704f7e123dcf_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis_e1ec12e7-a268-42e2-bb87-704f7e123dcf" xlink:to="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_FixedAssetsMember_f2d2d7d3-badf-4dd6-a50b-a655fe084a80" xlink:href="bntx-20241231.xsd#bntx_FixedAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:to="loc_bntx_FixedAssetsMember_f2d2d7d3-badf-4dd6-a50b-a655fe084a80" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RightofuseAssetsMember_fe7fcee5-2a97-4d64-96bd-c5e848c294f5" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RightofuseAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:to="loc_ifrs-full_RightofuseAssetsMember_fe7fcee5-2a97-4d64-96bd-c5e848c294f5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_Inventories1Member_38435c32-4ae8-4abd-a9e8-2e0f5b5c3b50" xlink:href="bntx-20241231.xsd#bntx_Inventories1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:to="loc_bntx_Inventories1Member_38435c32-4ae8-4abd-a9e8-2e0f5b5c3b50" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_TradeAndOtherReceivablesMember_e4fb49dd-c7f8-4d63-bd71-b9a6881a9e0b" xlink:href="bntx-20241231.xsd#bntx_TradeAndOtherReceivablesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:to="loc_bntx_TradeAndOtherReceivablesMember_e4fb49dd-c7f8-4d63-bd71-b9a6881a9e0b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LeaseLiabilitiesMember_741877a9-01a8-4f20-851b-578916c898e6" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LeaseLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:to="loc_ifrs-full_LeaseLiabilitiesMember_741877a9-01a8-4f20-851b-578916c898e6" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_LoansAndBorrowingsMember_c52546c3-d501-4a3d-b258-65f2c0cac19d" xlink:href="bntx-20241231.xsd#bntx_LoansAndBorrowingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:to="loc_bntx_LoansAndBorrowingsMember_c52546c3-d501-4a3d-b258-65f2c0cac19d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ContractLiabilitiesMember_d7b04b80-60f5-4287-a104-69b00d6f0d74" xlink:href="bntx-20241231.xsd#bntx_ContractLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:to="loc_bntx_ContractLiabilitiesMember_d7b04b80-60f5-4287-a104-69b00d6f0d74" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NetEmployeeDefinedBenefitLiabilitiesMember_f59a0df2-8b63-48fd-8a64-bfe0d4e6c63a" xlink:href="bntx-20241231.xsd#bntx_NetEmployeeDefinedBenefitLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:to="loc_bntx_NetEmployeeDefinedBenefitLiabilitiesMember_f59a0df2-8b63-48fd-8a64-bfe0d4e6c63a" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareBasedPaymentArrangementsMember_29f0bb08-e58b-4916-b7d8-3edabc993360" xlink:href="bntx-20241231.xsd#bntx_ShareBasedPaymentArrangementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:to="loc_bntx_ShareBasedPaymentArrangementsMember_29f0bb08-e58b-4916-b7d8-3edabc993360" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OtherProvisionsMember_ff1fe3e1-abd2-4583-901a-e843728107f3" xlink:href="bntx-20241231.xsd#bntx_OtherProvisionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:to="loc_bntx_OtherProvisionsMember_ff1fe3e1-abd2-4583-901a-e843728107f3" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OtherDeferredTaxesMember_a0f04306-06bc-4c3f-8786-e3452de926e6" xlink:href="bntx-20241231.xsd#bntx_OtherDeferredTaxesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:to="loc_bntx_OtherDeferredTaxesMember_a0f04306-06bc-4c3f-8786-e3452de926e6" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_UnusedTaxCreditsMember_a105beca-118a-45ae-9a9a-4a99f405021c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_UnusedTaxCreditsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:to="loc_ifrs-full_UnusedTaxCreditsMember_a105beca-118a-45ae-9a9a-4a99f405021c" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ThereofDeferredTaxAssetsMember_46003bc7-c513-44f0-a315-0c17789d9acc" xlink:href="bntx-20241231.xsd#bntx_ThereofDeferredTaxAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:to="loc_bntx_ThereofDeferredTaxAssetsMember_46003bc7-c513-44f0-a315-0c17789d9acc" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ThereofDeferredTaxLiabilityMember_9fadb743-ae9c-4b58-abb9-3183229515eb" xlink:href="bntx-20241231.xsd#bntx_ThereofDeferredTaxLiabilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_5b1c758a-1ae9-454b-aded-ca115f29cf4d" xlink:to="loc_bntx_ThereofDeferredTaxLiabilityMember_9fadb743-ae9c-4b58-abb9-3183229515eb" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_e45a913f-1592-41e8-8ea6-06aec51838b6" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsTable_397dbbfb-3ffd-4a67-9ec3-0b6056e4f2a0" xlink:to="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_e45a913f-1592-41e8-8ea6-06aec51838b6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountMember_e45a913f-1592-41e8-8ea6-06aec51838b6_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_e45a913f-1592-41e8-8ea6-06aec51838b6" xlink:to="loc_ifrs-full_CarryingAmountMember_e45a913f-1592-41e8-8ea6-06aec51838b6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountMember_7c31f9b3-e4f7-4fe0-b0b1-31d48e7b1edc" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_e45a913f-1592-41e8-8ea6-06aec51838b6" xlink:to="loc_ifrs-full_CarryingAmountMember_7c31f9b3-e4f7-4fe0-b0b1-31d48e7b1edc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GrossCarryingAmountMember_f699c8d0-6351-400c-b024-572ca1daaee4" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_GrossCarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CarryingAmountMember_7c31f9b3-e4f7-4fe0-b0b1-31d48e7b1edc" xlink:to="loc_ifrs-full_GrossCarryingAmountMember_f699c8d0-6351-400c-b024-572ca1daaee4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ValuationAdjustmentMember_227ab1b6-56e5-4a77-8514-69f48081e9bd" xlink:href="bntx-20241231.xsd#bntx_ValuationAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CarryingAmountMember_7c31f9b3-e4f7-4fe0-b0b1-31d48e7b1edc" xlink:to="loc_bntx_ValuationAdjustmentMember_227ab1b6-56e5-4a77-8514-69f48081e9bd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DeferredTaxLiabilityAsset_42d8baeb-3670-443f-81fd-e5fc9048c55c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DeferredTaxLiabilityAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_376929af-078d-41e8-9f32-5c586e9d931e" xlink:to="loc_ifrs-full_DeferredTaxLiabilityAsset_42d8baeb-3670-443f-81fd-e5fc9048c55c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DeferredTaxExpenseIncomeRecognisedInProfitOrLoss_0e2d14f0-afc9-4133-b64c-dbad34664545" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DeferredTaxExpenseIncomeRecognisedInProfitOrLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_376929af-078d-41e8-9f32-5c586e9d931e" xlink:to="loc_ifrs-full_DeferredTaxExpenseIncomeRecognisedInProfitOrLoss_0e2d14f0-afc9-4133-b64c-dbad34664545" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DeferredTaxExpenseIncomeRecognisedInOtherComprehensiveIncome_9660255e-40fb-45a0-898e-f9603b749fc3" xlink:href="bntx-20241231.xsd#bntx_DeferredTaxExpenseIncomeRecognisedInOtherComprehensiveIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_376929af-078d-41e8-9f32-5c586e9d931e" xlink:to="loc_bntx_DeferredTaxExpenseIncomeRecognisedInOtherComprehensiveIncome_9660255e-40fb-45a0-898e-f9603b749fc3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DeferredTaxRelatingToItemsChargedOrCreditedDirectlyToEquity_d9ab6399-f8d3-47d1-89b3-da36065526a9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DeferredTaxRelatingToItemsChargedOrCreditedDirectlyToEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_376929af-078d-41e8-9f32-5c586e9d931e" xlink:to="loc_ifrs-full_DeferredTaxRelatingToItemsChargedOrCreditedDirectlyToEquity_d9ab6399-f8d3-47d1-89b3-da36065526a9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughBusinessCombinationsDeferredTaxLiabilityAsset_aab31fb9-9617-4400-b7a3-ef33103215eb" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IncreaseDecreaseThroughBusinessCombinationsDeferredTaxLiabilityAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsLineItems_376929af-078d-41e8-9f32-5c586e9d931e" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughBusinessCombinationsDeferredTaxLiabilityAsset_aab31fb9-9617-4400-b7a3-ef33103215eb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DeferredTaxLiabilityAsset_968d56eb-fb9b-49e0-af70-099823b3eabd" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DeferredTaxLiabilityAsset"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/OtherIntangibleAssetsandGoodwillGoodwillDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#OtherIntangibleAssetsandGoodwillGoodwillDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/OtherIntangibleAssetsandGoodwillGoodwillDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_722d7798-3b95-4d4d-8478-8b115207c78c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillTable_25f0fc63-5a23-4490-b0f7-220384b3f4da" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_722d7798-3b95-4d4d-8478-8b115207c78c" xlink:to="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillTable_25f0fc63-5a23-4490-b0f7-220384b3f4da" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_ac51732c-8d0d-4734-a9da-5c2d70bfb4da" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillTable_25f0fc63-5a23-4490-b0f7-220384b3f4da" xlink:to="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_ac51732c-8d0d-4734-a9da-5c2d70bfb4da" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountMember_ac51732c-8d0d-4734-a9da-5c2d70bfb4da_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_ac51732c-8d0d-4734-a9da-5c2d70bfb4da" xlink:to="loc_ifrs-full_CarryingAmountMember_ac51732c-8d0d-4734-a9da-5c2d70bfb4da_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountMember_e60e78b1-f734-4086-9371-ebd6e22305b2" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_ac51732c-8d0d-4734-a9da-5c2d70bfb4da" xlink:to="loc_ifrs-full_CarryingAmountMember_e60e78b1-f734-4086-9371-ebd6e22305b2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GrossCarryingAmountMember_b3b30dad-3e1a-470f-ab47-3f6e6779c400" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_GrossCarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CarryingAmountMember_e60e78b1-f734-4086-9371-ebd6e22305b2" xlink:to="loc_ifrs-full_GrossCarryingAmountMember_b3b30dad-3e1a-470f-ab47-3f6e6779c400" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis_15e4fedd-2530-418b-a6aa-2bfb0a688ace" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillTable_25f0fc63-5a23-4490-b0f7-220384b3f4da" xlink:to="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis_15e4fedd-2530-418b-a6aa-2bfb0a688ace" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_15e4fedd-2530-418b-a6aa-2bfb0a688ace_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis_15e4fedd-2530-418b-a6aa-2bfb0a688ace" xlink:to="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_15e4fedd-2530-418b-a6aa-2bfb0a688ace_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_606e1fb6-587e-4704-ba8a-2974a7b1f77d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis_15e4fedd-2530-418b-a6aa-2bfb0a688ace" xlink:to="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_606e1fb6-587e-4704-ba8a-2974a7b1f77d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GoodwillMember_5fe276dc-7dde-406d-a83e-fa7aacb820b1" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_GoodwillMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_606e1fb6-587e-4704-ba8a-2974a7b1f77d" xlink:to="loc_ifrs-full_GoodwillMember_5fe276dc-7dde-406d-a83e-fa7aacb820b1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IntangibleAssetsAndGoodwill_e4960f33-c591-4cc0-8dc1-b2814c66c6ef" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IntangibleAssetsAndGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_722d7798-3b95-4d4d-8478-8b115207c78c" xlink:to="loc_ifrs-full_IntangibleAssetsAndGoodwill_e4960f33-c591-4cc0-8dc1-b2814c66c6ef" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesIntangibleAssetsAndGoodwill_a92f72a1-7083-4025-b7e2-e4e21d039b02" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesIntangibleAssetsAndGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_722d7798-3b95-4d4d-8478-8b115207c78c" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesIntangibleAssetsAndGoodwill_a92f72a1-7083-4025-b7e2-e4e21d039b02" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AcquisitionsThroughBusinessCombinationsIntangibleAssetsAndGoodwill_0e11577a-dacd-42fc-ad9f-81dc99654978" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AcquisitionsThroughBusinessCombinationsIntangibleAssetsAndGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_722d7798-3b95-4d4d-8478-8b115207c78c" xlink:to="loc_ifrs-full_AcquisitionsThroughBusinessCombinationsIntangibleAssetsAndGoodwill_0e11577a-dacd-42fc-ad9f-81dc99654978" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IntangibleAssetsAndGoodwill_903077c3-1254-40ad-adfa-a23f123f2ab7" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IntangibleAssetsAndGoodwill"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/OtherIntangibleAssetsandGoodwillSummaryofGoodwillRecognizedDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#OtherIntangibleAssetsandGoodwillSummaryofGoodwillRecognizedDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/OtherIntangibleAssetsandGoodwillSummaryofGoodwillRecognizedDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_75550fd7-173a-4d79-ba29-432dff76d4bf" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfIntangibleAssetsTable_a5bb7a03-f120-42fa-b8b4-279e98b59fb9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_75550fd7-173a-4d79-ba29-432dff76d4bf" xlink:to="loc_ifrs-full_DisclosureOfIntangibleAssetsTable_a5bb7a03-f120-42fa-b8b4-279e98b59fb9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_8d92d314-fb04-4399-b36e-1f0d95701dba" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ConsolidatedStructuredEntitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsTable_a5bb7a03-f120-42fa-b8b4-279e98b59fb9" xlink:to="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_8d92d314-fb04-4399-b36e-1f0d95701dba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_8d92d314-fb04-4399-b36e-1f0d95701dba_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ConsolidatedStructuredEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_8d92d314-fb04-4399-b36e-1f0d95701dba" xlink:to="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_8d92d314-fb04-4399-b36e-1f0d95701dba_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_e2b233c1-ed0c-4e9d-8820-2280d3de62a5" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ConsolidatedStructuredEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_8d92d314-fb04-4399-b36e-1f0d95701dba" xlink:to="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_e2b233c1-ed0c-4e9d-8820-2280d3de62a5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CGUImmunotherapiesMember_150c8c57-ee6c-491b-a087-8cbdf4a2d07c" xlink:href="bntx-20241231.xsd#bntx_CGUImmunotherapiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_e2b233c1-ed0c-4e9d-8820-2280d3de62a5" xlink:to="loc_bntx_CGUImmunotherapiesMember_150c8c57-ee6c-491b-a087-8cbdf4a2d07c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ExternalProductSalesToJPTMember_bc191388-a6eb-44fd-b042-64ef8e47bc6c" xlink:href="bntx-20241231.xsd#bntx_ExternalProductSalesToJPTMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_e2b233c1-ed0c-4e9d-8820-2280d3de62a5" xlink:to="loc_bntx_ExternalProductSalesToJPTMember_bc191388-a6eb-44fd-b042-64ef8e47bc6c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ExternalBusinessOfInstaDeepMember_cf18927c-d92c-40d6-9031-481b43e08db5" xlink:href="bntx-20241231.xsd#bntx_ExternalBusinessOfInstaDeepMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_e2b233c1-ed0c-4e9d-8820-2280d3de62a5" xlink:to="loc_bntx_ExternalBusinessOfInstaDeepMember_cf18927c-d92c-40d6-9031-481b43e08db5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Goodwill_79fb94a5-41b7-4a66-9bca-0c5f105e71b7" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_75550fd7-173a-4d79-ba29-432dff76d4bf" xlink:to="loc_ifrs-full_Goodwill_79fb94a5-41b7-4a66-9bca-0c5f105e71b7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IntangibleAssetsWithIndefiniteUsefulLife_bd43efce-ba7f-4d14-8a29-7750c45c47cf" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IntangibleAssetsWithIndefiniteUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_75550fd7-173a-4d79-ba29-432dff76d4bf" xlink:to="loc_ifrs-full_IntangibleAssetsWithIndefiniteUsefulLife_bd43efce-ba7f-4d14-8a29-7750c45c47cf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_IntangibleAssetsWithIndefiniteUsefulLifeAndGoodwill_2a7269cc-e103-4ebf-86d2-2afdf5315562" xlink:href="bntx-20241231.xsd#bntx_IntangibleAssetsWithIndefiniteUsefulLifeAndGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_75550fd7-173a-4d79-ba29-432dff76d4bf" xlink:to="loc_bntx_IntangibleAssetsWithIndefiniteUsefulLifeAndGoodwill_2a7269cc-e103-4ebf-86d2-2afdf5315562" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/OtherIntangibleAssetsandGoodwillAdditionalInformationDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#OtherIntangibleAssetsandGoodwillAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/OtherIntangibleAssetsandGoodwillAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_97e5f9d2-9f80-4454-b603-dd31f71d807e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfIntangibleAssetsTable_e1e97241-fa4a-4dbd-87c1-ad6f0abf9e16" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_97e5f9d2-9f80-4454-b603-dd31f71d807e" xlink:to="loc_ifrs-full_DisclosureOfIntangibleAssetsTable_e1e97241-fa4a-4dbd-87c1-ad6f0abf9e16" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInAssociatesAxis_8e38bc24-99e3-47af-a1cb-ab0a27d5b068" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SignificantInvestmentsInAssociatesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsTable_e1e97241-fa4a-4dbd-87c1-ad6f0abf9e16" xlink:to="loc_ifrs-full_SignificantInvestmentsInAssociatesAxis_8e38bc24-99e3-47af-a1cb-ab0a27d5b068" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_8e38bc24-99e3-47af-a1cb-ab0a27d5b068_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SignificantInvestmentsInAssociatesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesAxis_8e38bc24-99e3-47af-a1cb-ab0a27d5b068" xlink:to="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_8e38bc24-99e3-47af-a1cb-ab0a27d5b068_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_3b456a23-631c-4092-b3b7-6ba45be841c1" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SignificantInvestmentsInAssociatesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesAxis_8e38bc24-99e3-47af-a1cb-ab0a27d5b068" xlink:to="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_3b456a23-631c-4092-b3b7-6ba45be841c1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DualityBiologicsSuzhouCoLtdMember_671c5cf4-0bbe-4d5d-bf9d-ce7543a852ee" xlink:href="bntx-20241231.xsd#bntx_DualityBiologicsSuzhouCoLtdMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_3b456a23-631c-4092-b3b7-6ba45be841c1" xlink:to="loc_bntx_DualityBiologicsSuzhouCoLtdMember_671c5cf4-0bbe-4d5d-bf9d-ce7543a852ee" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OncoC4IncMember_167abf73-e242-4475-98b5-07401ec9af03" xlink:href="bntx-20241231.xsd#bntx_OncoC4IncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_3b456a23-631c-4092-b3b7-6ba45be841c1" xlink:to="loc_bntx_OncoC4IncMember_167abf73-e242-4475-98b5-07401ec9af03" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_MedilinkTherapeuticsSuzhouCoLtdMember_d585d342-4599-44f0-8199-3dedc44a5ed2" xlink:href="bntx-20241231.xsd#bntx_MedilinkTherapeuticsSuzhouCoLtdMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_3b456a23-631c-4092-b3b7-6ba45be841c1" xlink:to="loc_bntx_MedilinkTherapeuticsSuzhouCoLtdMember_d585d342-4599-44f0-8199-3dedc44a5ed2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BiotheusIncMember_b83ea97c-2c5a-4d22-b8a3-b0c4ae9d7166" xlink:href="bntx-20241231.xsd#bntx_BiotheusIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_3b456a23-631c-4092-b3b7-6ba45be841c1" xlink:to="loc_bntx_BiotheusIncMember_b83ea97c-2c5a-4d22-b8a3-b0c4ae9d7166" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsAxis_2d9eb4c3-6b0f-4cac-8c7a-a34792606bc8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BusinessCombinationsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsTable_e1e97241-fa4a-4dbd-87c1-ad6f0abf9e16" xlink:to="loc_ifrs-full_BusinessCombinationsAxis_2d9eb4c3-6b0f-4cac-8c7a-a34792606bc8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsDomain_2d9eb4c3-6b0f-4cac-8c7a-a34792606bc8_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BusinessCombinationsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_BusinessCombinationsAxis_2d9eb4c3-6b0f-4cac-8c7a-a34792606bc8" xlink:to="loc_ifrs-full_BusinessCombinationsDomain_2d9eb4c3-6b0f-4cac-8c7a-a34792606bc8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsDomain_cc878894-1d2f-4dc8-9c7a-e1e1d6b14a72" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BusinessCombinationsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_BusinessCombinationsAxis_2d9eb4c3-6b0f-4cac-8c7a-a34792606bc8" xlink:to="loc_ifrs-full_BusinessCombinationsDomain_cc878894-1d2f-4dc8-9c7a-e1e1d6b14a72" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DeepChainTechnologyMember_435bc743-dc84-4511-a6fa-75d3ade3598d" xlink:href="bntx-20241231.xsd#bntx_DeepChainTechnologyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_BusinessCombinationsDomain_cc878894-1d2f-4dc8-9c7a-e1e1d6b14a72" xlink:to="loc_bntx_DeepChainTechnologyMember_435bc743-dc84-4511-a6fa-75d3ade3598d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis_7cb177f9-64cc-4863-b8bf-cd37ea830236" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsTable_e1e97241-fa4a-4dbd-87c1-ad6f0abf9e16" xlink:to="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis_7cb177f9-64cc-4863-b8bf-cd37ea830236" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_7cb177f9-64cc-4863-b8bf-cd37ea830236_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis_7cb177f9-64cc-4863-b8bf-cd37ea830236" xlink:to="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_7cb177f9-64cc-4863-b8bf-cd37ea830236_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_0ee7e3ec-b901-4dd9-a69f-cc9c7d6d75a7" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis_7cb177f9-64cc-4863-b8bf-cd37ea830236" xlink:to="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_0ee7e3ec-b901-4dd9-a69f-cc9c7d6d75a7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_MiscellaneousMember_415e2889-ce8b-4fc7-b602-e6d2bb4f8bb9" xlink:href="bntx-20241231.xsd#bntx_MiscellaneousMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_0ee7e3ec-b901-4dd9-a69f-cc9c7d6d75a7" xlink:to="loc_bntx_MiscellaneousMember_415e2889-ce8b-4fc7-b602-e6d2bb4f8bb9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_InProcessRDMember_f649d8bb-c5f1-4d35-a382-5ea4a4245757" xlink:href="bntx-20241231.xsd#bntx_InProcessRDMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_0ee7e3ec-b901-4dd9-a69f-cc9c7d6d75a7" xlink:to="loc_bntx_InProcessRDMember_f649d8bb-c5f1-4d35-a382-5ea4a4245757" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_bde51864-efca-4936-9a6f-49862228e0ad" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsTable_e1e97241-fa4a-4dbd-87c1-ad6f0abf9e16" xlink:to="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_bde51864-efca-4936-9a6f-49862228e0ad" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountMember_bde51864-efca-4936-9a6f-49862228e0ad_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_bde51864-efca-4936-9a6f-49862228e0ad" xlink:to="loc_ifrs-full_CarryingAmountMember_bde51864-efca-4936-9a6f-49862228e0ad_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountDomain_d2daf426-b166-4242-a1fd-5ff268658c92" xlink:href="bntx-20241231.xsd#bntx_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_bde51864-efca-4936-9a6f-49862228e0ad" xlink:to="loc_bntx_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountDomain_d2daf426-b166-4242-a1fd-5ff268658c92" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountMember_d7a1326c-e1d2-405a-9e90-218a1dd1f042" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountDomain_d2daf426-b166-4242-a1fd-5ff268658c92" xlink:to="loc_ifrs-full_CarryingAmountMember_d7a1326c-e1d2-405a-9e90-218a1dd1f042" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AccumulatedDepreciationAmortisationAndImpairmentMember_def0a370-1203-49fd-9dbf-b40cae43609e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AccumulatedDepreciationAmortisationAndImpairmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CarryingAmountMember_d7a1326c-e1d2-405a-9e90-218a1dd1f042" xlink:to="loc_ifrs-full_AccumulatedDepreciationAmortisationAndImpairmentMember_def0a370-1203-49fd-9dbf-b40cae43609e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GrossCarryingAmountMember_49d8d13f-e13b-4041-bb9b-a0a60f089e6c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_GrossCarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CarryingAmountMember_d7a1326c-e1d2-405a-9e90-218a1dd1f042" xlink:to="loc_ifrs-full_GrossCarryingAmountMember_49d8d13f-e13b-4041-bb9b-a0a60f089e6c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DescriptionOfGrowthRateUsedToExtrapolateCashFlowProjections_8b42d28c-85e8-4426-b360-628662504d9a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DescriptionOfGrowthRateUsedToExtrapolateCashFlowProjections"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_97e5f9d2-9f80-4454-b603-dd31f71d807e" xlink:to="loc_ifrs-full_DescriptionOfGrowthRateUsedToExtrapolateCashFlowProjections_8b42d28c-85e8-4426-b360-628662504d9a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IntangibleAssetsAndGoodwill_5d0eba55-ef91-4e9c-9697-3ac9d573d94b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IntangibleAssetsAndGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_97e5f9d2-9f80-4454-b603-dd31f71d807e" xlink:to="loc_ifrs-full_IntangibleAssetsAndGoodwill_5d0eba55-ef91-4e9c-9697-3ac9d573d94b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ImpairmentLossRecognisedInProfitOrLossIntangibleAssetsAndGoodwill_406ab3ac-bbaa-40b8-9a1a-0a40dc07636b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ImpairmentLossRecognisedInProfitOrLossIntangibleAssetsAndGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_97e5f9d2-9f80-4454-b603-dd31f71d807e" xlink:to="loc_ifrs-full_ImpairmentLossRecognisedInProfitOrLossIntangibleAssetsAndGoodwill_406ab3ac-bbaa-40b8-9a1a-0a40dc07636b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PercentageOfImpairmentLossForAReductionOfFutureCashFlows_ff386c0e-760f-4d6c-8723-a414c833c33e" xlink:href="bntx-20241231.xsd#bntx_PercentageOfImpairmentLossForAReductionOfFutureCashFlows"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_97e5f9d2-9f80-4454-b603-dd31f71d807e" xlink:to="loc_bntx_PercentageOfImpairmentLossForAReductionOfFutureCashFlows_ff386c0e-760f-4d6c-8723-a414c833c33e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CapitalisationRateOfBorrowingCostsEligibleForCapitalisation_adad23c7-c52c-4aa7-ac77-d294022163fe" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CapitalisationRateOfBorrowingCostsEligibleForCapitalisation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_97e5f9d2-9f80-4454-b603-dd31f71d807e" xlink:to="loc_ifrs-full_CapitalisationRateOfBorrowingCostsEligibleForCapitalisation_adad23c7-c52c-4aa7-ac77-d294022163fe" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseInIntangibleAssetsAndGoodwill_7b0e93b8-8ae7-4029-9494-96577379491b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IncreaseDecreaseInIntangibleAssetsAndGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_97e5f9d2-9f80-4454-b603-dd31f71d807e" xlink:to="loc_ifrs-full_IncreaseDecreaseInIntangibleAssetsAndGoodwill_7b0e93b8-8ae7-4029-9494-96577379491b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdditionsOtherThanThroughBusinessCombinationsIntangibleAssetsOtherThanGoodwill_3e514687-89c6-4e47-ad78-d4c722adccf0" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AdditionsOtherThanThroughBusinessCombinationsIntangibleAssetsOtherThanGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_97e5f9d2-9f80-4454-b603-dd31f71d807e" xlink:to="loc_ifrs-full_AdditionsOtherThanThroughBusinessCombinationsIntangibleAssetsOtherThanGoodwill_3e514687-89c6-4e47-ad78-d4c722adccf0" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_UpfrontPaymentAcquisitionOfIntangibleAsset_ea2ab515-aba3-4b90-963c-728b85483d41" xlink:href="bntx-20241231.xsd#bntx_UpfrontPaymentAcquisitionOfIntangibleAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfIntangibleAssetsLineItems_97e5f9d2-9f80-4454-b603-dd31f71d807e" xlink:to="loc_bntx_UpfrontPaymentAcquisitionOfIntangibleAsset_ea2ab515-aba3-4b90-963c-728b85483d41" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/OtherIntangibleAssetsandGoodwillSummaryofDetailedInformationAboutIntangibleAssetsDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#OtherIntangibleAssetsandGoodwillSummaryofDetailedInformationAboutIntangibleAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/OtherIntangibleAssetsandGoodwillSummaryofDetailedInformationAboutIntangibleAssetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_a7ea00c7-5938-4863-a6dd-d36a75f64553" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillTable_4989f963-3c64-4339-9744-e3f38247bb0d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_a7ea00c7-5938-4863-a6dd-d36a75f64553" xlink:to="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillTable_4989f963-3c64-4339-9744-e3f38247bb0d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis_c4cf09be-2d3c-4659-86f6-b0eb5b918977" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillTable_4989f963-3c64-4339-9744-e3f38247bb0d" xlink:to="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis_c4cf09be-2d3c-4659-86f6-b0eb5b918977" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_c4cf09be-2d3c-4659-86f6-b0eb5b918977_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis_c4cf09be-2d3c-4659-86f6-b0eb5b918977" xlink:to="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_c4cf09be-2d3c-4659-86f6-b0eb5b918977_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_4ce6c8ef-df7e-4cd9-903c-1ea21612222d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillAxis_c4cf09be-2d3c-4659-86f6-b0eb5b918977" xlink:to="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_4ce6c8ef-df7e-4cd9-903c-1ea21612222d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_InProcessRDMember_1a0c2931-7e33-4ea8-9b94-a393b93a3b54" xlink:href="bntx-20241231.xsd#bntx_InProcessRDMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_4ce6c8ef-df7e-4cd9-903c-1ea21612222d" xlink:to="loc_bntx_InProcessRDMember_1a0c2931-7e33-4ea8-9b94-a393b93a3b54" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ConcessionsLicensesAndSimilarRightsMember_2f723194-5a2d-4d7f-b0c9-69ce32919c21" xlink:href="bntx-20241231.xsd#bntx_ConcessionsLicensesAndSimilarRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_4ce6c8ef-df7e-4cd9-903c-1ea21612222d" xlink:to="loc_bntx_ConcessionsLicensesAndSimilarRightsMember_2f723194-5a2d-4d7f-b0c9-69ce32919c21" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_AdvancePaymentsMember_a42fe537-c737-48fc-a4fc-ae3d2ee04fad" xlink:href="bntx-20241231.xsd#bntx_AdvancePaymentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsAndGoodwillDomain_4ce6c8ef-df7e-4cd9-903c-1ea21612222d" xlink:to="loc_bntx_AdvancePaymentsMember_a42fe537-c737-48fc-a4fc-ae3d2ee04fad" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_516a51a0-dbd0-47e3-9a17-2a1c93f4cbb2" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillTable_4989f963-3c64-4339-9744-e3f38247bb0d" xlink:to="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_516a51a0-dbd0-47e3-9a17-2a1c93f4cbb2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountMember_516a51a0-dbd0-47e3-9a17-2a1c93f4cbb2_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_516a51a0-dbd0-47e3-9a17-2a1c93f4cbb2" xlink:to="loc_ifrs-full_CarryingAmountMember_516a51a0-dbd0-47e3-9a17-2a1c93f4cbb2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountMember_952069af-227a-47a9-af18-6f8104d73598" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_516a51a0-dbd0-47e3-9a17-2a1c93f4cbb2" xlink:to="loc_ifrs-full_CarryingAmountMember_952069af-227a-47a9-af18-6f8104d73598" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GrossCarryingAmountMember_3e329770-433f-4cb6-816a-bbf80027d10a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_GrossCarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CarryingAmountMember_952069af-227a-47a9-af18-6f8104d73598" xlink:to="loc_ifrs-full_GrossCarryingAmountMember_3e329770-433f-4cb6-816a-bbf80027d10a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AccumulatedDepreciationAmortisationAndImpairmentMember_06606bdf-cd07-43eb-87ea-6635c92e9309" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AccumulatedDepreciationAmortisationAndImpairmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CarryingAmountMember_952069af-227a-47a9-af18-6f8104d73598" xlink:to="loc_ifrs-full_AccumulatedDepreciationAmortisationAndImpairmentMember_06606bdf-cd07-43eb-87ea-6635c92e9309" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IntangibleAssetsAndGoodwill_5ad20c02-6f61-49c0-bcbd-9e3ee0cd5d14" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IntangibleAssetsAndGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_a7ea00c7-5938-4863-a6dd-d36a75f64553" xlink:to="loc_ifrs-full_IntangibleAssetsAndGoodwill_5ad20c02-6f61-49c0-bcbd-9e3ee0cd5d14" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdditionsOtherThanThroughBusinessCombinationsIntangibleAssetsOtherThanGoodwill_cd6fc254-3adb-4734-ae07-da1f43b2ed34" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AdditionsOtherThanThroughBusinessCombinationsIntangibleAssetsOtherThanGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_a7ea00c7-5938-4863-a6dd-d36a75f64553" xlink:to="loc_ifrs-full_AdditionsOtherThanThroughBusinessCombinationsIntangibleAssetsOtherThanGoodwill_cd6fc254-3adb-4734-ae07-da1f43b2ed34" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_AccumulatedDepreciationAmortisationAndImpairment_9a3871dc-416e-4639-97f0-8d9e8bb1abd5" xlink:href="bntx-20241231.xsd#bntx_AccumulatedDepreciationAmortisationAndImpairment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_a7ea00c7-5938-4863-a6dd-d36a75f64553" xlink:to="loc_bntx_AccumulatedDepreciationAmortisationAndImpairment_9a3871dc-416e-4639-97f0-8d9e8bb1abd5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ImpairmentLossRecognisedInProfitOrLossIntangibleAssetsAndGoodwill_6071fddf-0e2b-4ae3-adf5-a427a64b18aa" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ImpairmentLossRecognisedInProfitOrLossIntangibleAssetsAndGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_a7ea00c7-5938-4863-a6dd-d36a75f64553" xlink:to="loc_ifrs-full_ImpairmentLossRecognisedInProfitOrLossIntangibleAssetsAndGoodwill_6071fddf-0e2b-4ae3-adf5-a427a64b18aa" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisposalsIntangibleAssetsAndGoodwill_5f8b95b4-aeb6-4e13-9bdc-c114b6286afa" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisposalsIntangibleAssetsAndGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_a7ea00c7-5938-4863-a6dd-d36a75f64553" xlink:to="loc_ifrs-full_DisposalsIntangibleAssetsAndGoodwill_5f8b95b4-aeb6-4e13-9bdc-c114b6286afa" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughTransfersAndOtherChangesIntangibleAssetsAndGoodwill_b01dba3d-e76d-468d-8bad-d4d1bfa11c8f" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IncreaseDecreaseThroughTransfersAndOtherChangesIntangibleAssetsAndGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_a7ea00c7-5938-4863-a6dd-d36a75f64553" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughTransfersAndOtherChangesIntangibleAssetsAndGoodwill_b01dba3d-e76d-468d-8bad-d4d1bfa11c8f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesIntangibleAssetsAndGoodwill_d672e3ea-5a06-4c8a-a7e6-1198cd1368f3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesIntangibleAssetsAndGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_a7ea00c7-5938-4863-a6dd-d36a75f64553" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesIntangibleAssetsAndGoodwill_d672e3ea-5a06-4c8a-a7e6-1198cd1368f3" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AcquisitionsThroughBusinessCombinationsIntangibleAssetsAndGoodwill_dbaaf012-fcff-46c6-a7dd-6021dbffcd77" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AcquisitionsThroughBusinessCombinationsIntangibleAssetsAndGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfChangesInIntangibleAssetsAndGoodwillLineItems_a7ea00c7-5938-4863-a6dd-d36a75f64553" xlink:to="loc_ifrs-full_AcquisitionsThroughBusinessCombinationsIntangibleAssetsAndGoodwill_dbaaf012-fcff-46c6-a7dd-6021dbffcd77" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IntangibleAssetsAndGoodwill_939b9737-321e-4567-a704-499f882ad382" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IntangibleAssetsAndGoodwill"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/PropertyPlantandEquipmentSummaryofPropertyPlantandEquipmentDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#PropertyPlantandEquipmentSummaryofPropertyPlantandEquipmentDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/PropertyPlantandEquipmentSummaryofPropertyPlantandEquipmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_4e4b4401-5e9e-4861-8ba2-18cbbce7f3d7" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_c94d924b-49c8-4258-b42c-401db3569d78" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_4e4b4401-5e9e-4861-8ba2-18cbbce7f3d7" xlink:to="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_c94d924b-49c8-4258-b42c-401db3569d78" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_c8adde09-64a2-4d97-b73f-8759a19cb5f3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_c94d924b-49c8-4258-b42c-401db3569d78" xlink:to="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_c8adde09-64a2-4d97-b73f-8759a19cb5f3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_c8adde09-64a2-4d97-b73f-8759a19cb5f3_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_c8adde09-64a2-4d97-b73f-8759a19cb5f3" xlink:to="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_c8adde09-64a2-4d97-b73f-8759a19cb5f3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_b4040d6d-a3c9-46c4-97d1-dbc3bccf83ba" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_c8adde09-64a2-4d97-b73f-8759a19cb5f3" xlink:to="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_b4040d6d-a3c9-46c4-97d1-dbc3bccf83ba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LandAndBuildingsMember_c658f393-9bbd-4935-a9d3-5d6fc2d72595" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LandAndBuildingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_b4040d6d-a3c9-46c4-97d1-dbc3bccf83ba" xlink:to="loc_ifrs-full_LandAndBuildingsMember_c658f393-9bbd-4935-a9d3-5d6fc2d72595" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EquipmentToolsAndInstallationsMember_1f2c41fe-071a-44f8-bbe2-29027bddfc45" xlink:href="bntx-20241231.xsd#bntx_EquipmentToolsAndInstallationsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_b4040d6d-a3c9-46c4-97d1-dbc3bccf83ba" xlink:to="loc_bntx_EquipmentToolsAndInstallationsMember_1f2c41fe-071a-44f8-bbe2-29027bddfc45" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ConstructionInProgressAndAdvancePaymentsMember_a7d051c8-e378-4fe2-b044-c42300a648e0" xlink:href="bntx-20241231.xsd#bntx_ConstructionInProgressAndAdvancePaymentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_b4040d6d-a3c9-46c4-97d1-dbc3bccf83ba" xlink:to="loc_bntx_ConstructionInProgressAndAdvancePaymentsMember_a7d051c8-e378-4fe2-b044-c42300a648e0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_697fe245-6930-4783-9c06-4f8b1bcada81" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_c94d924b-49c8-4258-b42c-401db3569d78" xlink:to="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_697fe245-6930-4783-9c06-4f8b1bcada81" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountMember_697fe245-6930-4783-9c06-4f8b1bcada81_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_697fe245-6930-4783-9c06-4f8b1bcada81" xlink:to="loc_ifrs-full_CarryingAmountMember_697fe245-6930-4783-9c06-4f8b1bcada81_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountMember_6a5732d8-6feb-4c6f-bc49-62d6e55c1057" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_697fe245-6930-4783-9c06-4f8b1bcada81" xlink:to="loc_ifrs-full_CarryingAmountMember_6a5732d8-6feb-4c6f-bc49-62d6e55c1057" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GrossCarryingAmountMember_079faab8-6dc8-49e8-afbb-497099269571" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_GrossCarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CarryingAmountMember_6a5732d8-6feb-4c6f-bc49-62d6e55c1057" xlink:to="loc_ifrs-full_GrossCarryingAmountMember_079faab8-6dc8-49e8-afbb-497099269571" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AccumulatedDepreciationAmortisationAndImpairmentMember_5ca00370-2cfc-45e6-8f50-dacaa4c18a61" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AccumulatedDepreciationAmortisationAndImpairmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CarryingAmountMember_6a5732d8-6feb-4c6f-bc49-62d6e55c1057" xlink:to="loc_ifrs-full_AccumulatedDepreciationAmortisationAndImpairmentMember_5ca00370-2cfc-45e6-8f50-dacaa4c18a61" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PropertyPlantAndEquipment_1c60ac80-3fe1-4875-9e8b-9c08d9b5ed22" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_PropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_4e4b4401-5e9e-4861-8ba2-18cbbce7f3d7" xlink:to="loc_ifrs-full_PropertyPlantAndEquipment_1c60ac80-3fe1-4875-9e8b-9c08d9b5ed22" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdditionsOtherThanThroughBusinessCombinationsPropertyPlantAndEquipment_b9800a24-c2c2-4773-9f6b-f7eb8a711c6b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AdditionsOtherThanThroughBusinessCombinationsPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_4e4b4401-5e9e-4861-8ba2-18cbbce7f3d7" xlink:to="loc_ifrs-full_AdditionsOtherThanThroughBusinessCombinationsPropertyPlantAndEquipment_b9800a24-c2c2-4773-9f6b-f7eb8a711c6b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisposalsPropertyPlantAndEquipment_51ba8056-833b-412a-a7a3-d1914e76e13b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisposalsPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_4e4b4401-5e9e-4861-8ba2-18cbbce7f3d7" xlink:to="loc_ifrs-full_DisposalsPropertyPlantAndEquipment_51ba8056-833b-412a-a7a3-d1914e76e13b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ReclassificationsOfPropertyPlantAndEquipment_cfc509c0-5e79-45f3-818e-2c5fbb6c255f" xlink:href="bntx-20241231.xsd#bntx_ReclassificationsOfPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_4e4b4401-5e9e-4861-8ba2-18cbbce7f3d7" xlink:to="loc_bntx_ReclassificationsOfPropertyPlantAndEquipment_cfc509c0-5e79-45f3-818e-2c5fbb6c255f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesPropertyPlantAndEquipment_01a1f938-705f-4258-b4bb-4101c695a441" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_4e4b4401-5e9e-4861-8ba2-18cbbce7f3d7" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesPropertyPlantAndEquipment_01a1f938-705f-4258-b4bb-4101c695a441" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AcquisitionsThroughBusinessCombinationsPropertyPlantAndEquipment_c00e86dc-590d-4907-867b-e2327f4a1cd8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AcquisitionsThroughBusinessCombinationsPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_4e4b4401-5e9e-4861-8ba2-18cbbce7f3d7" xlink:to="loc_ifrs-full_AcquisitionsThroughBusinessCombinationsPropertyPlantAndEquipment_c00e86dc-590d-4907-867b-e2327f4a1cd8" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DepreciationPropertyPlantAndEquipment_152d6d02-2018-4f5e-b632-62151858e1ec" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DepreciationPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_4e4b4401-5e9e-4861-8ba2-18cbbce7f3d7" xlink:to="loc_ifrs-full_DepreciationPropertyPlantAndEquipment_152d6d02-2018-4f5e-b632-62151858e1ec" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ImpairmentLossRecognisedInProfitOrLossPropertyPlantAndEquipment_9b938bf1-9fe0-4ba7-af50-46624480a03a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ImpairmentLossRecognisedInProfitOrLossPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_4e4b4401-5e9e-4861-8ba2-18cbbce7f3d7" xlink:to="loc_ifrs-full_ImpairmentLossRecognisedInProfitOrLossPropertyPlantAndEquipment_9b938bf1-9fe0-4ba7-af50-46624480a03a" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PropertyPlantAndEquipment_f3cad4b8-a993-4118-ae74-7c1889d28537" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_PropertyPlantAndEquipment"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/PropertyPlantandEquipmentAdditionalInformationDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#PropertyPlantandEquipmentAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/PropertyPlantandEquipmentAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_cf6f3ea7-705c-4abe-8954-2b5408b35c7c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_d427e557-ddc9-4447-85f2-677fac347ff9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_cf6f3ea7-705c-4abe-8954-2b5408b35c7c" xlink:to="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_d427e557-ddc9-4447-85f2-677fac347ff9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_1d078e32-e61d-4b4d-a76c-b6feaec64e1a" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_d427e557-ddc9-4447-85f2-677fac347ff9" xlink:to="loc_srt_StatementGeographicalAxis_1d078e32-e61d-4b4d-a76c-b6feaec64e1a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_1d078e32-e61d-4b4d-a76c-b6feaec64e1a_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_1d078e32-e61d-4b4d-a76c-b6feaec64e1a" xlink:to="loc_srt_SegmentGeographicalDomain_1d078e32-e61d-4b4d-a76c-b6feaec64e1a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_ae2ea9d1-680b-48a0-b9a4-8696b52bde18" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_1d078e32-e61d-4b4d-a76c-b6feaec64e1a" xlink:to="loc_srt_SegmentGeographicalDomain_ae2ea9d1-680b-48a0-b9a4-8696b52bde18" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_39a8f64d-61e5-47c6-9a57-8e3bada09b70" xlink:href="https://xbrl.sec.gov/country/2024/country-2024.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_ae2ea9d1-680b-48a0-b9a4-8696b52bde18" xlink:to="loc_country_US_39a8f64d-61e5-47c6-9a57-8e3bada09b70" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_GB_cc3e56b4-c026-4d67-b726-871fb2c22bf9" xlink:href="https://xbrl.sec.gov/country/2024/country-2024.xsd#country_GB"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_ae2ea9d1-680b-48a0-b9a4-8696b52bde18" xlink:to="loc_country_GB_cc3e56b4-c026-4d67-b726-871fb2c22bf9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_DE_4a951799-b91f-4627-8522-ba7d8f302305" xlink:href="https://xbrl.sec.gov/country/2024/country-2024.xsd#country_DE"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_ae2ea9d1-680b-48a0-b9a4-8696b52bde18" xlink:to="loc_country_DE_4a951799-b91f-4627-8522-ba7d8f302305" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInSubsidiariesAxis_8f8efa93-d943-4adc-9088-217b157185c6" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SignificantInvestmentsInSubsidiariesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentTable_d427e557-ddc9-4447-85f2-677fac347ff9" xlink:to="loc_ifrs-full_SignificantInvestmentsInSubsidiariesAxis_8f8efa93-d943-4adc-9088-217b157185c6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_8f8efa93-d943-4adc-9088-217b157185c6_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SignificantInvestmentsInSubsidiariesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesAxis_8f8efa93-d943-4adc-9088-217b157185c6" xlink:to="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_8f8efa93-d943-4adc-9088-217b157185c6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_7e677448-f9b7-416c-8ae9-c6c071656b3c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SignificantInvestmentsInSubsidiariesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesAxis_8f8efa93-d943-4adc-9088-217b157185c6" xlink:to="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_7e677448-f9b7-416c-8ae9-c6c071656b3c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SubsidiariesMember_96aa81c4-0863-477b-9f62-86e43a7a6d97" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SubsidiariesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_7e677448-f9b7-416c-8ae9-c6c071656b3c" xlink:to="loc_ifrs-full_SubsidiariesMember_96aa81c4-0863-477b-9f62-86e43a7a6d97" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentAssets_d4bc000a-7805-4f59-ade6-9d61175fad70" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NoncurrentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_cf6f3ea7-705c-4abe-8954-2b5408b35c7c" xlink:to="loc_ifrs-full_NoncurrentAssets_d4bc000a-7805-4f59-ade6-9d61175fad70" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofCashandCashEquivalentsDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#FinancialAssetsandFinancialLiabilitiesSummaryofCashandCashEquivalentsDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofCashandCashEquivalentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CashAndCashEquivalents1LineItems_cb9d866f-c4ac-452d-913a-d58ac975eae9" xlink:href="bntx-20241231.xsd#bntx_CashAndCashEquivalents1LineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CashAndCashEquivalentsTable_632a5335-a6f6-497f-bd0e-b41fb55771fd" xlink:href="bntx-20241231.xsd#bntx_CashAndCashEquivalentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_bntx_CashAndCashEquivalents1LineItems_cb9d866f-c4ac-452d-913a-d58ac975eae9" xlink:to="loc_bntx_CashAndCashEquivalentsTable_632a5335-a6f6-497f-bd0e-b41fb55771fd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsAxis_8b9dbf95-06d4-4a48-912f-946251d6a87a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_CashAndCashEquivalentsTable_632a5335-a6f6-497f-bd0e-b41fb55771fd" xlink:to="loc_ifrs-full_ClassesOfAssetsAxis_8b9dbf95-06d4-4a48-912f-946251d6a87a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_8b9dbf95-06d4-4a48-912f-946251d6a87a_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_8b9dbf95-06d4-4a48-912f-946251d6a87a" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_8b9dbf95-06d4-4a48-912f-946251d6a87a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_d8f5c8d4-ec32-41f3-b6b2-f2e9147879ee" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_8b9dbf95-06d4-4a48-912f-946251d6a87a" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_d8f5c8d4-ec32-41f3-b6b2-f2e9147879ee" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BankDepositsMember_d85af072-5bca-4023-8757-aae892e0d83c" xlink:href="bntx-20241231.xsd#bntx_BankDepositsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_d8f5c8d4-ec32-41f3-b6b2-f2e9147879ee" xlink:to="loc_bntx_BankDepositsMember_d85af072-5bca-4023-8757-aae892e0d83c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_MoneyMarketFundMember_0b487401-4823-45e2-8b4e-6def49dc0a91" xlink:href="bntx-20241231.xsd#bntx_MoneyMarketFundMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_d8f5c8d4-ec32-41f3-b6b2-f2e9147879ee" xlink:to="loc_bntx_MoneyMarketFundMember_0b487401-4823-45e2-8b4e-6def49dc0a91" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ReverseRepoMember_19664501-2636-4ef5-baf5-68bf4747f54d" xlink:href="bntx-20241231.xsd#bntx_ReverseRepoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_d8f5c8d4-ec32-41f3-b6b2-f2e9147879ee" xlink:to="loc_bntx_ReverseRepoMember_19664501-2636-4ef5-baf5-68bf4747f54d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Cash_50b94db5-3622-4079-b35f-a4efe226905e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_Cash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_CashAndCashEquivalents1LineItems_cb9d866f-c4ac-452d-913a-d58ac975eae9" xlink:to="loc_ifrs-full_Cash_50b94db5-3622-4079-b35f-a4efe226905e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CashEquivalents_52bd1fd0-6e94-402f-af0e-acf6448097cb" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CashEquivalents"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_CashAndCashEquivalents1LineItems_cb9d866f-c4ac-452d-913a-d58ac975eae9" xlink:to="loc_ifrs-full_CashEquivalents_52bd1fd0-6e94-402f-af0e-acf6448097cb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CashAndCashEquivalents_6625e66b-e117-47e5-a676-fa07a2d618b9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CashAndCashEquivalents"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_CashAndCashEquivalents1LineItems_cb9d866f-c4ac-452d-913a-d58ac975eae9" xlink:to="loc_ifrs-full_CashAndCashEquivalents_6625e66b-e117-47e5-a676-fa07a2d618b9" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofFinancialAssetsandLiabilitiesatAmortizedCostandatFairValueDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#FinancialAssetsandFinancialLiabilitiesSummaryofFinancialAssetsandLiabilitiesatAmortizedCostandatFairValueDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofFinancialAssetsandLiabilitiesatAmortizedCostandatFairValueDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DisclosureOfFinancialAssetsAndLiabilitiesLineItems_421d3610-caae-4cd8-966e-1d5500949f3f" xlink:href="bntx-20241231.xsd#bntx_DisclosureOfFinancialAssetsAndLiabilitiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DisclosureOfFinancialAssetsAndLiabilitiesTable_3868c155-1f3c-4d10-892e-7f424a5ed8a0" xlink:href="bntx-20241231.xsd#bntx_DisclosureOfFinancialAssetsAndLiabilitiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_bntx_DisclosureOfFinancialAssetsAndLiabilitiesLineItems_421d3610-caae-4cd8-966e-1d5500949f3f" xlink:to="loc_bntx_DisclosureOfFinancialAssetsAndLiabilitiesTable_3868c155-1f3c-4d10-892e-7f424a5ed8a0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsAxis_3bef22ed-7b67-46ea-9ebc-895012f577ca" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfFinancialAssetsAndLiabilitiesTable_3868c155-1f3c-4d10-892e-7f424a5ed8a0" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsAxis_3bef22ed-7b67-46ea-9ebc-895012f577ca" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_3bef22ed-7b67-46ea-9ebc-895012f577ca_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_3bef22ed-7b67-46ea-9ebc-895012f577ca" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_3bef22ed-7b67-46ea-9ebc-895012f577ca_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_dfc9cb3b-7d8e-4ed5-86ec-f2f3771c45d6" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_3bef22ed-7b67-46ea-9ebc-895012f577ca" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_dfc9cb3b-7d8e-4ed5-86ec-f2f3771c45d6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ForwardContractMember_53aaa371-98b7-4989-a69d-e95f94062631" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ForwardContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_dfc9cb3b-7d8e-4ed5-86ec-f2f3771c45d6" xlink:to="loc_ifrs-full_ForwardContractMember_53aaa371-98b7-4989-a69d-e95f94062631" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_SecurityInvestmentsDisclosedAsCashAndCashEquivalentsMember_db6a0298-b943-4cc0-8dca-41c5b4fe7d23" xlink:href="bntx-20241231.xsd#bntx_SecurityInvestmentsDisclosedAsCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_dfc9cb3b-7d8e-4ed5-86ec-f2f3771c45d6" xlink:to="loc_bntx_SecurityInvestmentsDisclosedAsCashAndCashEquivalentsMember_db6a0298-b943-4cc0-8dca-41c5b4fe7d23" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OtherFinancialAssetsMember_f0ecb2e2-eb72-49c2-9970-ff170be586b6" xlink:href="bntx-20241231.xsd#bntx_OtherFinancialAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_dfc9cb3b-7d8e-4ed5-86ec-f2f3771c45d6" xlink:to="loc_bntx_OtherFinancialAssetsMember_f0ecb2e2-eb72-49c2-9970-ff170be586b6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NonListedEquityInvestmentsMember_55736284-9482-4adf-b306-b87dc5043a72" xlink:href="bntx-20241231.xsd#bntx_NonListedEquityInvestmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_dfc9cb3b-7d8e-4ed5-86ec-f2f3771c45d6" xlink:to="loc_bntx_NonListedEquityInvestmentsMember_55736284-9482-4adf-b306-b87dc5043a72" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ListedEquityInvestmentsMember_093ac49c-959b-4eb5-b661-ef4b8f0e8aba" xlink:href="bntx-20241231.xsd#bntx_ListedEquityInvestmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_dfc9cb3b-7d8e-4ed5-86ec-f2f3771c45d6" xlink:to="loc_bntx_ListedEquityInvestmentsMember_093ac49c-959b-4eb5-b661-ef4b8f0e8aba" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_SecurityInvestmentsDisclosedAsOtherFinancialAssetsMember_0bfa1aeb-439f-40aa-876f-7ca74b91f4fb" xlink:href="bntx-20241231.xsd#bntx_SecurityInvestmentsDisclosedAsOtherFinancialAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_dfc9cb3b-7d8e-4ed5-86ec-f2f3771c45d6" xlink:to="loc_bntx_SecurityInvestmentsDisclosedAsOtherFinancialAssetsMember_0bfa1aeb-439f-40aa-876f-7ca74b91f4fb" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CashAtBanksAndOnHandMember_755b3976-3887-428e-ac24-2be144b09c66" xlink:href="bntx-20241231.xsd#bntx_CashAtBanksAndOnHandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_dfc9cb3b-7d8e-4ed5-86ec-f2f3771c45d6" xlink:to="loc_bntx_CashAtBanksAndOnHandMember_755b3976-3887-428e-ac24-2be144b09c66" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_TradeAndOtherReceivablesMember_efa5b24d-f40c-424b-b0a2-4bd9a90599d7" xlink:href="bntx-20241231.xsd#bntx_TradeAndOtherReceivablesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_dfc9cb3b-7d8e-4ed5-86ec-f2f3771c45d6" xlink:to="loc_bntx_TradeAndOtherReceivablesMember_efa5b24d-f40c-424b-b0a2-4bd9a90599d7" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ReimbursementAssetMember_f97cd700-4401-438a-96fd-da446300173b" xlink:href="bntx-20241231.xsd#bntx_ReimbursementAssetMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_dfc9cb3b-7d8e-4ed5-86ec-f2f3771c45d6" xlink:to="loc_bntx_ReimbursementAssetMember_f97cd700-4401-438a-96fd-da446300173b" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_49b8cc25-37d5-4c3f-bfab-cc4e6987c43a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfFinancialAssetsAndLiabilitiesTable_3868c155-1f3c-4d10-892e-7f424a5ed8a0" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_49b8cc25-37d5-4c3f-bfab-cc4e6987c43a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_49b8cc25-37d5-4c3f-bfab-cc4e6987c43a_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_49b8cc25-37d5-4c3f-bfab-cc4e6987c43a" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_49b8cc25-37d5-4c3f-bfab-cc4e6987c43a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_829ac550-9712-4448-bd08-9e67f938b4e9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_49b8cc25-37d5-4c3f-bfab-cc4e6987c43a" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_829ac550-9712-4448-bd08-9e67f938b4e9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_FairValueThroughProfitOrLossMember_6df3726a-b0cc-45d9-b65b-f8a054d94768" xlink:href="bntx-20241231.xsd#bntx_FairValueThroughProfitOrLossMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_829ac550-9712-4448-bd08-9e67f938b4e9" xlink:to="loc_bntx_FairValueThroughProfitOrLossMember_6df3726a-b0cc-45d9-b65b-f8a054d94768" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_FairValueThroughOCIMember_3d585a52-5e72-4791-9088-ff72f047c953" xlink:href="bntx-20241231.xsd#bntx_FairValueThroughOCIMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_829ac550-9712-4448-bd08-9e67f938b4e9" xlink:to="loc_bntx_FairValueThroughOCIMember_3d585a52-5e72-4791-9088-ff72f047c953" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_AmortizedCostMember_ff408877-7922-434c-b6ce-b91e8e420f78" xlink:href="bntx-20241231.xsd#bntx_AmortizedCostMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_829ac550-9712-4448-bd08-9e67f938b4e9" xlink:to="loc_bntx_AmortizedCostMember_ff408877-7922-434c-b6ce-b91e8e420f78" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LevelsOfFairValueHierarchyAxis_e0bfacb3-76d5-41a2-8f43-77daa5426fb6" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LevelsOfFairValueHierarchyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfFinancialAssetsAndLiabilitiesTable_3868c155-1f3c-4d10-892e-7f424a5ed8a0" xlink:to="loc_ifrs-full_LevelsOfFairValueHierarchyAxis_e0bfacb3-76d5-41a2-8f43-77daa5426fb6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_e0bfacb3-76d5-41a2-8f43-77daa5426fb6_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LevelsOfFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_LevelsOfFairValueHierarchyAxis_e0bfacb3-76d5-41a2-8f43-77daa5426fb6" xlink:to="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_e0bfacb3-76d5-41a2-8f43-77daa5426fb6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_28cdcd02-1413-4d39-8bb1-fd1ddcccd765" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LevelsOfFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_LevelsOfFairValueHierarchyAxis_e0bfacb3-76d5-41a2-8f43-77daa5426fb6" xlink:to="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_28cdcd02-1413-4d39-8bb1-fd1ddcccd765" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Level1OfFairValueHierarchyMember_94126177-af04-440e-b310-d48084be0209" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_Level1OfFairValueHierarchyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_28cdcd02-1413-4d39-8bb1-fd1ddcccd765" xlink:to="loc_ifrs-full_Level1OfFairValueHierarchyMember_94126177-af04-440e-b310-d48084be0209" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Level2OfFairValueHierarchyMember_c2d66edb-7cec-4bee-bd9a-d0a094ff6308" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_Level2OfFairValueHierarchyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_28cdcd02-1413-4d39-8bb1-fd1ddcccd765" xlink:to="loc_ifrs-full_Level2OfFairValueHierarchyMember_c2d66edb-7cec-4bee-bd9a-d0a094ff6308" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Level3OfFairValueHierarchyMember_a38e3cec-d69b-4bda-a100-4110c21b1d1d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_Level3OfFairValueHierarchyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_28cdcd02-1413-4d39-8bb1-fd1ddcccd765" xlink:to="loc_ifrs-full_Level3OfFairValueHierarchyMember_a38e3cec-d69b-4bda-a100-4110c21b1d1d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialLiabilitiesAxis_5f92545b-94f2-4717-ab27-3ffb3c9ba9c8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialLiabilitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfFinancialAssetsAndLiabilitiesTable_3868c155-1f3c-4d10-892e-7f424a5ed8a0" xlink:to="loc_ifrs-full_ClassesOfFinancialLiabilitiesAxis_5f92545b-94f2-4717-ab27-3ffb3c9ba9c8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_5f92545b-94f2-4717-ab27-3ffb3c9ba9c8_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialLiabilitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialLiabilitiesAxis_5f92545b-94f2-4717-ab27-3ffb3c9ba9c8" xlink:to="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_5f92545b-94f2-4717-ab27-3ffb3c9ba9c8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_d81c06bd-b73d-4258-bba9-a588ddb50a8c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialLiabilitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialLiabilitiesAxis_5f92545b-94f2-4717-ab27-3ffb3c9ba9c8" xlink:to="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_d81c06bd-b73d-4258-bba9-a588ddb50a8c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ForwardContractMember_46e6a5dd-7ec7-46b4-9389-fe9bf87ace7b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ForwardContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_d81c06bd-b73d-4258-bba9-a588ddb50a8c" xlink:to="loc_ifrs-full_ForwardContractMember_46e6a5dd-7ec7-46b4-9389-fe9bf87ace7b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ContingentConsiderationMember_a5678445-08f4-43dc-9fa2-800a1319412b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ContingentConsiderationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_d81c06bd-b73d-4258-bba9-a588ddb50a8c" xlink:to="loc_ifrs-full_ContingentConsiderationMember_a5678445-08f4-43dc-9fa2-800a1319412b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_LoansAndBorrowingsMember_d2b44205-81cb-49ae-991b-f546d5015d41" xlink:href="bntx-20241231.xsd#bntx_LoansAndBorrowingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_d81c06bd-b73d-4258-bba9-a588ddb50a8c" xlink:to="loc_bntx_LoansAndBorrowingsMember_d2b44205-81cb-49ae-991b-f546d5015d41" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_TradePayablesAndOtherPayablesMember_10affcb1-339e-480d-b266-97c5a6e295e3" xlink:href="bntx-20241231.xsd#bntx_TradePayablesAndOtherPayablesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_d81c06bd-b73d-4258-bba9-a588ddb50a8c" xlink:to="loc_bntx_TradePayablesAndOtherPayablesMember_10affcb1-339e-480d-b266-97c5a6e295e3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OtherFinancialLiabilitiesMember_dd7e726a-258f-4f4a-9b4b-170aaf11f926" xlink:href="bntx-20241231.xsd#bntx_OtherFinancialLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_d81c06bd-b73d-4258-bba9-a588ddb50a8c" xlink:to="loc_bntx_OtherFinancialLiabilitiesMember_dd7e726a-258f-4f4a-9b4b-170aaf11f926" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LeaseLiabilitiesMember_7438526e-0f52-4088-8a0c-4ec0fe361855" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LeaseLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_d81c06bd-b73d-4258-bba9-a588ddb50a8c" xlink:to="loc_ifrs-full_LeaseLiabilitiesMember_7438526e-0f52-4088-8a0c-4ec0fe361855" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfFinancialLiabilitiesAxis_d7b7d8d9-cc6c-4165-b95f-828de5201f4c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfFinancialLiabilitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfFinancialAssetsAndLiabilitiesTable_3868c155-1f3c-4d10-892e-7f424a5ed8a0" xlink:to="loc_ifrs-full_CategoriesOfFinancialLiabilitiesAxis_d7b7d8d9-cc6c-4165-b95f-828de5201f4c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfFinancialLiabilitiesDomain_d7b7d8d9-cc6c-4165-b95f-828de5201f4c_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfFinancialLiabilitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CategoriesOfFinancialLiabilitiesAxis_d7b7d8d9-cc6c-4165-b95f-828de5201f4c" xlink:to="loc_ifrs-full_CategoriesOfFinancialLiabilitiesDomain_d7b7d8d9-cc6c-4165-b95f-828de5201f4c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfFinancialLiabilitiesDomain_f0585afa-8b4e-44bc-9146-a8666d9795cc" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfFinancialLiabilitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CategoriesOfFinancialLiabilitiesAxis_d7b7d8d9-cc6c-4165-b95f-828de5201f4c" xlink:to="loc_ifrs-full_CategoriesOfFinancialLiabilitiesDomain_f0585afa-8b4e-44bc-9146-a8666d9795cc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_FinancialLiabilitiesAtFairValueThroughOtherComprehensiveIncomeCategoryMember_71bec797-f409-43d2-b9fc-90ed93c960ed" xlink:href="bntx-20241231.xsd#bntx_FinancialLiabilitiesAtFairValueThroughOtherComprehensiveIncomeCategoryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfFinancialLiabilitiesDomain_f0585afa-8b4e-44bc-9146-a8666d9795cc" xlink:to="loc_bntx_FinancialLiabilitiesAtFairValueThroughOtherComprehensiveIncomeCategoryMember_71bec797-f409-43d2-b9fc-90ed93c960ed" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinancialLiabilitiesAtAmortisedCostCategoryMember_35de7efa-823b-405a-ba4d-a570734bbf46" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_FinancialLiabilitiesAtAmortisedCostCategoryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfFinancialLiabilitiesDomain_f0585afa-8b4e-44bc-9146-a8666d9795cc" xlink:to="loc_ifrs-full_FinancialLiabilitiesAtAmortisedCostCategoryMember_35de7efa-823b-405a-ba4d-a570734bbf46" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_FinancialLiabilitiesSubsequentlyNotMeasuredAccordingToIFRS9Member_7c8f96e0-a291-41c1-8957-1354106ef0e6" xlink:href="bntx-20241231.xsd#bntx_FinancialLiabilitiesSubsequentlyNotMeasuredAccordingToIFRS9Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfFinancialLiabilitiesDomain_f0585afa-8b4e-44bc-9146-a8666d9795cc" xlink:to="loc_bntx_FinancialLiabilitiesSubsequentlyNotMeasuredAccordingToIFRS9Member_7c8f96e0-a291-41c1-8957-1354106ef0e6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfFinancialAssetsAbstract_e8b35630-b16b-4d11-b15f-fa5d8366de71" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfFinancialAssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfFinancialAssetsAndLiabilitiesLineItems_421d3610-caae-4cd8-966e-1d5500949f3f" xlink:to="loc_ifrs-full_CategoriesOfFinancialAssetsAbstract_e8b35630-b16b-4d11-b15f-fa5d8366de71" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentFinancialAssets_8df734ab-6dd9-4424-aa20-fd7e144c42c2" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CurrentFinancialAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfFinancialAssetsAbstract_e8b35630-b16b-4d11-b15f-fa5d8366de71" xlink:to="loc_ifrs-full_CurrentFinancialAssets_8df734ab-6dd9-4424-aa20-fd7e144c42c2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentFinancialAssets_a1d5e574-49d7-4521-a7da-c405701132b1" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NoncurrentFinancialAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfFinancialAssetsAbstract_e8b35630-b16b-4d11-b15f-fa5d8366de71" xlink:to="loc_ifrs-full_NoncurrentFinancialAssets_a1d5e574-49d7-4521-a7da-c405701132b1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinancialAssets_6c5b5741-e077-46e7-a883-3a68161f1a22" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_FinancialAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfFinancialAssetsAbstract_e8b35630-b16b-4d11-b15f-fa5d8366de71" xlink:to="loc_ifrs-full_FinancialAssets_6c5b5741-e077-46e7-a883-3a68161f1a22" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfFinancialLiabilitiesAbstract_fe02618b-3d83-4c99-b0e7-c9b54c26a49b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfFinancialLiabilitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfFinancialAssetsAndLiabilitiesLineItems_421d3610-caae-4cd8-966e-1d5500949f3f" xlink:to="loc_ifrs-full_CategoriesOfFinancialLiabilitiesAbstract_fe02618b-3d83-4c99-b0e7-c9b54c26a49b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentFinancialLiabilities_1e126890-d171-4317-b929-f8dffd74fa3f" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CurrentFinancialLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfFinancialLiabilitiesAbstract_fe02618b-3d83-4c99-b0e7-c9b54c26a49b" xlink:to="loc_ifrs-full_CurrentFinancialLiabilities_1e126890-d171-4317-b929-f8dffd74fa3f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentFinancialLiabilities_cb99bce4-f7ea-4c0a-b79f-c7e9a0c26a02" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NoncurrentFinancialLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfFinancialLiabilitiesAbstract_fe02618b-3d83-4c99-b0e7-c9b54c26a49b" xlink:to="loc_ifrs-full_NoncurrentFinancialLiabilities_cb99bce4-f7ea-4c0a-b79f-c7e9a0c26a02" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinancialLiabilities_3505c584-380f-47c2-a7cc-2ef3eb5d4505" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_FinancialLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfFinancialLiabilitiesAbstract_fe02618b-3d83-4c99-b0e7-c9b54c26a49b" xlink:to="loc_ifrs-full_FinancialLiabilities_3505c584-380f-47c2-a7cc-2ef3eb5d4505" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofEquitySecuritiesMeasuredatFairValueThroughOtherComprehensiveIncomeDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#FinancialAssetsandFinancialLiabilitiesSummaryofEquitySecuritiesMeasuredatFairValueThroughOtherComprehensiveIncomeDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofEquitySecuritiesMeasuredatFairValueThroughOtherComprehensiveIncomeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfFinancialAssetsLineItems_0b010ef0-8ef8-4e92-86bf-1f625c8da324" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfFinancialAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfFinancialAssetsTable_ef51a3fc-22b3-4d5c-82b7-823d863f97ea" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfFinancialAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfFinancialAssetsLineItems_0b010ef0-8ef8-4e92-86bf-1f625c8da324" xlink:to="loc_ifrs-full_DisclosureOfFinancialAssetsTable_ef51a3fc-22b3-4d5c-82b7-823d863f97ea" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfFinancialAssetsAxis_48282feb-fd3d-403a-94a9-c939dd33baa7" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfFinancialAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfFinancialAssetsTable_ef51a3fc-22b3-4d5c-82b7-823d863f97ea" xlink:to="loc_ifrs-full_CategoriesOfFinancialAssetsAxis_48282feb-fd3d-403a-94a9-c939dd33baa7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfFinancialAssetsDomain_48282feb-fd3d-403a-94a9-c939dd33baa7_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CategoriesOfFinancialAssetsAxis_48282feb-fd3d-403a-94a9-c939dd33baa7" xlink:to="loc_ifrs-full_CategoriesOfFinancialAssetsDomain_48282feb-fd3d-403a-94a9-c939dd33baa7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfFinancialAssetsDomain_f44b7155-4279-420f-b4e3-f3433b64b5e2" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CategoriesOfFinancialAssetsAxis_48282feb-fd3d-403a-94a9-c939dd33baa7" xlink:to="loc_ifrs-full_CategoriesOfFinancialAssetsDomain_f44b7155-4279-420f-b4e3-f3433b64b5e2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinancialAssetsAtFairValueThroughOtherComprehensiveIncomeCategoryMember_819c99d6-f55d-4d5c-aa9c-ed00486f3e40" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_FinancialAssetsAtFairValueThroughOtherComprehensiveIncomeCategoryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfFinancialAssetsDomain_f44b7155-4279-420f-b4e3-f3433b64b5e2" xlink:to="loc_ifrs-full_FinancialAssetsAtFairValueThroughOtherComprehensiveIncomeCategoryMember_819c99d6-f55d-4d5c-aa9c-ed00486f3e40" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherComprehensiveIncomeNetOfTaxGainsLossesFromInvestmentsInEquityInstruments_33397724-4f80-4374-81a1-cf43753209ed" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_OtherComprehensiveIncomeNetOfTaxGainsLossesFromInvestmentsInEquityInstruments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialAssetsLineItems_0b010ef0-8ef8-4e92-86bf-1f625c8da324" xlink:to="loc_ifrs-full_OtherComprehensiveIncomeNetOfTaxGainsLossesFromInvestmentsInEquityInstruments_33397724-4f80-4374-81a1-cf43753209ed" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofSignificantUnobservableInputsLevel3UsedforRecurringFairValueMeasurementsandtheEffectoftheMeasurementsDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#FinancialAssetsandFinancialLiabilitiesSummaryofSignificantUnobservableInputsLevel3UsedforRecurringFairValueMeasurementsandtheEffectoftheMeasurementsDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofSignificantUnobservableInputsLevel3UsedforRecurringFairValueMeasurementsandtheEffectoftheMeasurementsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_11825e72-1990-4010-b591-3ccbca26e1f0" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfFinancialLiabilitiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_e5ae0897-c983-4ec4-a87e-fd20c542fa1d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfFinancialLiabilitiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_11825e72-1990-4010-b591-3ccbca26e1f0" xlink:to="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_e5ae0897-c983-4ec4-a87e-fd20c542fa1d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LevelsOfFairValueHierarchyAxis_e37f0231-a7d5-40b5-9918-4e3b856e0e6c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LevelsOfFairValueHierarchyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_e5ae0897-c983-4ec4-a87e-fd20c542fa1d" xlink:to="loc_ifrs-full_LevelsOfFairValueHierarchyAxis_e37f0231-a7d5-40b5-9918-4e3b856e0e6c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_e37f0231-a7d5-40b5-9918-4e3b856e0e6c_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LevelsOfFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_LevelsOfFairValueHierarchyAxis_e37f0231-a7d5-40b5-9918-4e3b856e0e6c" xlink:to="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_e37f0231-a7d5-40b5-9918-4e3b856e0e6c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_2081fa0f-6b3e-4d9c-a2d1-253081172abe" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LevelsOfFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_LevelsOfFairValueHierarchyAxis_e37f0231-a7d5-40b5-9918-4e3b856e0e6c" xlink:to="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_2081fa0f-6b3e-4d9c-a2d1-253081172abe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Level3OfFairValueHierarchyMember_610b3de1-517b-48a1-8d4b-172ba6fdc019" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_Level3OfFairValueHierarchyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_2081fa0f-6b3e-4d9c-a2d1-253081172abe" xlink:to="loc_ifrs-full_Level3OfFairValueHierarchyMember_610b3de1-517b-48a1-8d4b-172ba6fdc019" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfLiabilitiesAxis_ccbac463-0d8b-46d9-aa1b-521d38749ea7" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfLiabilitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_e5ae0897-c983-4ec4-a87e-fd20c542fa1d" xlink:to="loc_ifrs-full_ClassesOfLiabilitiesAxis_ccbac463-0d8b-46d9-aa1b-521d38749ea7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfLiabilitiesDomain_ccbac463-0d8b-46d9-aa1b-521d38749ea7_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfLiabilitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfLiabilitiesAxis_ccbac463-0d8b-46d9-aa1b-521d38749ea7" xlink:to="loc_ifrs-full_ClassesOfLiabilitiesDomain_ccbac463-0d8b-46d9-aa1b-521d38749ea7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfLiabilitiesDomain_2878eb7e-0d9b-4417-a0c9-0dd3018976dd" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfLiabilitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfLiabilitiesAxis_ccbac463-0d8b-46d9-aa1b-521d38749ea7" xlink:to="loc_ifrs-full_ClassesOfLiabilitiesDomain_2878eb7e-0d9b-4417-a0c9-0dd3018976dd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ContingentConsiderationMember_ab73a37d-6bc8-4be7-bb22-f7d7247cb65c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ContingentConsiderationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfLiabilitiesDomain_2878eb7e-0d9b-4417-a0c9-0dd3018976dd" xlink:to="loc_ifrs-full_ContingentConsiderationMember_ab73a37d-6bc8-4be7-bb22-f7d7247cb65c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsAxis_b5d073c7-2599-4f13-a75c-cd2342993df0" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_e5ae0897-c983-4ec4-a87e-fd20c542fa1d" xlink:to="loc_ifrs-full_ClassesOfAssetsAxis_b5d073c7-2599-4f13-a75c-cd2342993df0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_b5d073c7-2599-4f13-a75c-cd2342993df0_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_b5d073c7-2599-4f13-a75c-cd2342993df0" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_b5d073c7-2599-4f13-a75c-cd2342993df0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_6d14bded-96e3-4a1b-8b12-b195a43e6da3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_b5d073c7-2599-4f13-a75c-cd2342993df0" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_6d14bded-96e3-4a1b-8b12-b195a43e6da3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OtherFinancialAssetsMember_69958cc5-9330-4250-93f2-7f47e022e6cb" xlink:href="bntx-20241231.xsd#bntx_OtherFinancialAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_6d14bded-96e3-4a1b-8b12-b195a43e6da3" xlink:to="loc_bntx_OtherFinancialAssetsMember_69958cc5-9330-4250-93f2-7f47e022e6cb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_LiabilitiesRollForward_1c54bb1e-51fa-48d6-8b2a-3e98f82ebefd" xlink:href="bntx-20241231.xsd#bntx_LiabilitiesRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_11825e72-1990-4010-b591-3ccbca26e1f0" xlink:to="loc_bntx_LiabilitiesRollForward_1c54bb1e-51fa-48d6-8b2a-3e98f82ebefd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinancialLiabilities_de6a23ae-7510-4d7c-a7da-3f5e075f1cb9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_FinancialLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_LiabilitiesRollForward_1c54bb1e-51fa-48d6-8b2a-3e98f82ebefd" xlink:to="loc_ifrs-full_FinancialLiabilities_de6a23ae-7510-4d7c-a7da-3f5e075f1cb9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PurchasesFairValueMeasurementLiabilities_970700db-f556-450f-9904-4274fcb1a7aa" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_PurchasesFairValueMeasurementLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_LiabilitiesRollForward_1c54bb1e-51fa-48d6-8b2a-3e98f82ebefd" xlink:to="loc_ifrs-full_PurchasesFairValueMeasurementLiabilities_970700db-f556-450f-9904-4274fcb1a7aa" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GainsLossesRecognisedInProfitOrLossFairValueMeasurementLiabilities_9097aeec-a02d-4414-8e2a-81bcb92f83bb" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_GainsLossesRecognisedInProfitOrLossFairValueMeasurementLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_LiabilitiesRollForward_1c54bb1e-51fa-48d6-8b2a-3e98f82ebefd" xlink:to="loc_ifrs-full_GainsLossesRecognisedInProfitOrLossFairValueMeasurementLiabilities_9097aeec-a02d-4414-8e2a-81bcb92f83bb" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SalesFairValueMeasurementLiabilities_3c3e2448-5180-483c-8a37-cd2093c92db3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SalesFairValueMeasurementLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_LiabilitiesRollForward_1c54bb1e-51fa-48d6-8b2a-3e98f82ebefd" xlink:to="loc_ifrs-full_SalesFairValueMeasurementLiabilities_3c3e2448-5180-483c-8a37-cd2093c92db3" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinancialLiabilities_64dd47eb-edd0-4bb2-b033-225ffc108044" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_FinancialLiabilities"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_AssetsRollForward_833acdc5-7363-44d2-8eae-14678d9d0e9b" xlink:href="bntx-20241231.xsd#bntx_AssetsRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_11825e72-1990-4010-b591-3ccbca26e1f0" xlink:to="loc_bntx_AssetsRollForward_833acdc5-7363-44d2-8eae-14678d9d0e9b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinancialAssets_9ceca328-d189-47a7-96d7-cd02d7be8b7e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_FinancialAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_AssetsRollForward_833acdc5-7363-44d2-8eae-14678d9d0e9b" xlink:to="loc_ifrs-full_FinancialAssets_9ceca328-d189-47a7-96d7-cd02d7be8b7e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PurchasesFairValueMeasurementAssets_89369952-756f-4ffe-ae26-6b455c37501b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_PurchasesFairValueMeasurementAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_AssetsRollForward_833acdc5-7363-44d2-8eae-14678d9d0e9b" xlink:to="loc_ifrs-full_PurchasesFairValueMeasurementAssets_89369952-756f-4ffe-ae26-6b455c37501b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GainsLossesRecognisedInProfitOrLossFairValueMeasurementAssets_799f29d6-a314-458a-bbe5-8ceac73b2c09" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_GainsLossesRecognisedInProfitOrLossFairValueMeasurementAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_AssetsRollForward_833acdc5-7363-44d2-8eae-14678d9d0e9b" xlink:to="loc_ifrs-full_GainsLossesRecognisedInProfitOrLossFairValueMeasurementAssets_799f29d6-a314-458a-bbe5-8ceac73b2c09" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SalesFairValueMeasurementAssets_6eadd288-7774-4287-97fc-776c6c1f7e32" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SalesFairValueMeasurementAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_AssetsRollForward_833acdc5-7363-44d2-8eae-14678d9d0e9b" xlink:to="loc_ifrs-full_SalesFairValueMeasurementAssets_6eadd288-7774-4287-97fc-776c6c1f7e32" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinancialAssets_cfd0d9e3-da76-4b09-9c02-f47dc1d66b36" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_FinancialAssets"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesNarrativeDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#FinancialAssetsandFinancialLiabilitiesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfFinancialAssetsLineItems_50be2b30-1aa2-4e5c-80c9-c85a8efac08e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfFinancialAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfFinancialAssetsTable_2e778727-1f98-473e-960e-87a7a808fa05" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfFinancialAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfFinancialAssetsLineItems_50be2b30-1aa2-4e5c-80c9-c85a8efac08e" xlink:to="loc_ifrs-full_DisclosureOfFinancialAssetsTable_2e778727-1f98-473e-960e-87a7a808fa05" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_f8f88f14-b08c-4e27-b7f7-bdd5d6c840e0" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfFinancialAssetsTable_2e778727-1f98-473e-960e-87a7a808fa05" xlink:to="loc_srt_CounterpartyNameAxis_f8f88f14-b08c-4e27-b7f7-bdd5d6c840e0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_f8f88f14-b08c-4e27-b7f7-bdd5d6c840e0_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_f8f88f14-b08c-4e27-b7f7-bdd5d6c840e0" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_f8f88f14-b08c-4e27-b7f7-bdd5d6c840e0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_b8dbd4d1-53ee-434d-9d2f-5dfea3f3c6af" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_f8f88f14-b08c-4e27-b7f7-bdd5d6c840e0" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_b8dbd4d1-53ee-434d-9d2f-5dfea3f3c6af" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NationalInstitutesOfHealthMember_6531ffd8-7d59-4c1a-aee8-a0a376b83675" xlink:href="bntx-20241231.xsd#bntx_NationalInstitutesOfHealthMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_b8dbd4d1-53ee-434d-9d2f-5dfea3f3c6af" xlink:to="loc_bntx_NationalInstitutesOfHealthMember_6531ffd8-7d59-4c1a-aee8-a0a376b83675" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_UPennMember_a7e1bd6a-287c-4cf6-812d-50382d498514" xlink:href="bntx-20241231.xsd#bntx_UPennMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_b8dbd4d1-53ee-434d-9d2f-5dfea3f3c6af" xlink:to="loc_bntx_UPennMember_a7e1bd6a-287c-4cf6-812d-50382d498514" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GainsOnLitigationSettlements_31a2c2c2-593a-4ff6-8264-4ee323b77848" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_GainsOnLitigationSettlements"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialAssetsLineItems_50be2b30-1aa2-4e5c-80c9-c85a8efac08e" xlink:to="loc_ifrs-full_GainsOnLitigationSettlements_31a2c2c2-593a-4ff6-8264-4ee323b77848" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LossesOnLitigationSettlements_e1ef323d-6a84-430a-a7be-9841b90bda10" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LossesOnLitigationSettlements"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialAssetsLineItems_50be2b30-1aa2-4e5c-80c9-c85a8efac08e" xlink:to="loc_ifrs-full_LossesOnLitigationSettlements_e1ef323d-6a84-430a-a7be-9841b90bda10" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_LitigationSettlmentContributionToResearchAndDevelopment_199f2fb2-6f21-4ab1-a4ef-a0e48947dbf1" xlink:href="bntx-20241231.xsd#bntx_LitigationSettlmentContributionToResearchAndDevelopment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialAssetsLineItems_50be2b30-1aa2-4e5c-80c9-c85a8efac08e" xlink:to="loc_bntx_LitigationSettlmentContributionToResearchAndDevelopment_199f2fb2-6f21-4ab1-a4ef-a0e48947dbf1" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofFairValuesofFinancialInstrumentsinFairValueLevel3Details" xlink:type="simple" xlink:href="bntx-20241231.xsd#FinancialAssetsandFinancialLiabilitiesSummaryofFairValuesofFinancialInstrumentsinFairValueLevel3Details"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofFairValuesofFinancialInstrumentsinFairValueLevel3Details" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_817d101b-6aab-4719-b531-2c193d4fa145" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfFinancialLiabilitiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_ddfe331f-4dbf-4109-8fd5-8ebdbfd70cc2" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfFinancialLiabilitiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_817d101b-6aab-4719-b531-2c193d4fa145" xlink:to="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_ddfe331f-4dbf-4109-8fd5-8ebdbfd70cc2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfLiabilitiesAxis_76c7bf4c-f8bc-4e90-a7fc-51fe47893804" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfLiabilitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_ddfe331f-4dbf-4109-8fd5-8ebdbfd70cc2" xlink:to="loc_ifrs-full_ClassesOfLiabilitiesAxis_76c7bf4c-f8bc-4e90-a7fc-51fe47893804" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfLiabilitiesDomain_76c7bf4c-f8bc-4e90-a7fc-51fe47893804_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfLiabilitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfLiabilitiesAxis_76c7bf4c-f8bc-4e90-a7fc-51fe47893804" xlink:to="loc_ifrs-full_ClassesOfLiabilitiesDomain_76c7bf4c-f8bc-4e90-a7fc-51fe47893804_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfLiabilitiesDomain_4e3eb0ca-181d-4f5e-8d7f-6e95d6744e2c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfLiabilitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfLiabilitiesAxis_76c7bf4c-f8bc-4e90-a7fc-51fe47893804" xlink:to="loc_ifrs-full_ClassesOfLiabilitiesDomain_4e3eb0ca-181d-4f5e-8d7f-6e95d6744e2c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ContingentConsiderationMember_8e9c7cc8-8983-48ad-8cf7-167086164e7e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ContingentConsiderationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfLiabilitiesDomain_4e3eb0ca-181d-4f5e-8d7f-6e95d6744e2c" xlink:to="loc_ifrs-full_ContingentConsiderationMember_8e9c7cc8-8983-48ad-8cf7-167086164e7e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LevelsOfFairValueHierarchyAxis_d2d394c5-3302-489d-876c-3ed19abf278f" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LevelsOfFairValueHierarchyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_ddfe331f-4dbf-4109-8fd5-8ebdbfd70cc2" xlink:to="loc_ifrs-full_LevelsOfFairValueHierarchyAxis_d2d394c5-3302-489d-876c-3ed19abf278f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_d2d394c5-3302-489d-876c-3ed19abf278f_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LevelsOfFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_LevelsOfFairValueHierarchyAxis_d2d394c5-3302-489d-876c-3ed19abf278f" xlink:to="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_d2d394c5-3302-489d-876c-3ed19abf278f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_0f9734d7-1b7c-449a-8be4-3ae0aa8b4af7" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LevelsOfFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_LevelsOfFairValueHierarchyAxis_d2d394c5-3302-489d-876c-3ed19abf278f" xlink:to="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_0f9734d7-1b7c-449a-8be4-3ae0aa8b4af7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Level3OfFairValueHierarchyMember_d9086867-be6a-4a41-9c5a-8579a8cca363" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_Level3OfFairValueHierarchyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_LevelsOfFairValueHierarchyDomain_0f9734d7-1b7c-449a-8be4-3ae0aa8b4af7" xlink:to="loc_ifrs-full_Level3OfFairValueHierarchyMember_d9086867-be6a-4a41-9c5a-8579a8cca363" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_UnobservableInputsAxis_68bc9d74-69af-4b0b-acfd-e315353ff2d4" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_UnobservableInputsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_ddfe331f-4dbf-4109-8fd5-8ebdbfd70cc2" xlink:to="loc_ifrs-full_UnobservableInputsAxis_68bc9d74-69af-4b0b-acfd-e315353ff2d4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_UnobservableInputsDomain_68bc9d74-69af-4b0b-acfd-e315353ff2d4_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_UnobservableInputsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_UnobservableInputsAxis_68bc9d74-69af-4b0b-acfd-e315353ff2d4" xlink:to="loc_ifrs-full_UnobservableInputsDomain_68bc9d74-69af-4b0b-acfd-e315353ff2d4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_UnobservableInputsDomain_9cdb0913-4187-4ef8-8c8e-3b5f7a9e80ba" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_UnobservableInputsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_UnobservableInputsAxis_68bc9d74-69af-4b0b-acfd-e315353ff2d4" xlink:to="loc_ifrs-full_UnobservableInputsDomain_9cdb0913-4187-4ef8-8c8e-3b5f7a9e80ba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CashFlowProjectionsMeasurementInputMember_7b69ea5f-746b-4b5d-abc5-ce3dc8216385" xlink:href="bntx-20241231.xsd#bntx_CashFlowProjectionsMeasurementInputMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_UnobservableInputsDomain_9cdb0913-4187-4ef8-8c8e-3b5f7a9e80ba" xlink:to="loc_bntx_CashFlowProjectionsMeasurementInputMember_7b69ea5f-746b-4b5d-abc5-ce3dc8216385" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DiscountRateMeasurementInputMember_a5e9b2a7-2f4d-4724-b952-91bf1d233ae6" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DiscountRateMeasurementInputMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_UnobservableInputsDomain_9cdb0913-4187-4ef8-8c8e-3b5f7a9e80ba" xlink:to="loc_ifrs-full_DiscountRateMeasurementInputMember_a5e9b2a7-2f4d-4724-b952-91bf1d233ae6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsAxis_cd9c30e3-0a52-4e93-967e-5fd790f7618a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_ddfe331f-4dbf-4109-8fd5-8ebdbfd70cc2" xlink:to="loc_ifrs-full_ClassesOfAssetsAxis_cd9c30e3-0a52-4e93-967e-5fd790f7618a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_cd9c30e3-0a52-4e93-967e-5fd790f7618a_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_cd9c30e3-0a52-4e93-967e-5fd790f7618a" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_cd9c30e3-0a52-4e93-967e-5fd790f7618a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_9b7e1767-96e4-413b-a456-286d4a2ecb7b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_cd9c30e3-0a52-4e93-967e-5fd790f7618a" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_9b7e1767-96e4-413b-a456-286d4a2ecb7b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_RoyaltyAssetsMember_2ac71f59-bada-4f14-8123-797f401f1931" xlink:href="bntx-20241231.xsd#bntx_RoyaltyAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_9b7e1767-96e4-413b-a456-286d4a2ecb7b" xlink:to="loc_bntx_RoyaltyAssetsMember_2ac71f59-bada-4f14-8123-797f401f1931" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantUnobservableInputLiabilities_e9481574-b288-4839-b8ae-98ff76fd46d2" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SignificantUnobservableInputLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_817d101b-6aab-4719-b531-2c193d4fa145" xlink:to="loc_ifrs-full_SignificantUnobservableInputLiabilities_e9481574-b288-4839-b8ae-98ff76fd46d2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseInFairValueMeasurementDueToChangeInMultipleUnobservableInputsToReflectReasonablyPossibleAlternativeAssumptionsLiabilities_a172465f-932a-43f5-b52c-ab2c68a861cd" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IncreaseInFairValueMeasurementDueToChangeInMultipleUnobservableInputsToReflectReasonablyPossibleAlternativeAssumptionsLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_817d101b-6aab-4719-b531-2c193d4fa145" xlink:to="loc_ifrs-full_IncreaseInFairValueMeasurementDueToChangeInMultipleUnobservableInputsToReflectReasonablyPossibleAlternativeAssumptionsLiabilities_a172465f-932a-43f5-b52c-ab2c68a861cd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DecreaseInFairValueMeasurementDueToChangeInMultipleUnobservableInputsToReflectReasonablyPossibleAlternativeAssumptionsLiabilities_3d182337-e10e-46a8-97e0-5136972e0fa3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DecreaseInFairValueMeasurementDueToChangeInMultipleUnobservableInputsToReflectReasonablyPossibleAlternativeAssumptionsLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_817d101b-6aab-4719-b531-2c193d4fa145" xlink:to="loc_ifrs-full_DecreaseInFairValueMeasurementDueToChangeInMultipleUnobservableInputsToReflectReasonablyPossibleAlternativeAssumptionsLiabilities_3d182337-e10e-46a8-97e0-5136972e0fa3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantUnobservableInputAssets_f21e1bdd-bea6-4468-b988-3f5c438608d6" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SignificantUnobservableInputAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_817d101b-6aab-4719-b531-2c193d4fa145" xlink:to="loc_ifrs-full_SignificantUnobservableInputAssets_f21e1bdd-bea6-4468-b988-3f5c438608d6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseInFairValueMeasurementDueToChangeInMultipleUnobservableInputsToReflectReasonablyPossibleAlternativeAssumptionsAssets_7227d278-a67a-4f34-8e10-318316a07377" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IncreaseInFairValueMeasurementDueToChangeInMultipleUnobservableInputsToReflectReasonablyPossibleAlternativeAssumptionsAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_817d101b-6aab-4719-b531-2c193d4fa145" xlink:to="loc_ifrs-full_IncreaseInFairValueMeasurementDueToChangeInMultipleUnobservableInputsToReflectReasonablyPossibleAlternativeAssumptionsAssets_7227d278-a67a-4f34-8e10-318316a07377" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DecreaseInFairValueMeasurementDueToChangeInMultipleUnobservableInputsToReflectReasonablyPossibleAlternativeAssumptionsAssets_e9ac83ca-1969-4527-a987-44e0ed4401b3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DecreaseInFairValueMeasurementDueToChangeInMultipleUnobservableInputsToReflectReasonablyPossibleAlternativeAssumptionsAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_817d101b-6aab-4719-b531-2c193d4fa145" xlink:to="loc_ifrs-full_DecreaseInFairValueMeasurementDueToChangeInMultipleUnobservableInputsToReflectReasonablyPossibleAlternativeAssumptionsAssets_e9ac83ca-1969-4527-a987-44e0ed4401b3" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofCarryingAmountofMonetaryAssetsDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#FinancialAssetsandFinancialLiabilitiesSummaryofCarryingAmountofMonetaryAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofCarryingAmountofMonetaryAssetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CashAndCashEquivalents1LineItems_4bbbe606-eb61-4c63-9533-0b5fc52abbfd" xlink:href="bntx-20241231.xsd#bntx_CashAndCashEquivalents1LineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CashAndCashEquivalentsTable_ed4840d1-ab43-4bab-bf7f-edba94fd3205" xlink:href="bntx-20241231.xsd#bntx_CashAndCashEquivalentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_bntx_CashAndCashEquivalents1LineItems_4bbbe606-eb61-4c63-9533-0b5fc52abbfd" xlink:to="loc_bntx_CashAndCashEquivalentsTable_ed4840d1-ab43-4bab-bf7f-edba94fd3205" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfRisksAxis_a3359a1a-1acf-4ab6-ba33-51b51a48bd12" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfRisksAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_CashAndCashEquivalentsTable_ed4840d1-ab43-4bab-bf7f-edba94fd3205" xlink:to="loc_ifrs-full_TypesOfRisksAxis_a3359a1a-1acf-4ab6-ba33-51b51a48bd12" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfRisksDomain_a3359a1a-1acf-4ab6-ba33-51b51a48bd12_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfRisksDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TypesOfRisksAxis_a3359a1a-1acf-4ab6-ba33-51b51a48bd12" xlink:to="loc_ifrs-full_TypesOfRisksDomain_a3359a1a-1acf-4ab6-ba33-51b51a48bd12_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfRisksDomain_6281da09-c291-4d22-9a83-d4911e799ad1" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfRisksDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TypesOfRisksAxis_a3359a1a-1acf-4ab6-ba33-51b51a48bd12" xlink:to="loc_ifrs-full_TypesOfRisksDomain_6281da09-c291-4d22-9a83-d4911e799ad1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrencyRiskMember_4a2f6597-330b-4bb0-b388-8cbfa1e96947" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CurrencyRiskMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfRisksDomain_6281da09-c291-4d22-9a83-d4911e799ad1" xlink:to="loc_ifrs-full_CurrencyRiskMember_4a2f6597-330b-4bb0-b388-8cbfa1e96947" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsAxis_d405b37e-8c39-462b-bb86-d9e9592e7c35" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_CashAndCashEquivalentsTable_ed4840d1-ab43-4bab-bf7f-edba94fd3205" xlink:to="loc_ifrs-full_ClassesOfAssetsAxis_d405b37e-8c39-462b-bb86-d9e9592e7c35" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_d405b37e-8c39-462b-bb86-d9e9592e7c35_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_d405b37e-8c39-462b-bb86-d9e9592e7c35" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_d405b37e-8c39-462b-bb86-d9e9592e7c35_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_b2fde659-fc7a-479f-8932-1a8d22d28977" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_d405b37e-8c39-462b-bb86-d9e9592e7c35" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_b2fde659-fc7a-479f-8932-1a8d22d28977" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CashAndCashEquivalents1Member_4e1efb14-dc7b-4f51-a369-d96a804708d7" xlink:href="bntx-20241231.xsd#bntx_CashAndCashEquivalents1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_b2fde659-fc7a-479f-8932-1a8d22d28977" xlink:to="loc_bntx_CashAndCashEquivalents1Member_4e1efb14-dc7b-4f51-a369-d96a804708d7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsAxis_43f99f2b-09b8-4a41-8d46-4f1aa5560dda" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_CashAndCashEquivalentsTable_ed4840d1-ab43-4bab-bf7f-edba94fd3205" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsAxis_43f99f2b-09b8-4a41-8d46-4f1aa5560dda" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_43f99f2b-09b8-4a41-8d46-4f1aa5560dda_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_43f99f2b-09b8-4a41-8d46-4f1aa5560dda" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_43f99f2b-09b8-4a41-8d46-4f1aa5560dda_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_e1909297-9615-4b5d-b8e9-fba2923b52ad" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_43f99f2b-09b8-4a41-8d46-4f1aa5560dda" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_e1909297-9615-4b5d-b8e9-fba2923b52ad" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_MonetaryAssetsMember_9105c8b2-25d8-491a-a6be-61153e6d5d35" xlink:href="bntx-20241231.xsd#bntx_MonetaryAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_e1909297-9615-4b5d-b8e9-fba2923b52ad" xlink:to="loc_bntx_MonetaryAssetsMember_9105c8b2-25d8-491a-a6be-61153e6d5d35" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialLiabilitiesAxis_81d8f1fc-1650-4b87-bdbf-78b453def9e1" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialLiabilitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_CashAndCashEquivalentsTable_ed4840d1-ab43-4bab-bf7f-edba94fd3205" xlink:to="loc_ifrs-full_ClassesOfFinancialLiabilitiesAxis_81d8f1fc-1650-4b87-bdbf-78b453def9e1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_81d8f1fc-1650-4b87-bdbf-78b453def9e1_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialLiabilitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialLiabilitiesAxis_81d8f1fc-1650-4b87-bdbf-78b453def9e1" xlink:to="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_81d8f1fc-1650-4b87-bdbf-78b453def9e1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_f5718a2c-a261-4e02-9c7d-cb3e1b02eb20" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfFinancialLiabilitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialLiabilitiesAxis_81d8f1fc-1650-4b87-bdbf-78b453def9e1" xlink:to="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_f5718a2c-a261-4e02-9c7d-cb3e1b02eb20" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_MonetaryLiabilityAndProvisionsMember_06b02741-1b82-45b9-96b5-2365bed97234" xlink:href="bntx-20241231.xsd#bntx_MonetaryLiabilityAndProvisionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_f5718a2c-a261-4e02-9c7d-cb3e1b02eb20" xlink:to="loc_bntx_MonetaryLiabilityAndProvisionsMember_06b02741-1b82-45b9-96b5-2365bed97234" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CashAndCashEquivalent_023772bb-b8a3-49f7-9699-96ac79823dc9" xlink:href="bntx-20241231.xsd#bntx_CashAndCashEquivalent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_CashAndCashEquivalents1LineItems_4bbbe606-eb61-4c63-9533-0b5fc52abbfd" xlink:to="loc_bntx_CashAndCashEquivalent_023772bb-b8a3-49f7-9699-96ac79823dc9" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofEffectofChangesinforeignExchangeRatesDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#FinancialAssetsandFinancialLiabilitiesSummaryofEffectofChangesinforeignExchangeRatesDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofEffectofChangesinforeignExchangeRatesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DisclosureOfEffectOfChangesInForeignExchangeRatesLineItems_37290db3-d036-4d8c-9869-0de69b289f7f" xlink:href="bntx-20241231.xsd#bntx_DisclosureOfEffectOfChangesInForeignExchangeRatesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DisclosureOfEffectOfChangesInForeignExchangeRatesTable_fb1999fc-659d-4667-83c8-115294f18fff" xlink:href="bntx-20241231.xsd#bntx_DisclosureOfEffectOfChangesInForeignExchangeRatesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_bntx_DisclosureOfEffectOfChangesInForeignExchangeRatesLineItems_37290db3-d036-4d8c-9869-0de69b289f7f" xlink:to="loc_bntx_DisclosureOfEffectOfChangesInForeignExchangeRatesTable_fb1999fc-659d-4667-83c8-115294f18fff" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_f5f27c2f-c4cd-4c1a-91c0-b82e74e60d7f" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfEffectOfChangesInForeignExchangeRatesTable_fb1999fc-659d-4667-83c8-115294f18fff" xlink:to="loc_srt_StatementGeographicalAxis_f5f27c2f-c4cd-4c1a-91c0-b82e74e60d7f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_f5f27c2f-c4cd-4c1a-91c0-b82e74e60d7f_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_f5f27c2f-c4cd-4c1a-91c0-b82e74e60d7f" xlink:to="loc_srt_SegmentGeographicalDomain_f5f27c2f-c4cd-4c1a-91c0-b82e74e60d7f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_09ec29d9-e257-414e-96d1-88c166be10dd" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_f5f27c2f-c4cd-4c1a-91c0-b82e74e60d7f" xlink:to="loc_srt_SegmentGeographicalDomain_09ec29d9-e257-414e-96d1-88c166be10dd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_69896ecb-d520-4939-a86d-d02d11497e20" xlink:href="https://xbrl.sec.gov/country/2024/country-2024.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_09ec29d9-e257-414e-96d1-88c166be10dd" xlink:to="loc_country_US_69896ecb-d520-4939-a86d-d02d11497e20" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CurrencyAxis_83d9b319-3e49-4704-9701-4a74602e73f8" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CurrencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfEffectOfChangesInForeignExchangeRatesTable_fb1999fc-659d-4667-83c8-115294f18fff" xlink:to="loc_srt_CurrencyAxis_83d9b319-3e49-4704-9701-4a74602e73f8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_currency_AllCurrenciesDomain_83d9b319-3e49-4704-9701-4a74602e73f8_default" xlink:href="https://xbrl.sec.gov/currency/2024/currency-2024.xsd#currency_AllCurrenciesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CurrencyAxis_83d9b319-3e49-4704-9701-4a74602e73f8" xlink:to="loc_currency_AllCurrenciesDomain_83d9b319-3e49-4704-9701-4a74602e73f8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_currency_AllCurrenciesDomain_b1b33b25-5ca7-4650-93b2-6b6e67abf25d" xlink:href="https://xbrl.sec.gov/currency/2024/currency-2024.xsd#currency_AllCurrenciesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CurrencyAxis_83d9b319-3e49-4704-9701-4a74602e73f8" xlink:to="loc_currency_AllCurrenciesDomain_b1b33b25-5ca7-4650-93b2-6b6e67abf25d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_currency_USD_06b8af26-c7df-4903-b22e-4c019f774fe2" xlink:href="https://xbrl.sec.gov/currency/2024/currency-2024.xsd#currency_USD"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_currency_AllCurrenciesDomain_b1b33b25-5ca7-4650-93b2-6b6e67abf25d" xlink:to="loc_currency_USD_06b8af26-c7df-4903-b22e-4c019f774fe2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfRisksAxis_dae9b41d-4706-4da3-811c-157a6653657e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfRisksAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfEffectOfChangesInForeignExchangeRatesTable_fb1999fc-659d-4667-83c8-115294f18fff" xlink:to="loc_ifrs-full_TypesOfRisksAxis_dae9b41d-4706-4da3-811c-157a6653657e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfRisksDomain_dae9b41d-4706-4da3-811c-157a6653657e_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfRisksDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TypesOfRisksAxis_dae9b41d-4706-4da3-811c-157a6653657e" xlink:to="loc_ifrs-full_TypesOfRisksDomain_dae9b41d-4706-4da3-811c-157a6653657e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfRisksDomain_fce1150a-18fd-4027-9b19-b11f8c9a2930" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfRisksDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TypesOfRisksAxis_dae9b41d-4706-4da3-811c-157a6653657e" xlink:to="loc_ifrs-full_TypesOfRisksDomain_fce1150a-18fd-4027-9b19-b11f8c9a2930" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrencyRiskMember_bfc43db0-cab5-4dd3-b260-cddb5215dc81" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CurrencyRiskMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfRisksDomain_fce1150a-18fd-4027-9b19-b11f8c9a2930" xlink:to="loc_ifrs-full_CurrencyRiskMember_bfc43db0-cab5-4dd3-b260-cddb5215dc81" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_HedgingInstrumentsAxis_95b32dd7-9c83-4ebb-912e-51e9fa22305a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_HedgingInstrumentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfEffectOfChangesInForeignExchangeRatesTable_fb1999fc-659d-4667-83c8-115294f18fff" xlink:to="loc_ifrs-full_HedgingInstrumentsAxis_95b32dd7-9c83-4ebb-912e-51e9fa22305a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_HedgingInstrumentsDomain_95b32dd7-9c83-4ebb-912e-51e9fa22305a_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_HedgingInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_HedgingInstrumentsAxis_95b32dd7-9c83-4ebb-912e-51e9fa22305a" xlink:to="loc_ifrs-full_HedgingInstrumentsDomain_95b32dd7-9c83-4ebb-912e-51e9fa22305a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_HedgingInstrumentsDomain_3a351a5b-2475-49f7-b544-e70e339901d3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_HedgingInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_HedgingInstrumentsAxis_95b32dd7-9c83-4ebb-912e-51e9fa22305a" xlink:to="loc_ifrs-full_HedgingInstrumentsDomain_3a351a5b-2475-49f7-b544-e70e339901d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PositiveFivePercentageChangeInForeignExchangeRateMember_5427f340-3381-4465-9639-9c17edb9678a" xlink:href="bntx-20241231.xsd#bntx_PositiveFivePercentageChangeInForeignExchangeRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_HedgingInstrumentsDomain_3a351a5b-2475-49f7-b544-e70e339901d3" xlink:to="loc_bntx_PositiveFivePercentageChangeInForeignExchangeRateMember_5427f340-3381-4465-9639-9c17edb9678a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NegativeFivePercentageChangeInForeignExchangeRateMember_44965b63-5801-4dd6-99a4-be6f511e522b" xlink:href="bntx-20241231.xsd#bntx_NegativeFivePercentageChangeInForeignExchangeRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_HedgingInstrumentsDomain_3a351a5b-2475-49f7-b544-e70e339901d3" xlink:to="loc_bntx_NegativeFivePercentageChangeInForeignExchangeRateMember_44965b63-5801-4dd6-99a4-be6f511e522b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClosingForeignExchangeRate_d25f9576-a25f-4539-9f4d-a628f2342807" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClosingForeignExchangeRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfEffectOfChangesInForeignExchangeRatesLineItems_37290db3-d036-4d8c-9869-0de69b289f7f" xlink:to="loc_ifrs-full_ClosingForeignExchangeRate_d25f9576-a25f-4539-9f4d-a628f2342807" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AverageForeignExchangeRate_0583e1f9-8e7f-408a-b84a-38c1b2114301" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AverageForeignExchangeRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfEffectOfChangesInForeignExchangeRatesLineItems_37290db3-d036-4d8c-9869-0de69b289f7f" xlink:to="loc_ifrs-full_AverageForeignExchangeRate_0583e1f9-8e7f-408a-b84a-38c1b2114301" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ForeignCurrencyChangesEffectOnLossBeforeTax_8b8e8f3e-1c4b-4e9b-9d34-2740e0cf2505" xlink:href="bntx-20241231.xsd#bntx_ForeignCurrencyChangesEffectOnLossBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfEffectOfChangesInForeignExchangeRatesLineItems_37290db3-d036-4d8c-9869-0de69b289f7f" xlink:to="loc_bntx_ForeignCurrencyChangesEffectOnLossBeforeTax_8b8e8f3e-1c4b-4e9b-9d34-2740e0cf2505" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ForeignCurrencyChangesEffectOnPretaxEquity_5389d241-6ba2-43d5-822a-a8225787402a" xlink:href="bntx-20241231.xsd#bntx_ForeignCurrencyChangesEffectOnPretaxEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfEffectOfChangesInForeignExchangeRatesLineItems_37290db3-d036-4d8c-9869-0de69b289f7f" xlink:to="loc_bntx_ForeignCurrencyChangesEffectOnPretaxEquity_5389d241-6ba2-43d5-822a-a8225787402a" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofMaturityProfileofFinancialLiabilitiesBasedonContractualUndiscountedPaymentsDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#FinancialAssetsandFinancialLiabilitiesSummaryofMaturityProfileofFinancialLiabilitiesBasedonContractualUndiscountedPaymentsDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofMaturityProfileofFinancialLiabilitiesBasedonContractualUndiscountedPaymentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_8fa5efd0-fe29-4034-a5d9-e7b4a0b8cb7b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfFinancialLiabilitiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_8431ca94-1eec-47ca-b9b1-0e8b30d2d1a5" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfFinancialLiabilitiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_8fa5efd0-fe29-4034-a5d9-e7b4a0b8cb7b" xlink:to="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_8431ca94-1eec-47ca-b9b1-0e8b30d2d1a5" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfRisksAxis_b904fdf3-c68f-46b2-9aa8-6c600ae739e3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfRisksAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_8431ca94-1eec-47ca-b9b1-0e8b30d2d1a5" xlink:to="loc_ifrs-full_TypesOfRisksAxis_b904fdf3-c68f-46b2-9aa8-6c600ae739e3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfRisksDomain_b904fdf3-c68f-46b2-9aa8-6c600ae739e3_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfRisksDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TypesOfRisksAxis_b904fdf3-c68f-46b2-9aa8-6c600ae739e3" xlink:to="loc_ifrs-full_TypesOfRisksDomain_b904fdf3-c68f-46b2-9aa8-6c600ae739e3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfRisksDomain_24c1ff9f-52dd-40d3-a2f5-75613778191d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfRisksDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TypesOfRisksAxis_b904fdf3-c68f-46b2-9aa8-6c600ae739e3" xlink:to="loc_ifrs-full_TypesOfRisksDomain_24c1ff9f-52dd-40d3-a2f5-75613778191d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LiquidityRiskMember_3a234679-901f-4060-ba7d-50aaf7ac1909" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LiquidityRiskMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfRisksDomain_24c1ff9f-52dd-40d3-a2f5-75613778191d" xlink:to="loc_ifrs-full_LiquidityRiskMember_3a234679-901f-4060-ba7d-50aaf7ac1909" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MaturityAxis_4336f177-26a7-4e49-ba30-47ca14584996" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MaturityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesTable_8431ca94-1eec-47ca-b9b1-0e8b30d2d1a5" xlink:to="loc_ifrs-full_MaturityAxis_4336f177-26a7-4e49-ba30-47ca14584996" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MaturityDomain_4336f177-26a7-4e49-ba30-47ca14584996_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MaturityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_MaturityAxis_4336f177-26a7-4e49-ba30-47ca14584996" xlink:to="loc_ifrs-full_MaturityDomain_4336f177-26a7-4e49-ba30-47ca14584996_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MaturityDomain_cbe8556b-efb7-45d0-8486-36627f51c59b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MaturityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_MaturityAxis_4336f177-26a7-4e49-ba30-47ca14584996" xlink:to="loc_ifrs-full_MaturityDomain_cbe8556b-efb7-45d0-8486-36627f51c59b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NotLaterThanOneYearMember_36f5445c-be9b-4cd6-b298-be4e1082a1ac" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NotLaterThanOneYearMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_cbe8556b-efb7-45d0-8486-36627f51c59b" xlink:to="loc_ifrs-full_NotLaterThanOneYearMember_36f5445c-be9b-4cd6-b298-be4e1082a1ac" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LaterThanTwoYearsAndNotLaterThanFiveYearsMember_559ad4df-55ab-40e6-8858-f8c8d0dd6847" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LaterThanTwoYearsAndNotLaterThanFiveYearsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_cbe8556b-efb7-45d0-8486-36627f51c59b" xlink:to="loc_ifrs-full_LaterThanTwoYearsAndNotLaterThanFiveYearsMember_559ad4df-55ab-40e6-8858-f8c8d0dd6847" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LaterThanFiveYearsMember_b549ce94-360a-4cbd-aa38-f99d0d5f1f9e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LaterThanFiveYearsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_cbe8556b-efb7-45d0-8486-36627f51c59b" xlink:to="loc_ifrs-full_LaterThanFiveYearsMember_b549ce94-360a-4cbd-aa38-f99d0d5f1f9e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_InterestBearingLoansAndBorrowings_942d35b1-3523-4459-8260-4248ecd262e2" xlink:href="bntx-20241231.xsd#bntx_InterestBearingLoansAndBorrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_8fa5efd0-fe29-4034-a5d9-e7b4a0b8cb7b" xlink:to="loc_bntx_InterestBearingLoansAndBorrowings_942d35b1-3523-4459-8260-4248ecd262e2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TradeAndOtherPayables_1330e06a-ce51-42d0-8663-4f7fbff96b7a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TradeAndOtherPayables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_8fa5efd0-fe29-4034-a5d9-e7b4a0b8cb7b" xlink:to="loc_ifrs-full_TradeAndOtherPayables_1330e06a-ce51-42d0-8663-4f7fbff96b7a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LeaseLiabilities_bf0aa66c-b063-4c92-b8ae-1b943c2e7a1a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LeaseLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_8fa5efd0-fe29-4034-a5d9-e7b4a0b8cb7b" xlink:to="loc_ifrs-full_LeaseLiabilities_bf0aa66c-b063-4c92-b8ae-1b943c2e7a1a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ContingentLiabilitiesRecognisedInBusinessCombination_3b41d4e3-f0ad-4555-bdb1-05604231f83d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ContingentLiabilitiesRecognisedInBusinessCombination"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_8fa5efd0-fe29-4034-a5d9-e7b4a0b8cb7b" xlink:to="loc_ifrs-full_ContingentLiabilitiesRecognisedInBusinessCombination_3b41d4e3-f0ad-4555-bdb1-05604231f83d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DerivativeFinancialLiabilities_db39cda0-821e-495c-bf64-a213a3223dbc" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DerivativeFinancialLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_8fa5efd0-fe29-4034-a5d9-e7b4a0b8cb7b" xlink:to="loc_ifrs-full_DerivativeFinancialLiabilities_db39cda0-821e-495c-bf64-a213a3223dbc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherFinancialLiabilities_eebac928-04cd-49ea-9045-6d61ea04ced9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_OtherFinancialLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_8fa5efd0-fe29-4034-a5d9-e7b4a0b8cb7b" xlink:to="loc_ifrs-full_OtherFinancialLiabilities_eebac928-04cd-49ea-9045-6d61ea04ced9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinancialLiabilities_58a10c39-35d4-411f-a14d-206d61625f51" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_FinancialLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfFinancialLiabilitiesLineItems_8fa5efd0-fe29-4034-a5d9-e7b4a0b8cb7b" xlink:to="loc_ifrs-full_FinancialLiabilities_58a10c39-35d4-411f-a14d-206d61625f51" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofChangesinLiabilitiesArisingFromFinancingActivitiesDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#FinancialAssetsandFinancialLiabilitiesSummaryofChangesinLiabilitiesArisingFromFinancingActivitiesDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/FinancialAssetsandFinancialLiabilitiesSummaryofChangesinLiabilitiesArisingFromFinancingActivitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfReconciliationOfLiabilitiesArisingFromFinancingActivitiesLineItems_bcddd993-7f7e-4408-a79b-e8f5dd61d27f" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfReconciliationOfLiabilitiesArisingFromFinancingActivitiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfReconciliationOfLiabilitiesArisingFromFinancingActivitiesTable_9e1cdeba-3807-450f-80aa-9e0dce23b9eb" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfReconciliationOfLiabilitiesArisingFromFinancingActivitiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfLiabilitiesArisingFromFinancingActivitiesLineItems_bcddd993-7f7e-4408-a79b-e8f5dd61d27f" xlink:to="loc_ifrs-full_DisclosureOfReconciliationOfLiabilitiesArisingFromFinancingActivitiesTable_9e1cdeba-3807-450f-80aa-9e0dce23b9eb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LiabilitiesArisingFromFinancingActivitiesAxis_8c1adcfc-ca52-4138-ab52-f6129c50c08a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LiabilitiesArisingFromFinancingActivitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfLiabilitiesArisingFromFinancingActivitiesTable_9e1cdeba-3807-450f-80aa-9e0dce23b9eb" xlink:to="loc_ifrs-full_LiabilitiesArisingFromFinancingActivitiesAxis_8c1adcfc-ca52-4138-ab52-f6129c50c08a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LiabilitiesArisingFromFinancingActivitiesDomain_8c1adcfc-ca52-4138-ab52-f6129c50c08a_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LiabilitiesArisingFromFinancingActivitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_LiabilitiesArisingFromFinancingActivitiesAxis_8c1adcfc-ca52-4138-ab52-f6129c50c08a" xlink:to="loc_ifrs-full_LiabilitiesArisingFromFinancingActivitiesDomain_8c1adcfc-ca52-4138-ab52-f6129c50c08a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LiabilitiesArisingFromFinancingActivitiesDomain_b80f5fc4-0a10-4cc4-8508-55f18e6b46d6" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LiabilitiesArisingFromFinancingActivitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_LiabilitiesArisingFromFinancingActivitiesAxis_8c1adcfc-ca52-4138-ab52-f6129c50c08a" xlink:to="loc_ifrs-full_LiabilitiesArisingFromFinancingActivitiesDomain_b80f5fc4-0a10-4cc4-8508-55f18e6b46d6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CurrentObligationsUnderLeaseContractsMember_a89a6b7b-46fa-483a-8670-8b145464347f" xlink:href="bntx-20241231.xsd#bntx_CurrentObligationsUnderLeaseContractsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_LiabilitiesArisingFromFinancingActivitiesDomain_b80f5fc4-0a10-4cc4-8508-55f18e6b46d6" xlink:to="loc_bntx_CurrentObligationsUnderLeaseContractsMember_a89a6b7b-46fa-483a-8670-8b145464347f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NoncurrentObligationsUnderLeaseContractsMember_e59f949d-b7a4-4270-9511-c1637767121b" xlink:href="bntx-20241231.xsd#bntx_NoncurrentObligationsUnderLeaseContractsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_LiabilitiesArisingFromFinancingActivitiesDomain_b80f5fc4-0a10-4cc4-8508-55f18e6b46d6" xlink:to="loc_bntx_NoncurrentObligationsUnderLeaseContractsMember_e59f949d-b7a4-4270-9511-c1637767121b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_InterestBearingLoansAndBorrowingsMember_e476c039-afb3-44d1-a675-ac90e8ca1a90" xlink:href="bntx-20241231.xsd#bntx_InterestBearingLoansAndBorrowingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_LiabilitiesArisingFromFinancingActivitiesDomain_b80f5fc4-0a10-4cc4-8508-55f18e6b46d6" xlink:to="loc_bntx_InterestBearingLoansAndBorrowingsMember_e476c039-afb3-44d1-a675-ac90e8ca1a90" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LiabilitiesArisingFromFinancingActivities_8b404cf6-06b3-4155-b00e-411b1ca1949b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LiabilitiesArisingFromFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfLiabilitiesArisingFromFinancingActivitiesLineItems_bcddd993-7f7e-4408-a79b-e8f5dd61d27f" xlink:to="loc_ifrs-full_LiabilitiesArisingFromFinancingActivities_8b404cf6-06b3-4155-b00e-411b1ca1949b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughFinancingCashFlowsLiabilitiesArisingFromFinancingActivities_df4dca7b-ea17-4f70-a554-36e7687004d1" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IncreaseDecreaseThroughFinancingCashFlowsLiabilitiesArisingFromFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfLiabilitiesArisingFromFinancingActivitiesLineItems_bcddd993-7f7e-4408-a79b-e8f5dd61d27f" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughFinancingCashFlowsLiabilitiesArisingFromFinancingActivities_df4dca7b-ea17-4f70-a554-36e7687004d1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseThroughNewLeasesLiabilitiesArisingFromFinancingActivities_cb862da1-8980-4841-9f6e-2bef4dc73b27" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IncreaseThroughNewLeasesLiabilitiesArisingFromFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfLiabilitiesArisingFromFinancingActivitiesLineItems_bcddd993-7f7e-4408-a79b-e8f5dd61d27f" xlink:to="loc_ifrs-full_IncreaseThroughNewLeasesLiabilitiesArisingFromFinancingActivities_cb862da1-8980-4841-9f6e-2bef4dc73b27" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_IncreaseDecreaseThroughReclassificationLiabilitiesArisingFromFinancingActivities_f2ec46e1-4298-482a-b219-ba45b12db7a8" xlink:href="bntx-20241231.xsd#bntx_IncreaseDecreaseThroughReclassificationLiabilitiesArisingFromFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfLiabilitiesArisingFromFinancingActivitiesLineItems_bcddd993-7f7e-4408-a79b-e8f5dd61d27f" xlink:to="loc_bntx_IncreaseDecreaseThroughReclassificationLiabilitiesArisingFromFinancingActivities_f2ec46e1-4298-482a-b219-ba45b12db7a8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughOtherChangesLiabilitiesArisingFromFinancingActivities_574ccc5f-56c0-41be-ab17-8f0501c08bee" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IncreaseDecreaseThroughOtherChangesLiabilitiesArisingFromFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReconciliationOfLiabilitiesArisingFromFinancingActivitiesLineItems_bcddd993-7f7e-4408-a79b-e8f5dd61d27f" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughOtherChangesLiabilitiesArisingFromFinancingActivities_574ccc5f-56c0-41be-ab17-8f0501c08bee" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LiabilitiesArisingFromFinancingActivities_b8fa6812-cbb6-4758-b0bc-b972a6c2104c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LiabilitiesArisingFromFinancingActivities"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/OtherNonFinancialAssetsAdditionalInformationDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#OtherNonFinancialAssetsAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/OtherNonFinancialAssetsAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_ec5ef8d6-326e-4896-b607-7a636afed7b1" xlink:href="bntx-20241231.xsd#bntx_DisclosureOfIncomeAndExpensesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DisclosureOfIncomeAndExpensesTable_413430f4-a62f-4f9c-9652-946e12347a08" xlink:href="bntx-20241231.xsd#bntx_DisclosureOfIncomeAndExpensesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_ec5ef8d6-326e-4896-b607-7a636afed7b1" xlink:to="loc_bntx_DisclosureOfIncomeAndExpensesTable_413430f4-a62f-4f9c-9652-946e12347a08" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MajorCustomersAxis_2e933b55-2fcc-43fb-96e0-882e59f34766" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MajorCustomersAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesTable_413430f4-a62f-4f9c-9652-946e12347a08" xlink:to="loc_ifrs-full_MajorCustomersAxis_2e933b55-2fcc-43fb-96e0-882e59f34766" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MajorCustomersDomain_2e933b55-2fcc-43fb-96e0-882e59f34766_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MajorCustomersDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_MajorCustomersAxis_2e933b55-2fcc-43fb-96e0-882e59f34766" xlink:to="loc_ifrs-full_MajorCustomersDomain_2e933b55-2fcc-43fb-96e0-882e59f34766_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MajorCustomersDomain_ce1c9069-cd54-4610-8f13-df02d0b98d5a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MajorCustomersDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_MajorCustomersAxis_2e933b55-2fcc-43fb-96e0-882e59f34766" xlink:to="loc_ifrs-full_MajorCustomersDomain_ce1c9069-cd54-4610-8f13-df02d0b98d5a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EuropeanCommissionToCollaborationPartnerMember_3d1b1eec-02ec-49cd-8a36-e984d3da9eb2" xlink:href="bntx-20241231.xsd#bntx_EuropeanCommissionToCollaborationPartnerMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MajorCustomersDomain_ce1c9069-cd54-4610-8f13-df02d0b98d5a" xlink:to="loc_bntx_EuropeanCommissionToCollaborationPartnerMember_3d1b1eec-02ec-49cd-8a36-e984d3da9eb2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInAssociatesAxis_8e196344-55d3-4825-8986-fc3fcad7ffa8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SignificantInvestmentsInAssociatesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesTable_413430f4-a62f-4f9c-9652-946e12347a08" xlink:to="loc_ifrs-full_SignificantInvestmentsInAssociatesAxis_8e196344-55d3-4825-8986-fc3fcad7ffa8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_8e196344-55d3-4825-8986-fc3fcad7ffa8_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SignificantInvestmentsInAssociatesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesAxis_8e196344-55d3-4825-8986-fc3fcad7ffa8" xlink:to="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_8e196344-55d3-4825-8986-fc3fcad7ffa8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_a51bb544-4796-40e4-9b65-ba2bea936886" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_SignificantInvestmentsInAssociatesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesAxis_8e196344-55d3-4825-8986-fc3fcad7ffa8" xlink:to="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_a51bb544-4796-40e4-9b65-ba2bea936886" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_RyvuTherapeuticsSAMember_e647f5bf-7991-492d-ab6e-b15e39a84d04" xlink:href="bntx-20241231.xsd#bntx_RyvuTherapeuticsSAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_a51bb544-4796-40e4-9b65-ba2bea936886" xlink:to="loc_bntx_RyvuTherapeuticsSAMember_e647f5bf-7991-492d-ab6e-b15e39a84d04" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_MediLinkTherapeuticsCo.LtdMember_3bc86615-ac15-4c48-8f2e-a11d78d8293b" xlink:href="bntx-20241231.xsd#bntx_MediLinkTherapeuticsCo.LtdMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_a51bb544-4796-40e4-9b65-ba2bea936886" xlink:to="loc_bntx_MediLinkTherapeuticsCo.LtdMember_3bc86615-ac15-4c48-8f2e-a11d78d8293b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis_d76ef35c-e051-4fd4-9db3-ef6335274a93" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesTable_413430f4-a62f-4f9c-9652-946e12347a08" xlink:to="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis_d76ef35c-e051-4fd4-9db3-ef6335274a93" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_d76ef35c-e051-4fd4-9db3-ef6335274a93_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis_d76ef35c-e051-4fd4-9db3-ef6335274a93" xlink:to="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_d76ef35c-e051-4fd4-9db3-ef6335274a93_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_fdb05b1e-d662-47fb-a24e-51dd19b3ca39" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis_d76ef35c-e051-4fd4-9db3-ef6335274a93" xlink:to="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_fdb05b1e-d662-47fb-a24e-51dd19b3ca39" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_InsuranceObligationAndServiceContractsMember_e59d52f8-b948-428d-937e-2de25979d93e" xlink:href="bntx-20241231.xsd#bntx_InsuranceObligationAndServiceContractsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_fdb05b1e-d662-47fb-a24e-51dd19b3ca39" xlink:to="loc_bntx_InsuranceObligationAndServiceContractsMember_e59d52f8-b948-428d-937e-2de25979d93e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_DeferredExpense_52593b12-cbc3-4eb0-9a19-6261af366989" xlink:href="bntx-20241231.xsd#bntx_DeferredExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_DisclosureOfIncomeAndExpensesLineItems_ec5ef8d6-326e-4896-b607-7a636afed7b1" xlink:to="loc_bntx_DeferredExpense_52593b12-cbc3-4eb0-9a19-6261af366989" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/IssuedCapitalandReservesAdditionalInformationDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#IssuedCapitalandReservesAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/IssuedCapitalandReservesAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_e51ac257-db73-4c7e-b432-d817982a9129" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfReservesWithinEquityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfReservesWithinEquityTable_964a1a7e-5508-4b2a-8b79-d02aaef64012" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfReservesWithinEquityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_e51ac257-db73-4c7e-b432-d817982a9129" xlink:to="loc_ifrs-full_DisclosureOfReservesWithinEquityTable_964a1a7e-5508-4b2a-8b79-d02aaef64012" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_47b95ff8-3ebd-4221-89fb-8f789b18b450" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityTable_964a1a7e-5508-4b2a-8b79-d02aaef64012" xlink:to="loc_srt_CounterpartyNameAxis_47b95ff8-3ebd-4221-89fb-8f789b18b450" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_47b95ff8-3ebd-4221-89fb-8f789b18b450_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_47b95ff8-3ebd-4221-89fb-8f789b18b450" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_47b95ff8-3ebd-4221-89fb-8f789b18b450_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_78f23862-0b85-47f2-b9fc-bfe33130601d" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_47b95ff8-3ebd-4221-89fb-8f789b18b450" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_78f23862-0b85-47f2-b9fc-bfe33130601d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PfizerIncMember_38c8a9e6-1e21-4b84-a85b-dc78780c58e0" xlink:href="bntx-20241231.xsd#bntx_PfizerIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_78f23862-0b85-47f2-b9fc-bfe33130601d" xlink:to="loc_bntx_PfizerIncMember_38c8a9e6-1e21-4b84-a85b-dc78780c58e0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComponentsOfEquityAxis_1a78068e-ebd3-4e07-8be7-6f3a8a017827" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ComponentsOfEquityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityTable_964a1a7e-5508-4b2a-8b79-d02aaef64012" xlink:to="loc_ifrs-full_ComponentsOfEquityAxis_1a78068e-ebd3-4e07-8be7-6f3a8a017827" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComponentsOfEquityDomain_1a78068e-ebd3-4e07-8be7-6f3a8a017827_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ComponentsOfEquityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ComponentsOfEquityAxis_1a78068e-ebd3-4e07-8be7-6f3a8a017827" xlink:to="loc_ifrs-full_ComponentsOfEquityDomain_1a78068e-ebd3-4e07-8be7-6f3a8a017827_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComponentsOfEquityDomain_47bbae4a-f1dd-46de-818f-efb054aac860" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ComponentsOfEquityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ComponentsOfEquityAxis_1a78068e-ebd3-4e07-8be7-6f3a8a017827" xlink:to="loc_ifrs-full_ComponentsOfEquityDomain_47bbae4a-f1dd-46de-818f-efb054aac860" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IssuedCapitalMember_85fe9c26-eab7-4e6a-98cf-8dc24c5e3889" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IssuedCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfEquityDomain_47bbae4a-f1dd-46de-818f-efb054aac860" xlink:to="loc_ifrs-full_IssuedCapitalMember_85fe9c26-eab7-4e6a-98cf-8dc24c5e3889" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CapitalReserveMember_c8b0aeab-3ea8-4e17-9f32-c0d73f56771c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CapitalReserveMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfEquityDomain_47bbae4a-f1dd-46de-818f-efb054aac860" xlink:to="loc_ifrs-full_CapitalReserveMember_c8b0aeab-3ea8-4e17-9f32-c0d73f56771c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_34e273b2-e3e3-4d4d-bc3b-81bb71f5c77d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityTable_964a1a7e-5508-4b2a-8b79-d02aaef64012" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_34e273b2-e3e3-4d4d-bc3b-81bb71f5c77d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_34e273b2-e3e3-4d4d-bc3b-81bb71f5c77d_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_34e273b2-e3e3-4d4d-bc3b-81bb71f5c77d" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_34e273b2-e3e3-4d4d-bc3b-81bb71f5c77d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_9c6f6600-c03c-4d6d-a712-306ae5b96ef5" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_34e273b2-e3e3-4d4d-bc3b-81bb71f5c77d" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_9c6f6600-c03c-4d6d-a712-306ae5b96ef5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_AnnouncementOfResearchDevelopmentAndCommercializationCollaborationAgreementMember_0fc46892-ef7e-479c-85a3-73a5dba5ec7d" xlink:href="bntx-20241231.xsd#bntx_AnnouncementOfResearchDevelopmentAndCommercializationCollaborationAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_9c6f6600-c03c-4d6d-a712-306ae5b96ef5" xlink:to="loc_bntx_AnnouncementOfResearchDevelopmentAndCommercializationCollaborationAgreementMember_0fc46892-ef7e-479c-85a3-73a5dba5ec7d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramPeriodAxis_0984cbcf-f006-4f9c-9dbc-ea3ba1ff7bbe" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramPeriodAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityTable_964a1a7e-5508-4b2a-8b79-d02aaef64012" xlink:to="loc_bntx_ShareRepurchaseProgramPeriodAxis_0984cbcf-f006-4f9c-9dbc-ea3ba1ff7bbe" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramPeriodDomain_0984cbcf-f006-4f9c-9dbc-ea3ba1ff7bbe_default" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_bntx_ShareRepurchaseProgramPeriodAxis_0984cbcf-f006-4f9c-9dbc-ea3ba1ff7bbe" xlink:to="loc_bntx_ShareRepurchaseProgramPeriodDomain_0984cbcf-f006-4f9c-9dbc-ea3ba1ff7bbe_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramPeriodDomain_db4e850a-bf1f-4bb2-81e2-b20f547bfbd6" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_bntx_ShareRepurchaseProgramPeriodAxis_0984cbcf-f006-4f9c-9dbc-ea3ba1ff7bbe" xlink:to="loc_bntx_ShareRepurchaseProgramPeriodDomain_db4e850a-bf1f-4bb2-81e2-b20f547bfbd6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramTrancheOneMember_3851455d-09eb-4cc4-884a-3b8b1fdd613b" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramTrancheOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_ShareRepurchaseProgramPeriodDomain_db4e850a-bf1f-4bb2-81e2-b20f547bfbd6" xlink:to="loc_bntx_ShareRepurchaseProgramTrancheOneMember_3851455d-09eb-4cc4-884a-3b8b1fdd613b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramTrancheTwoMember_c4584426-80e0-4d96-87bd-1878c5ad4a87" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramTrancheTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_ShareRepurchaseProgramPeriodDomain_db4e850a-bf1f-4bb2-81e2-b20f547bfbd6" xlink:to="loc_bntx_ShareRepurchaseProgramTrancheTwoMember_c4584426-80e0-4d96-87bd-1878c5ad4a87" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfSharesOutstanding_85e94800-43a5-40c5-92fc-f382e9768105" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_e51ac257-db73-4c7e-b432-d817982a9129" xlink:to="loc_ifrs-full_NumberOfSharesOutstanding_85e94800-43a5-40c5-92fc-f382e9768105" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NumberOfSharesHeldInTreasury_b2dcb947-0967-473a-bee5-00c6fb997323" xlink:href="bntx-20241231.xsd#bntx_NumberOfSharesHeldInTreasury"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_e51ac257-db73-4c7e-b432-d817982a9129" xlink:to="loc_bntx_NumberOfSharesHeldInTreasury_b2dcb947-0967-473a-bee5-00c6fb997323" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramAuthorizedAmount_808e1c77-2eda-4751-a86e-9475fbd77414" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramAuthorizedAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_e51ac257-db73-4c7e-b432-d817982a9129" xlink:to="loc_bntx_ShareRepurchaseProgramAuthorizedAmount_808e1c77-2eda-4751-a86e-9475fbd77414" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfSharesIssued_7c496b62-1cd7-43f0-9fe8-cdb0269e0cf4" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_e51ac257-db73-4c7e-b432-d817982a9129" xlink:to="loc_ifrs-full_NumberOfSharesIssued_7c496b62-1cd7-43f0-9fe8-cdb0269e0cf4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IssueOfEquity_2c2298aa-f22b-4775-abf6-f6b59a7ae3fe" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IssueOfEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_e51ac257-db73-4c7e-b432-d817982a9129" xlink:to="loc_ifrs-full_IssueOfEquity_2c2298aa-f22b-4775-abf6-f6b59a7ae3fe" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IssuedCapital_dfa1818b-ecc5-46ca-981f-601fe1f8b990" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_IssuedCapital"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_e51ac257-db73-4c7e-b432-d817982a9129" xlink:to="loc_ifrs-full_IssuedCapital_dfa1818b-ecc5-46ca-981f-601fe1f8b990" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AccumulatedChangesInFairValueOfFinancialAssetsRelatedCreditDerivativesOrSimilarInstruments_8d45ff8e-c1e7-4a47-9d5e-1e36e7fa0bdb" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AccumulatedChangesInFairValueOfFinancialAssetsRelatedCreditDerivativesOrSimilarInstruments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_e51ac257-db73-4c7e-b432-d817982a9129" xlink:to="loc_ifrs-full_AccumulatedChangesInFairValueOfFinancialAssetsRelatedCreditDerivativesOrSimilarInstruments_8d45ff8e-c1e7-4a47-9d5e-1e36e7fa0bdb" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DividendsProposedOrDeclaredBeforeFinancialStatementsAuthorisedForIssueButNotRecognisedAsDistributionToOwnersPerShare_f0678c99-1588-48da-86b7-1d1c723cf9bf" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DividendsProposedOrDeclaredBeforeFinancialStatementsAuthorisedForIssueButNotRecognisedAsDistributionToOwnersPerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_e51ac257-db73-4c7e-b432-d817982a9129" xlink:to="loc_ifrs-full_DividendsProposedOrDeclaredBeforeFinancialStatementsAuthorisedForIssueButNotRecognisedAsDistributionToOwnersPerShare_f0678c99-1588-48da-86b7-1d1c723cf9bf" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DividendsPaidClassifiedAsFinancingActivities_2ee9ded2-ff14-4724-a10e-7be249b083d4" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DividendsPaidClassifiedAsFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_e51ac257-db73-4c7e-b432-d817982a9129" xlink:to="loc_ifrs-full_DividendsPaidClassifiedAsFinancingActivities_2ee9ded2-ff14-4724-a10e-7be249b083d4" xlink:type="arc" order="8"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/IssuedCapitalandReservesShareRepurchaseProgramAuthorizedAmountDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#IssuedCapitalandReservesShareRepurchaseProgramAuthorizedAmountDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/IssuedCapitalandReservesShareRepurchaseProgramAuthorizedAmountDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_ad95b1c3-9d88-4c45-a715-2a0a434ff03d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfReservesWithinEquityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfReservesWithinEquityTable_33475a56-b0ba-437a-9b31-1093ccfe74db" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfReservesWithinEquityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_ad95b1c3-9d88-4c45-a715-2a0a434ff03d" xlink:to="loc_ifrs-full_DisclosureOfReservesWithinEquityTable_33475a56-b0ba-437a-9b31-1093ccfe74db" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramPeriodAxis_1e5c9bee-c34e-4509-ada3-eaaf1a60e8f8" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramPeriodAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityTable_33475a56-b0ba-437a-9b31-1093ccfe74db" xlink:to="loc_bntx_ShareRepurchaseProgramPeriodAxis_1e5c9bee-c34e-4509-ada3-eaaf1a60e8f8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramPeriodDomain_1e5c9bee-c34e-4509-ada3-eaaf1a60e8f8_default" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_bntx_ShareRepurchaseProgramPeriodAxis_1e5c9bee-c34e-4509-ada3-eaaf1a60e8f8" xlink:to="loc_bntx_ShareRepurchaseProgramPeriodDomain_1e5c9bee-c34e-4509-ada3-eaaf1a60e8f8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramPeriodDomain_8b7e0ecf-2844-4d74-a4c8-a95689a76ff4" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_bntx_ShareRepurchaseProgramPeriodAxis_1e5c9bee-c34e-4509-ada3-eaaf1a60e8f8" xlink:to="loc_bntx_ShareRepurchaseProgramPeriodDomain_8b7e0ecf-2844-4d74-a4c8-a95689a76ff4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramTrancheOneMember_6ccfcbc3-fac7-4e19-a86a-f2dad8b782b5" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramTrancheOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_ShareRepurchaseProgramPeriodDomain_8b7e0ecf-2844-4d74-a4c8-a95689a76ff4" xlink:to="loc_bntx_ShareRepurchaseProgramTrancheOneMember_6ccfcbc3-fac7-4e19-a86a-f2dad8b782b5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramTrancheTwoMember_dcbd0186-c6b8-42d4-b0a1-654dfe6bdf24" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramTrancheTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_ShareRepurchaseProgramPeriodDomain_8b7e0ecf-2844-4d74-a4c8-a95689a76ff4" xlink:to="loc_bntx_ShareRepurchaseProgramTrancheTwoMember_dcbd0186-c6b8-42d4-b0a1-654dfe6bdf24" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgram2023Member_07c2f514-9a96-4532-b734-a8e5b99839b4" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgram2023Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_ShareRepurchaseProgramPeriodDomain_8b7e0ecf-2844-4d74-a4c8-a95689a76ff4" xlink:to="loc_bntx_ShareRepurchaseProgram2023Member_07c2f514-9a96-4532-b734-a8e5b99839b4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramAuthorizedAmount_9d99fc23-af1e-4c82-8331-f515c3fa549c" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramAuthorizedAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_ad95b1c3-9d88-4c45-a715-2a0a434ff03d" xlink:to="loc_bntx_ShareRepurchaseProgramAuthorizedAmount_9d99fc23-af1e-4c82-8331-f515c3fa549c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramNumberOfAmericanDepositarySharesADSPurchased_b426f900-9277-485d-8a85-8e8fe4c81760" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramNumberOfAmericanDepositarySharesADSPurchased"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_ad95b1c3-9d88-4c45-a715-2a0a434ff03d" xlink:to="loc_bntx_ShareRepurchaseProgramNumberOfAmericanDepositarySharesADSPurchased_b426f900-9277-485d-8a85-8e8fe4c81760" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramAveragePricePerAmericanDepositaryShareADS_fe5d9819-09d4-40e7-a3f6-3de2c2245373" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramAveragePricePerAmericanDepositaryShareADS"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_ad95b1c3-9d88-4c45-a715-2a0a434ff03d" xlink:to="loc_bntx_ShareRepurchaseProgramAveragePricePerAmericanDepositaryShareADS_fe5d9819-09d4-40e7-a3f6-3de2c2245373" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareRepurchaseProgramNetAmountSpent_ff433ed2-9729-491a-95f8-26760db8aab9" xlink:href="bntx-20241231.xsd#bntx_ShareRepurchaseProgramNetAmountSpent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfReservesWithinEquityLineItems_ad95b1c3-9d88-4c45-a715-2a0a434ff03d" xlink:to="loc_bntx_ShareRepurchaseProgramNetAmountSpent_ff433ed2-9729-491a-95f8-26760db8aab9" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/ShareBasedPaymentsSummaryofExpenseRecognizedforEmployeeServicesReceivedDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#ShareBasedPaymentsSummaryofExpenseRecognizedforEmployeeServicesReceivedDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/ShareBasedPaymentsSummaryofExpenseRecognizedforEmployeeServicesReceivedDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_85b0f676-73a5-4909-8e62-725793297f31" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_3db21b75-c96c-4e40-8c9a-b5fcff85a568" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_85b0f676-73a5-4909-8e62-725793297f31" xlink:to="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_3db21b75-c96c-4e40-8c9a-b5fcff85a568" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_ec7c623a-b7a0-4a25-ba24-fe7b5ee62a78" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_3db21b75-c96c-4e40-8c9a-b5fcff85a568" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_ec7c623a-b7a0-4a25-ba24-fe7b5ee62a78" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_ec7c623a-b7a0-4a25-ba24-fe7b5ee62a78_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_ec7c623a-b7a0-4a25-ba24-fe7b5ee62a78" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_ec7c623a-b7a0-4a25-ba24-fe7b5ee62a78_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8d4f8837-bf2e-4847-87ad-91647a9916ff" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_ec7c623a-b7a0-4a25-ba24-fe7b5ee62a78" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8d4f8837-bf2e-4847-87ad-91647a9916ff" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOwnershipPlanMember_c25d6726-5889-4fe3-b38e-4986324d59a8" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOwnershipPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8d4f8837-bf2e-4847-87ad-91647a9916ff" xlink:to="loc_bntx_EmployeeStockOwnershipPlanMember_c25d6726-5889-4fe3-b38e-4986324d59a8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ChiefExecutiveOfficerGrantMember_a14f0c20-1ac1-43e2-a085-df11983f9f43" xlink:href="bntx-20241231.xsd#bntx_ChiefExecutiveOfficerGrantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8d4f8837-bf2e-4847-87ad-91647a9916ff" xlink:to="loc_bntx_ChiefExecutiveOfficerGrantMember_a14f0c20-1ac1-43e2-a085-df11983f9f43" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ManagementBoardGrantMember_4d71dea5-b76f-4e31-b416-6074fdee2a71" xlink:href="bntx-20241231.xsd#bntx_ManagementBoardGrantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8d4f8837-bf2e-4847-87ad-91647a9916ff" xlink:to="loc_bntx_ManagementBoardGrantMember_4d71dea5-b76f-4e31-b416-6074fdee2a71" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTechEmployeeEquityPlanForEmployeesBasedInEuropeMember_a4ef7beb-9f21-4567-bac5-2584b834cf1e" xlink:href="bntx-20241231.xsd#bntx_BioNTechEmployeeEquityPlanForEmployeesBasedInEuropeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8d4f8837-bf2e-4847-87ad-91647a9916ff" xlink:to="loc_bntx_BioNTechEmployeeEquityPlanForEmployeesBasedInEuropeMember_a4ef7beb-9f21-4567-bac5-2584b834cf1e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_InstaDeepEmployeeIncentivePlanMember_7744d068-99c8-4d1b-898c-39aa24e709ad" xlink:href="bntx-20241231.xsd#bntx_InstaDeepEmployeeIncentivePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8d4f8837-bf2e-4847-87ad-91647a9916ff" xlink:to="loc_bntx_InstaDeepEmployeeIncentivePlanMember_7744d068-99c8-4d1b-898c-39aa24e709ad" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTech2020RestrictedStockUnitPlanForNorthAmericaEmployeesMember_808d4beb-6d26-49f4-af6b-17c26b69f16d" xlink:href="bntx-20241231.xsd#bntx_BioNTech2020RestrictedStockUnitPlanForNorthAmericaEmployeesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8d4f8837-bf2e-4847-87ad-91647a9916ff" xlink:to="loc_bntx_BioNTech2020RestrictedStockUnitPlanForNorthAmericaEmployeesMember_808d4beb-6d26-49f4-af6b-17c26b69f16d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_A2024NorthAmericaEmployeeParticipationPlanMember_0e0a2e49-ec7d-4cc4-99b6-dee240a9ebbc" xlink:href="bntx-20241231.xsd#bntx_A2024NorthAmericaEmployeeParticipationPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8d4f8837-bf2e-4847-87ad-91647a9916ff" xlink:to="loc_bntx_A2024NorthAmericaEmployeeParticipationPlanMember_0e0a2e49-ec7d-4cc4-99b6-dee240a9ebbc" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RestrictedShareUnitsMember_968d7e2a-574f-4f4d-8b3b-ada537d87990" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RestrictedShareUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8d4f8837-bf2e-4847-87ad-91647a9916ff" xlink:to="loc_ifrs-full_RestrictedShareUnitsMember_968d7e2a-574f-4f4d-8b3b-ada537d87990" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis_e27b981a-179c-4b3b-a332-1a43cf591d40" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_3db21b75-c96c-4e40-8c9a-b5fcff85a568" xlink:to="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis_e27b981a-179c-4b3b-a332-1a43cf591d40" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_e27b981a-179c-4b3b-a332-1a43cf591d40_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis_e27b981a-179c-4b3b-a332-1a43cf591d40" xlink:to="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_e27b981a-179c-4b3b-a332-1a43cf591d40_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_f2165ae2-5a02-46dd-af98-9435dc636095" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis_e27b981a-179c-4b3b-a332-1a43cf591d40" xlink:to="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_f2165ae2-5a02-46dd-af98-9435dc636095" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CostOfSalesMember_c202b6ad-bfb9-44be-bcbc-ebe7bd11ab2a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_f2165ae2-5a02-46dd-af98-9435dc636095" xlink:to="loc_ifrs-full_CostOfSalesMember_c202b6ad-bfb9-44be-bcbc-ebe7bd11ab2a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ResearchAndDevelopmentExpensesMember_8b1da652-bb11-44c8-9d9d-3c0b34f9e049" xlink:href="bntx-20241231.xsd#bntx_ResearchAndDevelopmentExpensesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_f2165ae2-5a02-46dd-af98-9435dc636095" xlink:to="loc_bntx_ResearchAndDevelopmentExpensesMember_8b1da652-bb11-44c8-9d9d-3c0b34f9e049" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_SalesAndMarketingExpenseMember_76ea2a13-47bd-4d91-88bc-49a68156e4d1" xlink:href="bntx-20241231.xsd#bntx_SalesAndMarketingExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_f2165ae2-5a02-46dd-af98-9435dc636095" xlink:to="loc_bntx_SalesAndMarketingExpenseMember_76ea2a13-47bd-4d91-88bc-49a68156e4d1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_GeneralAndAdministrativeExpensesMember_514c8676-1371-4c43-af52-c5172336d2d9" xlink:href="bntx-20241231.xsd#bntx_GeneralAndAdministrativeExpensesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_f2165ae2-5a02-46dd-af98-9435dc636095" xlink:to="loc_bntx_GeneralAndAdministrativeExpensesMember_514c8676-1371-4c43-af52-c5172336d2d9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ExpenseFromEquitysettledSharebasedPaymentTransactionsInWhichGoodsOrServicesReceivedDidNotQualifyForRecognitionAsAssets_2b569d86-05ef-4854-9a25-c74a4ea834a5" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ExpenseFromEquitysettledSharebasedPaymentTransactionsInWhichGoodsOrServicesReceivedDidNotQualifyForRecognitionAsAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_85b0f676-73a5-4909-8e62-725793297f31" xlink:to="loc_ifrs-full_ExpenseFromEquitysettledSharebasedPaymentTransactionsInWhichGoodsOrServicesReceivedDidNotQualifyForRecognitionAsAssets_2b569d86-05ef-4854-9a25-c74a4ea834a5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ExpenseFromCashsettledSharebasedPaymentTransactionsInWhichGoodsOrServicesReceivedDidNotQualifyForRecognitionAsAssets_1528b664-6c47-4273-be84-f87d02966ec2" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ExpenseFromCashsettledSharebasedPaymentTransactionsInWhichGoodsOrServicesReceivedDidNotQualifyForRecognitionAsAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_85b0f676-73a5-4909-8e62-725793297f31" xlink:to="loc_ifrs-full_ExpenseFromCashsettledSharebasedPaymentTransactionsInWhichGoodsOrServicesReceivedDidNotQualifyForRecognitionAsAssets_1528b664-6c47-4273-be84-f87d02966ec2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ExpenseFromSharebasedPaymentTransactionsWithEmployees_e68d2edf-3b7c-4f83-90eb-b341e4440e17" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ExpenseFromSharebasedPaymentTransactionsWithEmployees"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_85b0f676-73a5-4909-8e62-725793297f31" xlink:to="loc_ifrs-full_ExpenseFromSharebasedPaymentTransactionsWithEmployees_e68d2edf-3b7c-4f83-90eb-b341e4440e17" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ReclassificationOfShareBasedPaymentArrangement_61ba0328-b239-4900-93ae-5164e15d1a19" xlink:href="bntx-20241231.xsd#bntx_ReclassificationOfShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_85b0f676-73a5-4909-8e62-725793297f31" xlink:to="loc_bntx_ReclassificationOfShareBasedPaymentArrangement_61ba0328-b239-4900-93ae-5164e15d1a19" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfOtherEquityInstrumentsExercisedOrVestedInSharebasedPaymentArrangement_d152da8b-478d-4520-b95c-b27394253692" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfOtherEquityInstrumentsExercisedOrVestedInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_85b0f676-73a5-4909-8e62-725793297f31" xlink:to="loc_ifrs-full_NumberOfOtherEquityInstrumentsExercisedOrVestedInSharebasedPaymentArrangement_d152da8b-478d-4520-b95c-b27394253692" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/ShareBasedPaymentsAdditionalInformationDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#ShareBasedPaymentsAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/ShareBasedPaymentsAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_23b662f0-4514-4fd9-8f4d-9b48cfa85bf7" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_23b662f0-4514-4fd9-8f4d-9b48cfa85bf7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_6734786d-1f0a-4f2b-a949-f0cb188902a6" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_23b662f0-4514-4fd9-8f4d-9b48cfa85bf7" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_6734786d-1f0a-4f2b-a949-f0cb188902a6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_6734786d-1f0a-4f2b-a949-f0cb188902a6_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_6734786d-1f0a-4f2b-a949-f0cb188902a6" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_6734786d-1f0a-4f2b-a949-f0cb188902a6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8910315f-6fed-410d-b947-19dc5a570acb" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_6734786d-1f0a-4f2b-a949-f0cb188902a6" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8910315f-6fed-410d-b947-19dc5a570acb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeEquityPlanLongTermIncentiveProgramMember_e8036560-f29c-4818-a0eb-1aea64128997" xlink:href="bntx-20241231.xsd#bntx_EmployeeEquityPlanLongTermIncentiveProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8910315f-6fed-410d-b947-19dc5a570acb" xlink:to="loc_bntx_EmployeeEquityPlanLongTermIncentiveProgramMember_e8036560-f29c-4818-a0eb-1aea64128997" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTech2024NorthAmericaEmployeeParticipationPlanRSUsMember_cc647b01-8238-4162-8725-64eae71f8096" xlink:href="bntx-20241231.xsd#bntx_BioNTech2024NorthAmericaEmployeeParticipationPlanRSUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8910315f-6fed-410d-b947-19dc5a570acb" xlink:to="loc_bntx_BioNTech2024NorthAmericaEmployeeParticipationPlanRSUsMember_cc647b01-8238-4162-8725-64eae71f8096" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTech2024NorthAmericaEmployeeParticipationPlanPSUsMember_d908837c-cd1f-4eaf-83d2-f9b75280324a" xlink:href="bntx-20241231.xsd#bntx_BioNTech2024NorthAmericaEmployeeParticipationPlanPSUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8910315f-6fed-410d-b947-19dc5a570acb" xlink:to="loc_bntx_BioNTech2024NorthAmericaEmployeeParticipationPlanPSUsMember_d908837c-cd1f-4eaf-83d2-f9b75280324a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_InstaDeepRSUProgramEmployeesMember_0f077930-b513-4a0c-b1ac-a3a7462dfef0" xlink:href="bntx-20241231.xsd#bntx_InstaDeepRSUProgramEmployeesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8910315f-6fed-410d-b947-19dc5a570acb" xlink:to="loc_bntx_InstaDeepRSUProgramEmployeesMember_0f077930-b513-4a0c-b1ac-a3a7462dfef0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_BioNTech2020RestrictedStockUnitPlanForNorthAmericaEmployeesMember_236f0fd7-bf4b-415c-8bb2-c363d7240e86" xlink:href="bntx-20241231.xsd#bntx_BioNTech2020RestrictedStockUnitPlanForNorthAmericaEmployeesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8910315f-6fed-410d-b947-19dc5a570acb" xlink:to="loc_bntx_BioNTech2020RestrictedStockUnitPlanForNorthAmericaEmployeesMember_236f0fd7-bf4b-415c-8bb2-c363d7240e86" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ManagementBoardGrantMember_685a9e4d-9da4-4c3f-8227-35041faebbaf" xlink:href="bntx-20241231.xsd#bntx_ManagementBoardGrantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8910315f-6fed-410d-b947-19dc5a570acb" xlink:to="loc_bntx_ManagementBoardGrantMember_685a9e4d-9da4-4c3f-8227-35041faebbaf" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ChiefExecutiveOfficerGrantMember_e9751c5e-7351-4b49-b7bc-c6e416df1ca8" xlink:href="bntx-20241231.xsd#bntx_ChiefExecutiveOfficerGrantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8910315f-6fed-410d-b947-19dc5a570acb" xlink:to="loc_bntx_ChiefExecutiveOfficerGrantMember_e9751c5e-7351-4b49-b7bc-c6e416df1ca8" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOwnershipPlanMember_fbc9bf3a-88e0-403d-9235-d1cb880e7010" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOwnershipPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8910315f-6fed-410d-b947-19dc5a570acb" xlink:to="loc_bntx_EmployeeStockOwnershipPlanMember_fbc9bf3a-88e0-403d-9235-d1cb880e7010" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeEquityPlanLongTermIncentivePlusProgramMember_2b61d8a0-2d56-4d08-b14e-d6a750473b7a" xlink:href="bntx-20241231.xsd#bntx_EmployeeEquityPlanLongTermIncentivePlusProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8910315f-6fed-410d-b947-19dc5a570acb" xlink:to="loc_bntx_EmployeeEquityPlanLongTermIncentivePlusProgramMember_2b61d8a0-2d56-4d08-b14e-d6a750473b7a" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CashSettledShareBasedPaymentArrangementMember_107b8a76-a65d-4f76-9c23-df9e764f344c" xlink:href="bntx-20241231.xsd#bntx_CashSettledShareBasedPaymentArrangementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8910315f-6fed-410d-b947-19dc5a570acb" xlink:to="loc_bntx_CashSettledShareBasedPaymentArrangementMember_107b8a76-a65d-4f76-9c23-df9e764f344c" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualAxis_34cdde01-4d9c-4fb8-9daf-a2a7c32c3779" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_23b662f0-4514-4fd9-8f4d-9b48cfa85bf7" xlink:to="loc_srt_TitleOfIndividualAxis_34cdde01-4d9c-4fb8-9daf-a2a7c32c3779" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_34cdde01-4d9c-4fb8-9daf-a2a7c32c3779_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_TitleOfIndividualAxis_34cdde01-4d9c-4fb8-9daf-a2a7c32c3779" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_34cdde01-4d9c-4fb8-9daf-a2a7c32c3779_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_890bcbea-3ab9-4f28-b775-0888541379a6" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_TitleOfIndividualAxis_34cdde01-4d9c-4fb8-9daf-a2a7c32c3779" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_890bcbea-3ab9-4f28-b775-0888541379a6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ProfessorUgurSahinMDMember_35618e95-4372-4e7e-acbc-a7592aa95956" xlink:href="bntx-20241231.xsd#bntx_ProfessorUgurSahinMDMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_890bcbea-3ab9-4f28-b775-0888541379a6" xlink:to="loc_bntx_ProfessorUgurSahinMDMember_35618e95-4372-4e7e-acbc-a7592aa95956" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_AllOtherMembersMember_8c9f6d5c-08ba-4a5a-9d19-91c0df87bb03" xlink:href="bntx-20241231.xsd#bntx_AllOtherMembersMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_890bcbea-3ab9-4f28-b775-0888541379a6" xlink:to="loc_bntx_AllOtherMembersMember_8c9f6d5c-08ba-4a5a-9d19-91c0df87bb03" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalAxis_6ad4b50a-26a4-494f-8cbb-032768deb584" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfShareCapitalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_23b662f0-4514-4fd9-8f4d-9b48cfa85bf7" xlink:to="loc_ifrs-full_ClassesOfShareCapitalAxis_6ad4b50a-26a4-494f-8cbb-032768deb584" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalDomain_6ad4b50a-26a4-494f-8cbb-032768deb584_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfShareCapitalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfShareCapitalAxis_6ad4b50a-26a4-494f-8cbb-032768deb584" xlink:to="loc_ifrs-full_ClassesOfShareCapitalDomain_6ad4b50a-26a4-494f-8cbb-032768deb584_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalDomain_7a3dfdcb-4892-422e-a354-9a4d36793eff" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfShareCapitalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfShareCapitalAxis_6ad4b50a-26a4-494f-8cbb-032768deb584" xlink:to="loc_ifrs-full_ClassesOfShareCapitalDomain_7a3dfdcb-4892-422e-a354-9a4d36793eff" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OrdinarySharesMember_7da1c589-697b-45fc-a88b-15f4b9d62449" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_OrdinarySharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfShareCapitalDomain_7a3dfdcb-4892-422e-a354-9a4d36793eff" xlink:to="loc_ifrs-full_OrdinarySharesMember_7da1c589-697b-45fc-a88b-15f4b9d62449" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_eff7c24f-8bb7-4a17-b24e-45bdc9fba0c5" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_23b662f0-4514-4fd9-8f4d-9b48cfa85bf7" xlink:to="loc_srt_RangeAxis_eff7c24f-8bb7-4a17-b24e-45bdc9fba0c5" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_eff7c24f-8bb7-4a17-b24e-45bdc9fba0c5_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_eff7c24f-8bb7-4a17-b24e-45bdc9fba0c5" xlink:to="loc_srt_RangeMember_eff7c24f-8bb7-4a17-b24e-45bdc9fba0c5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_afab4d0f-daad-4c11-935d-b3a192844110" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_eff7c24f-8bb7-4a17-b24e-45bdc9fba0c5" xlink:to="loc_srt_RangeMember_afab4d0f-daad-4c11-935d-b3a192844110" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_2a288f37-9040-4d8d-870b-8f7c1210932a" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_afab4d0f-daad-4c11-935d-b3a192844110" xlink:to="loc_srt_MaximumMember_2a288f37-9040-4d8d-870b-8f7c1210932a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_SaleOfStockAxis_c35b027d-5cb3-4a86-a05a-171ff36eb6ce" xlink:href="bntx-20241231.xsd#bntx_SaleOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_23b662f0-4514-4fd9-8f4d-9b48cfa85bf7" xlink:to="loc_bntx_SaleOfStockAxis_c35b027d-5cb3-4a86-a05a-171ff36eb6ce" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_SaleOfStockDomain_c35b027d-5cb3-4a86-a05a-171ff36eb6ce_default" xlink:href="bntx-20241231.xsd#bntx_SaleOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_bntx_SaleOfStockAxis_c35b027d-5cb3-4a86-a05a-171ff36eb6ce" xlink:to="loc_bntx_SaleOfStockDomain_c35b027d-5cb3-4a86-a05a-171ff36eb6ce_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_SaleOfStockDomain_c6ee89c0-4955-44fa-93f9-e9ec4f1d3ffd" xlink:href="bntx-20241231.xsd#bntx_SaleOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_bntx_SaleOfStockAxis_c35b027d-5cb3-4a86-a05a-171ff36eb6ce" xlink:to="loc_bntx_SaleOfStockDomain_c6ee89c0-4955-44fa-93f9-e9ec4f1d3ffd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOptionPlanMember_f7dc3ebf-7e68-46ac-a2d7-48aa8f89b182" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOptionPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_SaleOfStockDomain_c6ee89c0-4955-44fa-93f9-e9ec4f1d3ffd" xlink:to="loc_bntx_EmployeeStockOptionPlanMember_f7dc3ebf-7e68-46ac-a2d7-48aa8f89b182" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOwnershipPlanESOPNameAxis_91cb540a-dcb7-4305-9467-4258f10574e6" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOwnershipPlanESOPNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_23b662f0-4514-4fd9-8f4d-9b48cfa85bf7" xlink:to="loc_bntx_EmployeeStockOwnershipPlanESOPNameAxis_91cb540a-dcb7-4305-9467-4258f10574e6" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOwnershipPlanESOPNameDomain_91cb540a-dcb7-4305-9467-4258f10574e6_default" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOwnershipPlanESOPNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_bntx_EmployeeStockOwnershipPlanESOPNameAxis_91cb540a-dcb7-4305-9467-4258f10574e6" xlink:to="loc_bntx_EmployeeStockOwnershipPlanESOPNameDomain_91cb540a-dcb7-4305-9467-4258f10574e6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOwnershipPlanESOPNameDomain_554fae28-3ae8-41d7-a440-55c2e58afc88" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOwnershipPlanESOPNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_bntx_EmployeeStockOwnershipPlanESOPNameAxis_91cb540a-dcb7-4305-9467-4258f10574e6" xlink:to="loc_bntx_EmployeeStockOwnershipPlanESOPNameDomain_554fae28-3ae8-41d7-a440-55c2e58afc88" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOwnershipPlan2019Member_5ba188c7-4754-4cf9-98e7-86d22d45f97d" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOwnershipPlan2019Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_EmployeeStockOwnershipPlanESOPNameDomain_554fae28-3ae8-41d7-a440-55c2e58afc88" xlink:to="loc_bntx_EmployeeStockOwnershipPlan2019Member_5ba188c7-4754-4cf9-98e7-86d22d45f97d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareBasedPaymentsClassifiedAsOperatingActivities_96e97422-51ea-4f5d-b8bc-d690a3ed086d" xlink:href="bntx-20241231.xsd#bntx_ShareBasedPaymentsClassifiedAsOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_ShareBasedPaymentsClassifiedAsOperatingActivities_96e97422-51ea-4f5d-b8bc-d690a3ed086d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CashOutflowToTaxAuthority_d8b3a403-b9a5-495d-ad64-8d038d56a1d3" xlink:href="bntx-20241231.xsd#bntx_CashOutflowToTaxAuthority"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_CashOutflowToTaxAuthority_d8b3a403-b9a5-495d-ad64-8d038d56a1d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareBasedVestingPeriod_c625109e-a243-4c49-a15d-0426da3c2a30" xlink:href="bntx-20241231.xsd#bntx_ShareBasedVestingPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_ShareBasedVestingPeriod_c625109e-a243-4c49-a15d-0426da3c2a30" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfInstrumentsOtherEquityInstrumentsGranted_aafddc5b-b362-457b-b1d8-6bf690746c50" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfInstrumentsOtherEquityInstrumentsGranted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_NumberOfInstrumentsOtherEquityInstrumentsGranted_aafddc5b-b362-457b-b1d8-6bf690746c50" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_WeightedAverageExercisePriceOfOtherEquityInstrumentsGrantedInSharebasedPaymentArrangement2019_ddf3ab51-a5ac-4119-ba73-920dfbbb5d22" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_WeightedAverageExercisePriceOfOtherEquityInstrumentsGrantedInSharebasedPaymentArrangement2019"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_WeightedAverageExercisePriceOfOtherEquityInstrumentsGrantedInSharebasedPaymentArrangement2019_ddf3ab51-a5ac-4119-ba73-920dfbbb5d22" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfOtherEquityInstrumentsForfeitedInSharebasedPaymentArrangement_370cea2a-fc4c-43e3-8863-d4cddeb7e0ea" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfOtherEquityInstrumentsForfeitedInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_NumberOfOtherEquityInstrumentsForfeitedInSharebasedPaymentArrangement_370cea2a-fc4c-43e3-8863-d4cddeb7e0ea" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfOtherEquityInstrumentsOutstandingInSharebasedPaymentArrangement_78e6c07d-92ec-4779-80b6-61d6846f7a01" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfOtherEquityInstrumentsOutstandingInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_NumberOfOtherEquityInstrumentsOutstandingInSharebasedPaymentArrangement_78e6c07d-92ec-4779-80b6-61d6846f7a01" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_AnnualVestingPercentageOfShareBasedPaymentArrangement_a4b58c8c-344b-4638-a78a-3b838853848d" xlink:href="bntx-20241231.xsd#bntx_AnnualVestingPercentageOfShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_AnnualVestingPercentageOfShareBasedPaymentArrangement_a4b58c8c-344b-4638-a78a-3b838853848d" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareBasedCompensationTargetIncentiveAmount_4a2f900c-293e-40dd-b666-e8441b482cce" xlink:href="bntx-20241231.xsd#bntx_ShareBasedCompensationTargetIncentiveAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_ShareBasedCompensationTargetIncentiveAmount_4a2f900c-293e-40dd-b666-e8441b482cce" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_CashOutflowForShareBasedPaymentArrangementExercise_95a73663-964a-4bfa-b710-bbb5495ef935" xlink:href="bntx-20241231.xsd#bntx_CashOutflowForShareBasedPaymentArrangementExercise"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_CashOutflowForShareBasedPaymentArrangementExercise_95a73663-964a-4bfa-b710-bbb5495ef935" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LiabilitiesFromSharebasedPaymentTransactions2011_907700be-bd9a-4c08-a3c0-3cd79fd21b40" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LiabilitiesFromSharebasedPaymentTransactions2011"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_LiabilitiesFromSharebasedPaymentTransactions2011_907700be-bd9a-4c08-a3c0-3cd79fd21b40" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PercentageOfAmericanDepositorySharesToOrdinarySharesAwardsSettledAtEndOfMonth_6cef7e37-57a1-4787-889c-16f0b9b6663c" xlink:href="bntx-20241231.xsd#bntx_PercentageOfAmericanDepositorySharesToOrdinarySharesAwardsSettledAtEndOfMonth"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_PercentageOfAmericanDepositorySharesToOrdinarySharesAwardsSettledAtEndOfMonth_6cef7e37-57a1-4787-889c-16f0b9b6663c" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PercentageOfAmericanDepositorySharesToOrdinaryShares_b11a75d1-6ff7-47ed-91af-0197760c016f" xlink:href="bntx-20241231.xsd#bntx_PercentageOfAmericanDepositorySharesToOrdinaryShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_PercentageOfAmericanDepositorySharesToOrdinaryShares_b11a75d1-6ff7-47ed-91af-0197760c016f" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OptionTerm_45a079e5-6f62-4798-9c53-52e862f130b5" xlink:href="bntx-20241231.xsd#bntx_OptionTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_OptionTerm_45a079e5-6f62-4798-9c53-52e862f130b5" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OptionVestingPeriod_92c776aa-9989-4b38-a050-2d527b1d4fc3" xlink:href="bntx-20241231.xsd#bntx_OptionVestingPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_OptionVestingPeriod_92c776aa-9989-4b38-a050-2d527b1d4fc3" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OptionExercisablePeriod_b14f350b-c392-4810-9e05-110dbbfa533c" xlink:href="bntx-20241231.xsd#bntx_OptionExercisablePeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_OptionExercisablePeriod_b14f350b-c392-4810-9e05-110dbbfa533c" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PercentageOfIncreaseInExercisePrice_81186c1a-5f81-4d29-8b02-5b27a3c59755" xlink:href="bntx-20241231.xsd#bntx_PercentageOfIncreaseInExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_PercentageOfIncreaseInExercisePrice_81186c1a-5f81-4d29-8b02-5b27a3c59755" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ThresholdAmountOfDividendByNumberOfSharesOutstandingImmediatelyFollowingInitialPublicOffering_6e818756-8754-476b-a4ee-e2ef91947847" xlink:href="bntx-20241231.xsd#bntx_ThresholdAmountOfDividendByNumberOfSharesOutstandingImmediatelyFollowingInitialPublicOffering"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_ThresholdAmountOfDividendByNumberOfSharesOutstandingImmediatelyFollowingInitialPublicOffering_6e818756-8754-476b-a4ee-e2ef91947847" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PercentageOfTargetSharePriceApplicableForPriorTwelveMonth_fd703734-2286-41b0-a745-9be2838041eb" xlink:href="bntx-20241231.xsd#bntx_PercentageOfTargetSharePriceApplicableForPriorTwelveMonth"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_PercentageOfTargetSharePriceApplicableForPriorTwelveMonth_fd703734-2286-41b0-a745-9be2838041eb" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ReclassificationOfShareBasedPaymentArrangement_85ab2544-e56e-4bcd-b314-ed52ef9d8fb8" xlink:href="bntx-20241231.xsd#bntx_ReclassificationOfShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_ReclassificationOfShareBasedPaymentArrangement_85ab2544-e56e-4bcd-b314-ed52ef9d8fb8" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PriceOfAmericanDepositoryShareAsAPercentageOfExercisePriceMaximum_46261bdf-578c-49fe-92cf-b6feca2ab81f" xlink:href="bntx-20241231.xsd#bntx_PriceOfAmericanDepositoryShareAsAPercentageOfExercisePriceMaximum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_PriceOfAmericanDepositoryShareAsAPercentageOfExercisePriceMaximum_46261bdf-578c-49fe-92cf-b6feca2ab81f" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_MaximumEconomicBenefitsOfOptionsExercised_90cbd560-8c46-4a72-a3f8-53c95279f644" xlink:href="bntx-20241231.xsd#bntx_MaximumEconomicBenefitsOfOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_MaximumEconomicBenefitsOfOptionsExercised_90cbd560-8c46-4a72-a3f8-53c95279f644" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ExercisePriceShareOptionsGranted2019_37b52ae5-9628-4d82-b95d-12ee7243f465" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ExercisePriceShareOptionsGranted2019"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_ExercisePriceShareOptionsGranted2019_37b52ae5-9628-4d82-b95d-12ee7243f465" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_MaximumCashPaymentInShareBasedPaymentArrangement_b9ac9f94-626c-49c9-9edd-72a38625aa45" xlink:href="bntx-20241231.xsd#bntx_MaximumCashPaymentInShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_MaximumCashPaymentInShareBasedPaymentArrangement_b9ac9f94-626c-49c9-9edd-72a38625aa45" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_WeightedAverageRemainingContractualLifeOfOutstandingShareOptions2019_6b8a8c08-6a56-43d6-8173-17c50ec67e9d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_WeightedAverageRemainingContractualLifeOfOutstandingShareOptions2019"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_WeightedAverageRemainingContractualLifeOfOutstandingShareOptions2019_6b8a8c08-6a56-43d6-8173-17c50ec67e9d" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PhantomOptionAwardsLiability_3cc3fa4d-a3b2-4f94-a270-e2f51530af2c" xlink:href="bntx-20241231.xsd#bntx_PhantomOptionAwardsLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_PhantomOptionAwardsLiability_3cc3fa4d-a3b2-4f94-a270-e2f51530af2c" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfOtherEquityInstrumentsExercisedOrVestedInSharebasedPaymentArrangement_7b184311-36db-4cba-9b8e-3660468d26a0" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfOtherEquityInstrumentsExercisedOrVestedInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_NumberOfOtherEquityInstrumentsExercisedOrVestedInSharebasedPaymentArrangement_7b184311-36db-4cba-9b8e-3660468d26a0" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ExercisePriceCurrencyTranslationOfPublicOfferingPricePerShare_178848c2-b895-40f2-b226-97b49003883d" xlink:href="bntx-20241231.xsd#bntx_ExercisePriceCurrencyTranslationOfPublicOfferingPricePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_ExercisePriceCurrencyTranslationOfPublicOfferingPricePerShare_178848c2-b895-40f2-b226-97b49003883d" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_WeightedAverageSharePrice2019_e12e4763-f5f5-4010-b04f-23f2064ce741" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_WeightedAverageSharePrice2019"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_WeightedAverageSharePrice2019_e12e4763-f5f5-4010-b04f-23f2064ce741" xlink:type="arc" order="28"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisesInPeriodIntrinsicValue1_52a93072-fc45-4532-a0eb-ad04eca00773" xlink:href="bntx-20241231.xsd#bntx_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisesInPeriodIntrinsicValue1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisesInPeriodIntrinsicValue1_52a93072-fc45-4532-a0eb-ad04eca00773" xlink:type="arc" order="29"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PayablesOnSocialSecurityAndTaxesOtherThanIncomeTax_faecf8d5-d546-407e-82c3-4d584dc6a1ed" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_PayablesOnSocialSecurityAndTaxesOtherThanIncomeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_PayablesOnSocialSecurityAndTaxesOtherThanIncomeTax_faecf8d5-d546-407e-82c3-4d584dc6a1ed" xlink:type="arc" order="30"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfInstrumentsGrantedInSharebasedPaymentArrangement_b1b78952-cbec-486d-afde-8275332b21ac" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfInstrumentsGrantedInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_NumberOfInstrumentsGrantedInSharebasedPaymentArrangement_b1b78952-cbec-486d-afde-8275332b21ac" xlink:type="arc" order="31"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EffectiveExercisePriceMaximum_550ab857-e7e1-41d6-a05f-8eb2e9312cd0" xlink:href="bntx-20241231.xsd#bntx_EffectiveExercisePriceMaximum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_EffectiveExercisePriceMaximum_550ab857-e7e1-41d6-a05f-8eb2e9312cd0" xlink:type="arc" order="32"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ExerciseOfOptionRightsMinimumStrikePricePercent_ab8aa5e4-eb36-40b3-aa4c-3c85b82191d0" xlink:href="bntx-20241231.xsd#bntx_ExerciseOfOptionRightsMinimumStrikePricePercent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_ExerciseOfOptionRightsMinimumStrikePricePercent_ab8aa5e4-eb36-40b3-aa4c-3c85b82191d0" xlink:type="arc" order="33"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfShareOptionsExercisableInSharebasedPaymentArrangement_d8905b3e-7612-466d-a844-31c3db0a066f" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfShareOptionsExercisableInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_NumberOfShareOptionsExercisableInSharebasedPaymentArrangement_d8905b3e-7612-466d-a844-31c3db0a066f" xlink:type="arc" order="34"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ExercisePricePhantomOptionsGranted_5d149023-ecee-461e-ac9f-6cb4308094d9" xlink:href="bntx-20241231.xsd#bntx_ExercisePricePhantomOptionsGranted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_ExercisePricePhantomOptionsGranted_5d149023-ecee-461e-ac9f-6cb4308094d9" xlink:type="arc" order="35"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfShareOptionsExercisedInSharebasedPaymentArrangement_78f78478-e3f6-42c4-a209-4da6ddc3269e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfShareOptionsExercisedInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_NumberOfShareOptionsExercisedInSharebasedPaymentArrangement_78f78478-e3f6-42c4-a209-4da6ddc3269e" xlink:type="arc" order="36"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ExpenseFromSharebasedPaymentTransactionsInWhichGoodsOrServicesReceivedDidNotQualifyForRecognitionAsAssets_1cacebbd-d493-4a77-85e0-0a7833aaca7a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ExpenseFromSharebasedPaymentTransactionsInWhichGoodsOrServicesReceivedDidNotQualifyForRecognitionAsAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_ExpenseFromSharebasedPaymentTransactionsInWhichGoodsOrServicesReceivedDidNotQualifyForRecognitionAsAssets_1cacebbd-d493-4a77-85e0-0a7833aaca7a" xlink:type="arc" order="37"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfOutstandingShareOptions_17b61186-66d1-401a-8eac-2b43bb7a4798" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfOutstandingShareOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_NumberOfOutstandingShareOptions_17b61186-66d1-401a-8eac-2b43bb7a4798" xlink:type="arc" order="38"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareBasedPaymentArrangementRelatedToRightsVestedAndExercisable_d5f2431f-5896-4ade-9b0f-64f6fce32ff9" xlink:href="bntx-20241231.xsd#bntx_ShareBasedPaymentArrangementRelatedToRightsVestedAndExercisable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_bntx_ShareBasedPaymentArrangementRelatedToRightsVestedAndExercisable_d5f2431f-5896-4ade-9b0f-64f6fce32ff9" xlink:type="arc" order="39"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ExpenseFromSharebasedPaymentTransactionsWithEmployees_0455c75d-1b18-454f-a8bc-783cd53e56c3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ExpenseFromSharebasedPaymentTransactionsWithEmployees"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_f5431b4e-11a9-40e6-8be5-3693ee882b68" xlink:to="loc_ifrs-full_ExpenseFromSharebasedPaymentTransactionsWithEmployees_0455c75d-1b18-454f-a8bc-783cd53e56c3" xlink:type="arc" order="40"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/ShareBasedPaymentsReconciliationofOutstandingShareOptionsDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#ShareBasedPaymentsReconciliationofOutstandingShareOptionsDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/ShareBasedPaymentsReconciliationofOutstandingShareOptionsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_d118f452-10c1-4ecb-9460-5972eb02d127" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_e1f2a985-6670-463d-a240-911599da76be" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_d118f452-10c1-4ecb-9460-5972eb02d127" xlink:to="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_e1f2a985-6670-463d-a240-911599da76be" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_97ddbd08-88c6-4968-8c53-32a0e6f26ffa" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_e1f2a985-6670-463d-a240-911599da76be" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_97ddbd08-88c6-4968-8c53-32a0e6f26ffa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_97ddbd08-88c6-4968-8c53-32a0e6f26ffa_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_97ddbd08-88c6-4968-8c53-32a0e6f26ffa" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_97ddbd08-88c6-4968-8c53-32a0e6f26ffa_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_54150997-56dc-46be-9dde-3058f06a1cea" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_97ddbd08-88c6-4968-8c53-32a0e6f26ffa" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_54150997-56dc-46be-9dde-3058f06a1cea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeEquityPlanLongTermIncentivePlusProgramMember_c12e5d21-781a-4175-b3ed-777c35fe5161" xlink:href="bntx-20241231.xsd#bntx_EmployeeEquityPlanLongTermIncentivePlusProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_54150997-56dc-46be-9dde-3058f06a1cea" xlink:to="loc_bntx_EmployeeEquityPlanLongTermIncentivePlusProgramMember_c12e5d21-781a-4175-b3ed-777c35fe5161" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeEquityPlanLongTermIncentive2020ProgramMember_cb2430da-5db2-45d1-b88e-deab5b030d8c" xlink:href="bntx-20241231.xsd#bntx_EmployeeEquityPlanLongTermIncentive2020ProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_54150997-56dc-46be-9dde-3058f06a1cea" xlink:to="loc_bntx_EmployeeEquityPlanLongTermIncentive2020ProgramMember_cb2430da-5db2-45d1-b88e-deab5b030d8c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeEquityPlanLongTermIncentive2021ProgramMember_5cfbcbbf-84da-4de3-afb9-bf6202e16bcf" xlink:href="bntx-20241231.xsd#bntx_EmployeeEquityPlanLongTermIncentive2021ProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_54150997-56dc-46be-9dde-3058f06a1cea" xlink:to="loc_bntx_EmployeeEquityPlanLongTermIncentive2021ProgramMember_5cfbcbbf-84da-4de3-afb9-bf6202e16bcf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeEquityPlanLongTermIncentive2022ProgramMember_6f66f48b-a906-4be2-b34b-ee230f7e0b29" xlink:href="bntx-20241231.xsd#bntx_EmployeeEquityPlanLongTermIncentive2022ProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_54150997-56dc-46be-9dde-3058f06a1cea" xlink:to="loc_bntx_EmployeeEquityPlanLongTermIncentive2022ProgramMember_6f66f48b-a906-4be2-b34b-ee230f7e0b29" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeEquityPlanLongTermIncentiveProgramMember_eb1535c6-36a1-4993-ac76-55e3741a81b8" xlink:href="bntx-20241231.xsd#bntx_EmployeeEquityPlanLongTermIncentiveProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_54150997-56dc-46be-9dde-3058f06a1cea" xlink:to="loc_bntx_EmployeeEquityPlanLongTermIncentiveProgramMember_eb1535c6-36a1-4993-ac76-55e3741a81b8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOwnershipPlanMember_9710361f-4e47-40d0-a175-a4592b8cc37f" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOwnershipPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_54150997-56dc-46be-9dde-3058f06a1cea" xlink:to="loc_bntx_EmployeeStockOwnershipPlanMember_9710361f-4e47-40d0-a175-a4592b8cc37f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ShareOptionsOutstandingAbstract_70670e22-04f6-441a-b1eb-d40338c096dc" xlink:href="bntx-20241231.xsd#bntx_ShareOptionsOutstandingAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_d118f452-10c1-4ecb-9460-5972eb02d127" xlink:to="loc_bntx_ShareOptionsOutstandingAbstract_70670e22-04f6-441a-b1eb-d40338c096dc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfOutstandingShareOptions_ff7c8dec-59b1-4a09-965a-63ff286f33e3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfOutstandingShareOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_ShareOptionsOutstandingAbstract_70670e22-04f6-441a-b1eb-d40338c096dc" xlink:to="loc_ifrs-full_NumberOfOutstandingShareOptions_ff7c8dec-59b1-4a09-965a-63ff286f33e3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfShareOptionsForfeitedInSharebasedPaymentArrangement_b2a9ca85-b6cf-4515-899c-3df54fe1559c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfShareOptionsForfeitedInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_ShareOptionsOutstandingAbstract_70670e22-04f6-441a-b1eb-d40338c096dc" xlink:to="loc_ifrs-full_NumberOfShareOptionsForfeitedInSharebasedPaymentArrangement_b2a9ca85-b6cf-4515-899c-3df54fe1559c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfShareOptionsGrantedInSharebasedPaymentArrangement_bdee407c-d5af-4a62-9849-f8b7a0b8cddb" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfShareOptionsGrantedInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_ShareOptionsOutstandingAbstract_70670e22-04f6-441a-b1eb-d40338c096dc" xlink:to="loc_ifrs-full_NumberOfShareOptionsGrantedInSharebasedPaymentArrangement_bdee407c-d5af-4a62-9849-f8b7a0b8cddb" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NumberOfShareOptionsSettledInShareBasedPaymentArrangement_e66db519-7974-4360-bf2f-90502cc4ac8d" xlink:href="bntx-20241231.xsd#bntx_NumberOfShareOptionsSettledInShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_ShareOptionsOutstandingAbstract_70670e22-04f6-441a-b1eb-d40338c096dc" xlink:to="loc_bntx_NumberOfShareOptionsSettledInShareBasedPaymentArrangement_e66db519-7974-4360-bf2f-90502cc4ac8d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfShareOptionsExercisedInSharebasedPaymentArrangement_c29f019a-06af-47cf-aa96-f66688fa4513" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfShareOptionsExercisedInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_ShareOptionsOutstandingAbstract_70670e22-04f6-441a-b1eb-d40338c096dc" xlink:to="loc_ifrs-full_NumberOfShareOptionsExercisedInSharebasedPaymentArrangement_c29f019a-06af-47cf-aa96-f66688fa4513" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfOutstandingShareOptions_c2991d3c-61ac-4133-9a26-d3e6a48e1510" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfOutstandingShareOptions"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NumberOfShareOptionsVestedInShareBasedPaymentArrangement_ee87ab74-9c36-4687-8ccd-c7f46edfa84a" xlink:href="bntx-20241231.xsd#bntx_NumberOfShareOptionsVestedInShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_ShareOptionsOutstandingAbstract_70670e22-04f6-441a-b1eb-d40338c096dc" xlink:to="loc_bntx_NumberOfShareOptionsVestedInShareBasedPaymentArrangement_ee87ab74-9c36-4687-8ccd-c7f46edfa84a" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NumberOfShareOptionsUnestedInShareBasedPaymentArrangement_5214bde0-abab-471a-9e00-a92aeeb8c348" xlink:href="bntx-20241231.xsd#bntx_NumberOfShareOptionsUnestedInShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_ShareOptionsOutstandingAbstract_70670e22-04f6-441a-b1eb-d40338c096dc" xlink:to="loc_bntx_NumberOfShareOptionsUnestedInShareBasedPaymentArrangement_5214bde0-abab-471a-9e00-a92aeeb8c348" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsAbstract_c15f0350-efe9-4576-a0e6-ce6224ced530" xlink:href="bntx-20241231.xsd#bntx_NumberOfOrdinarySharesUnderlyingOptionsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_d118f452-10c1-4ecb-9460-5972eb02d127" xlink:to="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsAbstract_c15f0350-efe9-4576-a0e6-ce6224ced530" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsOutstanding_da99388b-d559-4292-8e31-96fe46b6cb13" xlink:href="bntx-20241231.xsd#bntx_NumberOfOrdinarySharesUnderlyingOptionsOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsAbstract_c15f0350-efe9-4576-a0e6-ce6224ced530" xlink:to="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsOutstanding_da99388b-d559-4292-8e31-96fe46b6cb13" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsForfeitedInShareBasedPaymentArrangement_eb5f02e6-b70d-4a59-bacf-604f06464b2d" xlink:href="bntx-20241231.xsd#bntx_NumberOfOrdinarySharesUnderlyingOptionsForfeitedInShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsAbstract_c15f0350-efe9-4576-a0e6-ce6224ced530" xlink:to="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsForfeitedInShareBasedPaymentArrangement_eb5f02e6-b70d-4a59-bacf-604f06464b2d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsExercisedInShareBasedPaymentArrangement_8ff5242b-5b9f-4376-92c7-e839a0e6938c" xlink:href="bntx-20241231.xsd#bntx_NumberOfOrdinarySharesUnderlyingOptionsExercisedInShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsAbstract_c15f0350-efe9-4576-a0e6-ce6224ced530" xlink:to="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsExercisedInShareBasedPaymentArrangement_8ff5242b-5b9f-4376-92c7-e839a0e6938c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsOutstanding_a817fd78-0b1a-4bb9-9e11-6f7ea70d3377" xlink:href="bntx-20241231.xsd#bntx_NumberOfOrdinarySharesUnderlyingOptionsOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsVestedInShareBasedPaymentArrangement_c2748145-529c-40b5-b45f-bc87e2f0ebd2" xlink:href="bntx-20241231.xsd#bntx_NumberOfOrdinarySharesUnderlyingOptionsVestedInShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsAbstract_c15f0350-efe9-4576-a0e6-ce6224ced530" xlink:to="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsVestedInShareBasedPaymentArrangement_c2748145-529c-40b5-b45f-bc87e2f0ebd2" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsUnvestedInShareBasedPaymentArrangement_23e0d66e-b3c3-48dd-b41c-f39d6a3b391b" xlink:href="bntx-20241231.xsd#bntx_NumberOfOrdinarySharesUnderlyingOptionsUnvestedInShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsAbstract_c15f0350-efe9-4576-a0e6-ce6224ced530" xlink:to="loc_bntx_NumberOfOrdinarySharesUnderlyingOptionsUnvestedInShareBasedPaymentArrangement_23e0d66e-b3c3-48dd-b41c-f39d6a3b391b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_WeightedAverageExercisePriceAbstract_8fc9eaff-b1a4-4716-b22a-a8e60706f840" xlink:href="bntx-20241231.xsd#bntx_WeightedAverageExercisePriceAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_d118f452-10c1-4ecb-9460-5972eb02d127" xlink:to="loc_bntx_WeightedAverageExercisePriceAbstract_8fc9eaff-b1a4-4716-b22a-a8e60706f840" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_WeightedAverageExercisePriceOfShareOptionsOutstandingInSharebasedPaymentArrangement2019_6e268742-5757-4463-8c63-f647cea0366b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_WeightedAverageExercisePriceOfShareOptionsOutstandingInSharebasedPaymentArrangement2019"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_WeightedAverageExercisePriceAbstract_8fc9eaff-b1a4-4716-b22a-a8e60706f840" xlink:to="loc_ifrs-full_WeightedAverageExercisePriceOfShareOptionsOutstandingInSharebasedPaymentArrangement2019_6e268742-5757-4463-8c63-f647cea0366b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_WeightedAverageExercisePriceOfShareOptionsForfeitedInSharebasedPaymentArrangement2019_ce5fbcc6-d1ad-4dbd-884e-61bb661b2186" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_WeightedAverageExercisePriceOfShareOptionsForfeitedInSharebasedPaymentArrangement2019"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_WeightedAverageExercisePriceAbstract_8fc9eaff-b1a4-4716-b22a-a8e60706f840" xlink:to="loc_ifrs-full_WeightedAverageExercisePriceOfShareOptionsForfeitedInSharebasedPaymentArrangement2019_ce5fbcc6-d1ad-4dbd-884e-61bb661b2186" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_WeightedAverageExercisePriceOfShareOptionsExercisedInShareBasedPaymentArrangement_b1eed0dc-6fec-444b-98ef-dfc5b4f8b10b" xlink:href="bntx-20241231.xsd#bntx_WeightedAverageExercisePriceOfShareOptionsExercisedInShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_WeightedAverageExercisePriceAbstract_8fc9eaff-b1a4-4716-b22a-a8e60706f840" xlink:to="loc_bntx_WeightedAverageExercisePriceOfShareOptionsExercisedInShareBasedPaymentArrangement_b1eed0dc-6fec-444b-98ef-dfc5b4f8b10b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_WeightedAverageExercisePriceOfShareOptionsOutstandingInSharebasedPaymentArrangement2019_8cb8f0b7-7767-4744-88de-1cd6fd8e867e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_WeightedAverageExercisePriceOfShareOptionsOutstandingInSharebasedPaymentArrangement2019"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_WeightedAverageExercisePriceOfShareOptionsVestedInShareBasedPaymentArrangement_fc912657-2b6b-4acd-8931-41fabee50dac" xlink:href="bntx-20241231.xsd#bntx_WeightedAverageExercisePriceOfShareOptionsVestedInShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_WeightedAverageExercisePriceAbstract_8fc9eaff-b1a4-4716-b22a-a8e60706f840" xlink:to="loc_bntx_WeightedAverageExercisePriceOfShareOptionsVestedInShareBasedPaymentArrangement_fc912657-2b6b-4acd-8931-41fabee50dac" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_WeightedAverageExercisePriceOfShareOptionsUnvestedInShareBasedPaymentArrangement_669901db-acb4-4bd4-9a01-e9dc473d7a0b" xlink:href="bntx-20241231.xsd#bntx_WeightedAverageExercisePriceOfShareOptionsUnvestedInShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_WeightedAverageExercisePriceAbstract_8fc9eaff-b1a4-4716-b22a-a8e60706f840" xlink:to="loc_bntx_WeightedAverageExercisePriceOfShareOptionsUnvestedInShareBasedPaymentArrangement_669901db-acb4-4bd4-9a01-e9dc473d7a0b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_WeightedAverageSharePrice2019_3a093ad9-b925-40c0-a122-24c55443bda7" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_WeightedAverageSharePrice2019"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_d118f452-10c1-4ecb-9460-5972eb02d127" xlink:to="loc_ifrs-full_WeightedAverageSharePrice2019_3a093ad9-b925-40c0-a122-24c55443bda7" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/ShareBasedPaymentsSummaryofInputsUsedinMeasurementofFairValueatGrantDateDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#ShareBasedPaymentsSummaryofInputsUsedinMeasurementofFairValueatGrantDateDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/ShareBasedPaymentsSummaryofInputsUsedinMeasurementofFairValueatGrantDateDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_909afeda-9ea0-4f4a-b47c-a9050464e9f5" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_a323b981-92ca-4919-872a-73dc7a9a573a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_909afeda-9ea0-4f4a-b47c-a9050464e9f5" xlink:to="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_a323b981-92ca-4919-872a-73dc7a9a573a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_7dd24b93-721b-4a76-9310-41408b3890b9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_a323b981-92ca-4919-872a-73dc7a9a573a" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_7dd24b93-721b-4a76-9310-41408b3890b9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_7dd24b93-721b-4a76-9310-41408b3890b9_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_7dd24b93-721b-4a76-9310-41408b3890b9" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_7dd24b93-721b-4a76-9310-41408b3890b9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8701961e-438d-4778-82a2-814ddd5c7a04" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_7dd24b93-721b-4a76-9310-41408b3890b9" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8701961e-438d-4778-82a2-814ddd5c7a04" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeEquityPlanLongTermIncentivePlusProgramMember_aced5e6a-7b4f-45d6-b36f-723d9489d529" xlink:href="bntx-20241231.xsd#bntx_EmployeeEquityPlanLongTermIncentivePlusProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8701961e-438d-4778-82a2-814ddd5c7a04" xlink:to="loc_bntx_EmployeeEquityPlanLongTermIncentivePlusProgramMember_aced5e6a-7b4f-45d6-b36f-723d9489d529" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeEquityPlanLongTermIncentive2020ProgramMember_195e6c5a-a404-4b95-8af1-1a9a41b033be" xlink:href="bntx-20241231.xsd#bntx_EmployeeEquityPlanLongTermIncentive2020ProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8701961e-438d-4778-82a2-814ddd5c7a04" xlink:to="loc_bntx_EmployeeEquityPlanLongTermIncentive2020ProgramMember_195e6c5a-a404-4b95-8af1-1a9a41b033be" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeEquityPlanLongTermIncentive2021ProgramMember_598b65c5-ebde-4207-8cc9-cf01a5c99f5b" xlink:href="bntx-20241231.xsd#bntx_EmployeeEquityPlanLongTermIncentive2021ProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8701961e-438d-4778-82a2-814ddd5c7a04" xlink:to="loc_bntx_EmployeeEquityPlanLongTermIncentive2021ProgramMember_598b65c5-ebde-4207-8cc9-cf01a5c99f5b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeEquityPlanLongTermIncentive2022ProgramMember_61a71a6b-fb75-4678-836a-b28d38ebebd2" xlink:href="bntx-20241231.xsd#bntx_EmployeeEquityPlanLongTermIncentive2022ProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8701961e-438d-4778-82a2-814ddd5c7a04" xlink:to="loc_bntx_EmployeeEquityPlanLongTermIncentive2022ProgramMember_61a71a6b-fb75-4678-836a-b28d38ebebd2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ManagementBoardGrantMember_3fb82e34-9c88-412f-8531-89f0434f3a05" xlink:href="bntx-20241231.xsd#bntx_ManagementBoardGrantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8701961e-438d-4778-82a2-814ddd5c7a04" xlink:to="loc_bntx_ManagementBoardGrantMember_3fb82e34-9c88-412f-8531-89f0434f3a05" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ChiefExecutiveOfficerGrantMember_f6c0d3c0-533b-4a03-88be-c8cb7a5a6436" xlink:href="bntx-20241231.xsd#bntx_ChiefExecutiveOfficerGrantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8701961e-438d-4778-82a2-814ddd5c7a04" xlink:to="loc_bntx_ChiefExecutiveOfficerGrantMember_f6c0d3c0-533b-4a03-88be-c8cb7a5a6436" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOwnershipPlanMember_31cc1df2-a911-49a9-8859-a82e6ce0c65e" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOwnershipPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_8701961e-438d-4778-82a2-814ddd5c7a04" xlink:to="loc_bntx_EmployeeStockOwnershipPlanMember_31cc1df2-a911-49a9-8859-a82e6ce0c65e" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_5c8d0f64-4644-4b2d-a1e6-19e2bd4a1bbc" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_a323b981-92ca-4919-872a-73dc7a9a573a" xlink:to="loc_srt_StatementScenarioAxis_5c8d0f64-4644-4b2d-a1e6-19e2bd4a1bbc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_5c8d0f64-4644-4b2d-a1e6-19e2bd4a1bbc_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_5c8d0f64-4644-4b2d-a1e6-19e2bd4a1bbc" xlink:to="loc_srt_ScenarioUnspecifiedDomain_5c8d0f64-4644-4b2d-a1e6-19e2bd4a1bbc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_e8e1948f-09b6-42f5-a6b4-f0fcb8c810e0" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_5c8d0f64-4644-4b2d-a1e6-19e2bd4a1bbc" xlink:to="loc_srt_ScenarioUnspecifiedDomain_e8e1948f-09b6-42f5-a6b4-f0fcb8c810e0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioForecastMember_20a1abb3-fe0d-45cc-a99b-324f1c6531a3" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ScenarioForecastMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ScenarioUnspecifiedDomain_e8e1948f-09b6-42f5-a6b4-f0fcb8c810e0" xlink:to="loc_srt_ScenarioForecastMember_20a1abb3-fe0d-45cc-a99b-324f1c6531a3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_0384d339-ba78-479b-b0c2-d92263d6c1d2" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_a323b981-92ca-4919-872a-73dc7a9a573a" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_0384d339-ba78-479b-b0c2-d92263d6c1d2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_0384d339-ba78-479b-b0c2-d92263d6c1d2_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_0384d339-ba78-479b-b0c2-d92263d6c1d2" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_0384d339-ba78-479b-b0c2-d92263d6c1d2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_4548bf63-06c6-4f18-b62b-d21f5b0d0f9a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_0384d339-ba78-479b-b0c2-d92263d6c1d2" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_4548bf63-06c6-4f18-b62b-d21f5b0d0f9a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EstimatedAllocationDateOneMember_7778fb58-06cc-4f9d-a3e6-cf6fc801161e" xlink:href="bntx-20241231.xsd#bntx_EstimatedAllocationDateOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_4548bf63-06c6-4f18-b62b-d21f5b0d0f9a" xlink:to="loc_bntx_EstimatedAllocationDateOneMember_7778fb58-06cc-4f9d-a3e6-cf6fc801161e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EstimatedAllocationDateTwoMember_bd44f2f2-067f-4f55-be2f-2511a1135dec" xlink:href="bntx-20241231.xsd#bntx_EstimatedAllocationDateTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_4548bf63-06c6-4f18-b62b-d21f5b0d0f9a" xlink:to="loc_bntx_EstimatedAllocationDateTwoMember_bd44f2f2-067f-4f55-be2f-2511a1135dec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EstimatedAllocationDateThreeMember_001c6443-8137-4866-92a5-11ca09598e18" xlink:href="bntx-20241231.xsd#bntx_EstimatedAllocationDateThreeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_4548bf63-06c6-4f18-b62b-d21f5b0d0f9a" xlink:to="loc_bntx_EstimatedAllocationDateThreeMember_001c6443-8137-4866-92a5-11ca09598e18" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EstimatedAllocationDateFourMember_6c088e25-92bd-462d-9b4e-64eee66ad4fc" xlink:href="bntx-20241231.xsd#bntx_EstimatedAllocationDateFourMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_4548bf63-06c6-4f18-b62b-d21f5b0d0f9a" xlink:to="loc_bntx_EstimatedAllocationDateFourMember_6c088e25-92bd-462d-9b4e-64eee66ad4fc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_WeightedAverageFairValueAtMeasurementDateShareOptionsGranted_389c1928-5b2c-41f6-a2cd-042b4599e646" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_WeightedAverageFairValueAtMeasurementDateShareOptionsGranted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_909afeda-9ea0-4f4a-b47c-a9050464e9f5" xlink:to="loc_ifrs-full_WeightedAverageFairValueAtMeasurementDateShareOptionsGranted_389c1928-5b2c-41f6-a2cd-042b4599e646" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_WeightedAverageSharePriceShareOptionsGranted2019_2de4f2e2-c663-4a2e-b3ac-06a6307287b8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_WeightedAverageSharePriceShareOptionsGranted2019"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_909afeda-9ea0-4f4a-b47c-a9050464e9f5" xlink:to="loc_ifrs-full_WeightedAverageSharePriceShareOptionsGranted2019_2de4f2e2-c663-4a2e-b3ac-06a6307287b8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ExercisePriceShareOptionsGranted2019_37ac80a3-3873-40be-8853-7e331cdaaaf2" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ExercisePriceShareOptionsGranted2019"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_909afeda-9ea0-4f4a-b47c-a9050464e9f5" xlink:to="loc_ifrs-full_ExercisePriceShareOptionsGranted2019_37ac80a3-3873-40be-8853-7e331cdaaaf2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DescriptionOfExpectedVolatilityShareOptionsGranted_5ab7ea6a-c387-4544-8e5f-0f8fe8781928" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DescriptionOfExpectedVolatilityShareOptionsGranted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_909afeda-9ea0-4f4a-b47c-a9050464e9f5" xlink:to="loc_ifrs-full_DescriptionOfExpectedVolatilityShareOptionsGranted_5ab7ea6a-c387-4544-8e5f-0f8fe8781928" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DescriptionOfOptionLifeShareOptionsGranted_588e6d46-8f04-496a-bda4-6b67f1ba8707" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DescriptionOfOptionLifeShareOptionsGranted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_909afeda-9ea0-4f4a-b47c-a9050464e9f5" xlink:to="loc_ifrs-full_DescriptionOfOptionLifeShareOptionsGranted_588e6d46-8f04-496a-bda4-6b67f1ba8707" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DescriptionOfRiskFreeInterestRateShareOptionsGranted_2117add8-4d73-49db-8d86-19a10da3d500" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DescriptionOfRiskFreeInterestRateShareOptionsGranted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_909afeda-9ea0-4f4a-b47c-a9050464e9f5" xlink:to="loc_ifrs-full_DescriptionOfRiskFreeInterestRateShareOptionsGranted_2117add8-4d73-49db-8d86-19a10da3d500" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/ShareBasedPaymentsScheduleofNumberandWeightedAverageExercisePriceofShareOptionsAllocatedandExpectedtobeAllocatedDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#ShareBasedPaymentsScheduleofNumberandWeightedAverageExercisePriceofShareOptionsAllocatedandExpectedtobeAllocatedDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/ShareBasedPaymentsScheduleofNumberandWeightedAverageExercisePriceofShareOptionsAllocatedandExpectedtobeAllocatedDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_49f3a962-6ab0-4a6c-8d61-bb340e7a42e9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_42aa2380-9857-4669-b253-9712b04e3104" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_49f3a962-6ab0-4a6c-8d61-bb340e7a42e9" xlink:to="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_42aa2380-9857-4669-b253-9712b04e3104" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_4163b149-2d57-42da-a2c2-296f81835d9b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_42aa2380-9857-4669-b253-9712b04e3104" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_4163b149-2d57-42da-a2c2-296f81835d9b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_4163b149-2d57-42da-a2c2-296f81835d9b_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_4163b149-2d57-42da-a2c2-296f81835d9b" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_4163b149-2d57-42da-a2c2-296f81835d9b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_ac363b6b-f36a-4fd7-84d6-fd41476ae0bb" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_4163b149-2d57-42da-a2c2-296f81835d9b" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_ac363b6b-f36a-4fd7-84d6-fd41476ae0bb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ManagementBoardGrantMember_52391cdd-1828-41d5-a138-37323dc4ef21" xlink:href="bntx-20241231.xsd#bntx_ManagementBoardGrantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_ac363b6b-f36a-4fd7-84d6-fd41476ae0bb" xlink:to="loc_bntx_ManagementBoardGrantMember_52391cdd-1828-41d5-a138-37323dc4ef21" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_1aea50d7-3b9c-4e70-86e8-3937f7755cff" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementTable_42aa2380-9857-4669-b253-9712b04e3104" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_1aea50d7-3b9c-4e70-86e8-3937f7755cff" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_1aea50d7-3b9c-4e70-86e8-3937f7755cff_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_1aea50d7-3b9c-4e70-86e8-3937f7755cff" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_1aea50d7-3b9c-4e70-86e8-3937f7755cff_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_3813540c-11e3-4a95-98e5-aaab60fbe82b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_1aea50d7-3b9c-4e70-86e8-3937f7755cff" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_3813540c-11e3-4a95-98e5-aaab60fbe82b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EstimatedAllocationDateOneMember_70dbebfd-944d-42ed-aead-de7822f86c33" xlink:href="bntx-20241231.xsd#bntx_EstimatedAllocationDateOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_3813540c-11e3-4a95-98e5-aaab60fbe82b" xlink:to="loc_bntx_EstimatedAllocationDateOneMember_70dbebfd-944d-42ed-aead-de7822f86c33" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EstimatedAllocationDateTwoMember_5f31b8d5-73e8-4195-93cb-8885a4469d47" xlink:href="bntx-20241231.xsd#bntx_EstimatedAllocationDateTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_3813540c-11e3-4a95-98e5-aaab60fbe82b" xlink:to="loc_bntx_EstimatedAllocationDateTwoMember_5f31b8d5-73e8-4195-93cb-8885a4469d47" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EstimatedAllocationDateThreeMember_10a8a431-79b0-401b-8336-06a0ba1b9d6d" xlink:href="bntx-20241231.xsd#bntx_EstimatedAllocationDateThreeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_3813540c-11e3-4a95-98e5-aaab60fbe82b" xlink:to="loc_bntx_EstimatedAllocationDateThreeMember_10a8a431-79b0-401b-8336-06a0ba1b9d6d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EstimatedAllocationDateFourMember_776c2f68-26a6-42b7-965b-8543e52553c8" xlink:href="bntx-20241231.xsd#bntx_EstimatedAllocationDateFourMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_3813540c-11e3-4a95-98e5-aaab60fbe82b" xlink:to="loc_bntx_EstimatedAllocationDateFourMember_776c2f68-26a6-42b7-965b-8543e52553c8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EstimatedAllocationDateFiveMember_541d80ea-b017-4a85-a662-4ee79672a520" xlink:href="bntx-20241231.xsd#bntx_EstimatedAllocationDateFiveMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_3813540c-11e3-4a95-98e5-aaab60fbe82b" xlink:to="loc_bntx_EstimatedAllocationDateFiveMember_541d80ea-b017-4a85-a662-4ee79672a520" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EstimatedAllocationDateSixMember_808cbcb7-d19a-4fc9-80ec-7c8b6fdf2f95" xlink:href="bntx-20241231.xsd#bntx_EstimatedAllocationDateSixMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_3813540c-11e3-4a95-98e5-aaab60fbe82b" xlink:to="loc_bntx_EstimatedAllocationDateSixMember_808cbcb7-d19a-4fc9-80ec-7c8b6fdf2f95" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfOutstandingShareOptions_77bb169b-4f69-4f41-b6d0-f8e5db077c32" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfOutstandingShareOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_49f3a962-6ab0-4a6c-8d61-bb340e7a42e9" xlink:to="loc_ifrs-full_NumberOfOutstandingShareOptions_77bb169b-4f69-4f41-b6d0-f8e5db077c32" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfShareOptionsGrantedInSharebasedPaymentArrangement_d96ff494-fb03-42ff-950b-4e62d309231a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfShareOptionsGrantedInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_49f3a962-6ab0-4a6c-8d61-bb340e7a42e9" xlink:to="loc_ifrs-full_NumberOfShareOptionsGrantedInSharebasedPaymentArrangement_d96ff494-fb03-42ff-950b-4e62d309231a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfShareOptionsExercisedInSharebasedPaymentArrangement_3268fc41-3b92-4d65-a7c4-5aac609cb3a7" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfShareOptionsExercisedInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_49f3a962-6ab0-4a6c-8d61-bb340e7a42e9" xlink:to="loc_ifrs-full_NumberOfShareOptionsExercisedInSharebasedPaymentArrangement_3268fc41-3b92-4d65-a7c4-5aac609cb3a7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfShareOptionsForfeitedInSharebasedPaymentArrangement_2fc1c906-2669-4acf-97f0-abd7b5190965" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfShareOptionsForfeitedInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_49f3a962-6ab0-4a6c-8d61-bb340e7a42e9" xlink:to="loc_ifrs-full_NumberOfShareOptionsForfeitedInSharebasedPaymentArrangement_2fc1c906-2669-4acf-97f0-abd7b5190965" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NumberOfShareOptionsAllocatedAndVestedSubjectToPerformanceAndWaitingRequirementsInShareBasedPaymentArrangement_a2531a59-6b1e-435e-afbe-bff45f65a6b8" xlink:href="bntx-20241231.xsd#bntx_NumberOfShareOptionsAllocatedAndVestedSubjectToPerformanceAndWaitingRequirementsInShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_49f3a962-6ab0-4a6c-8d61-bb340e7a42e9" xlink:to="loc_bntx_NumberOfShareOptionsAllocatedAndVestedSubjectToPerformanceAndWaitingRequirementsInShareBasedPaymentArrangement_a2531a59-6b1e-435e-afbe-bff45f65a6b8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NumberOfShareOptionsAllocatedAndUnvestedInShareBasedPaymentArrangement_09063363-efa8-40f1-9e70-483448c522fb" xlink:href="bntx-20241231.xsd#bntx_NumberOfShareOptionsAllocatedAndUnvestedInShareBasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTermsAndConditionsOfSharebasedPaymentArrangementLineItems_49f3a962-6ab0-4a6c-8d61-bb340e7a42e9" xlink:to="loc_bntx_NumberOfShareOptionsAllocatedAndUnvestedInShareBasedPaymentArrangement_09063363-efa8-40f1-9e70-483448c522fb" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/ContingentliabilitiesandotherfinancialcommitmentsScheduleofOtherFinancialCommitmentsDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#ContingentliabilitiesandotherfinancialcommitmentsScheduleofOtherFinancialCommitmentsDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/ContingentliabilitiesandotherfinancialcommitmentsScheduleofOtherFinancialCommitmentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfContingentLiabilitiesLineItems_604a23f0-7700-413e-b5ed-26274d03df7a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfContingentLiabilitiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfContingentLiabilitiesTable_2fa3d5b3-bb46-407b-b575-2bcaf9bf8ce5" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfContingentLiabilitiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfContingentLiabilitiesLineItems_604a23f0-7700-413e-b5ed-26274d03df7a" xlink:to="loc_ifrs-full_DisclosureOfContingentLiabilitiesTable_2fa3d5b3-bb46-407b-b575-2bcaf9bf8ce5" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MaturityAxis_beacd86f-ad01-4f0a-8d17-ea557ddcd7a8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MaturityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfContingentLiabilitiesTable_2fa3d5b3-bb46-407b-b575-2bcaf9bf8ce5" xlink:to="loc_ifrs-full_MaturityAxis_beacd86f-ad01-4f0a-8d17-ea557ddcd7a8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MaturityDomain_beacd86f-ad01-4f0a-8d17-ea557ddcd7a8_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MaturityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_MaturityAxis_beacd86f-ad01-4f0a-8d17-ea557ddcd7a8" xlink:to="loc_ifrs-full_MaturityDomain_beacd86f-ad01-4f0a-8d17-ea557ddcd7a8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MaturityDomain_e3156bae-edcc-433e-b494-3252af046287" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_MaturityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_MaturityAxis_beacd86f-ad01-4f0a-8d17-ea557ddcd7a8" xlink:to="loc_ifrs-full_MaturityDomain_e3156bae-edcc-433e-b494-3252af046287" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NotLaterThanOneYearMember_f3a675f5-40a6-43fa-8d9a-af9a93b04f6d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NotLaterThanOneYearMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_e3156bae-edcc-433e-b494-3252af046287" xlink:to="loc_ifrs-full_NotLaterThanOneYearMember_f3a675f5-40a6-43fa-8d9a-af9a93b04f6d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LaterThanOneYearAndNotLaterThanFiveYearsMember_8ac8ff37-fc5a-4887-b947-2c4a12e8885e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LaterThanOneYearAndNotLaterThanFiveYearsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_e3156bae-edcc-433e-b494-3252af046287" xlink:to="loc_ifrs-full_LaterThanOneYearAndNotLaterThanFiveYearsMember_8ac8ff37-fc5a-4887-b947-2c4a12e8885e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LaterThanFiveYearsMember_f2696ce6-eeec-4704-879d-782f41091f83" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_LaterThanFiveYearsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_e3156bae-edcc-433e-b494-3252af046287" xlink:to="loc_ifrs-full_LaterThanFiveYearsMember_f2696ce6-eeec-4704-879d-782f41091f83" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ContractualCommitmentsForAcquisitionOfPropertyPlantAndEquipment_816db7bc-8014-4d41-bf90-4dca19142d89" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ContractualCommitmentsForAcquisitionOfPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfContingentLiabilitiesLineItems_604a23f0-7700-413e-b5ed-26274d03df7a" xlink:to="loc_ifrs-full_ContractualCommitmentsForAcquisitionOfPropertyPlantAndEquipment_816db7bc-8014-4d41-bf90-4dca19142d89" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ContractualCommitmentsForAcquisitionOfIntangibleAssets_65ea6ec8-bb41-4623-9886-889a1e6a7ec9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ContractualCommitmentsForAcquisitionOfIntangibleAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfContingentLiabilitiesLineItems_604a23f0-7700-413e-b5ed-26274d03df7a" xlink:to="loc_ifrs-full_ContractualCommitmentsForAcquisitionOfIntangibleAssets_65ea6ec8-bb41-4623-9886-889a1e6a7ec9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ContractualCapitalCommitments_9238b621-0089-441c-af52-0cdbee6660c9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ContractualCapitalCommitments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfContingentLiabilitiesLineItems_604a23f0-7700-413e-b5ed-26274d03df7a" xlink:to="loc_ifrs-full_ContractualCapitalCommitments_9238b621-0089-441c-af52-0cdbee6660c9" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/LeasesSummaryofRightofUseAssetswithinConsolidatedStatementsofFinancialPositionDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#LeasesSummaryofRightofUseAssetswithinConsolidatedStatementsofFinancialPositionDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/LeasesSummaryofRightofUseAssetswithinConsolidatedStatementsofFinancialPositionDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems_aea12d92-06ad-495a-85c9-d96b20869332" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsTable_1566a7a6-1d31-4655-8e23-9b0103a5fa3a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems_aea12d92-06ad-495a-85c9-d96b20869332" xlink:to="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsTable_1566a7a6-1d31-4655-8e23-9b0103a5fa3a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsAxis_45465506-f4ad-4067-9a5d-dab0651f37e9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsTable_1566a7a6-1d31-4655-8e23-9b0103a5fa3a" xlink:to="loc_ifrs-full_ClassesOfAssetsAxis_45465506-f4ad-4067-9a5d-dab0651f37e9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_45465506-f4ad-4067-9a5d-dab0651f37e9_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_45465506-f4ad-4067-9a5d-dab0651f37e9" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_45465506-f4ad-4067-9a5d-dab0651f37e9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_410542a2-0a6a-49af-b610-2fbba3692eba" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_45465506-f4ad-4067-9a5d-dab0651f37e9" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_410542a2-0a6a-49af-b610-2fbba3692eba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BuildingsMember_f5937326-ce1d-4cec-90dd-4e1afb4b3f53" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BuildingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_410542a2-0a6a-49af-b610-2fbba3692eba" xlink:to="loc_ifrs-full_BuildingsMember_f5937326-ce1d-4cec-90dd-4e1afb4b3f53" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ProductionFacilitiesMember_56eee654-7502-416d-851d-40c3e3ef17a2" xlink:href="bntx-20241231.xsd#bntx_ProductionFacilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_410542a2-0a6a-49af-b610-2fbba3692eba" xlink:to="loc_bntx_ProductionFacilitiesMember_56eee654-7502-416d-851d-40c3e3ef17a2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OtherOperatingEquipmentMember_a7fc25db-e63d-4e37-8f60-c7713b0d0d0f" xlink:href="bntx-20241231.xsd#bntx_OtherOperatingEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_410542a2-0a6a-49af-b610-2fbba3692eba" xlink:to="loc_bntx_OtherOperatingEquipmentMember_a7fc25db-e63d-4e37-8f60-c7713b0d0d0f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RightofuseAssets_4b1a6d8a-f691-4a92-850a-2c66baf62e5e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_RightofuseAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems_aea12d92-06ad-495a-85c9-d96b20869332" xlink:to="loc_ifrs-full_RightofuseAssets_4b1a6d8a-f691-4a92-850a-2c66baf62e5e" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/LeasessummaryofAmountsRecognizedinConsolidatedStatementofOperationsDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#LeasessummaryofAmountsRecognizedinConsolidatedStatementofOperationsDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/LeasessummaryofAmountsRecognizedinConsolidatedStatementofOperationsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems_b3166e8f-d24d-4da9-afc8-f6872a48c211" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsTable_f10b5d64-9c43-45f3-8b67-ee370089873f" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems_b3166e8f-d24d-4da9-afc8-f6872a48c211" xlink:to="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsTable_f10b5d64-9c43-45f3-8b67-ee370089873f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsAxis_2d070546-7c91-4011-8919-e0fd5582c1b3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsTable_f10b5d64-9c43-45f3-8b67-ee370089873f" xlink:to="loc_ifrs-full_ClassesOfAssetsAxis_2d070546-7c91-4011-8919-e0fd5582c1b3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_2d070546-7c91-4011-8919-e0fd5582c1b3_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_2d070546-7c91-4011-8919-e0fd5582c1b3" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_2d070546-7c91-4011-8919-e0fd5582c1b3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_3bc8ea91-f8ef-4d84-a66f-ecedcc1ee119" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_2d070546-7c91-4011-8919-e0fd5582c1b3" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_3bc8ea91-f8ef-4d84-a66f-ecedcc1ee119" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BuildingsMember_afa04cd0-b395-4e89-81b9-62502d92ef47" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_BuildingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_3bc8ea91-f8ef-4d84-a66f-ecedcc1ee119" xlink:to="loc_ifrs-full_BuildingsMember_afa04cd0-b395-4e89-81b9-62502d92ef47" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ProductionFacilitiesMember_78ca3eaa-250c-4bee-b72d-463cdf48936f" xlink:href="bntx-20241231.xsd#bntx_ProductionFacilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_3bc8ea91-f8ef-4d84-a66f-ecedcc1ee119" xlink:to="loc_bntx_ProductionFacilitiesMember_78ca3eaa-250c-4bee-b72d-463cdf48936f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_OtherOperatingEquipmentMember_3fb53940-a833-442f-90b8-ecd2a4a12e98" xlink:href="bntx-20241231.xsd#bntx_OtherOperatingEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_3bc8ea91-f8ef-4d84-a66f-ecedcc1ee119" xlink:to="loc_bntx_OtherOperatingEquipmentMember_3fb53940-a833-442f-90b8-ecd2a4a12e98" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DepreciationRightofuseAssets_f64f245a-ba08-4ae1-99ef-3cd29194d9ee" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DepreciationRightofuseAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems_b3166e8f-d24d-4da9-afc8-f6872a48c211" xlink:to="loc_ifrs-full_DepreciationRightofuseAssets_f64f245a-ba08-4ae1-99ef-3cd29194d9ee" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_InterestExpenseOnLeaseLiabilities_47e23621-3d20-4a76-9e07-d54f10a31dac" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_InterestExpenseOnLeaseLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems_b3166e8f-d24d-4da9-afc8-f6872a48c211" xlink:to="loc_ifrs-full_InterestExpenseOnLeaseLiabilities_47e23621-3d20-4a76-9e07-d54f10a31dac" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ExpenseRelatingToShortTermLeasesAndLeasesOfLowValueAssetsForWhichRecognitionExemptionHasBeenUsed_5a966738-39e2-4ea3-99ca-f1f22648fbfe" xlink:href="bntx-20241231.xsd#bntx_ExpenseRelatingToShortTermLeasesAndLeasesOfLowValueAssetsForWhichRecognitionExemptionHasBeenUsed"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems_b3166e8f-d24d-4da9-afc8-f6872a48c211" xlink:to="loc_bntx_ExpenseRelatingToShortTermLeasesAndLeasesOfLowValueAssetsForWhichRecognitionExemptionHasBeenUsed_5a966738-39e2-4ea3-99ca-f1f22648fbfe" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_LeaseExpensesRecognizedInProfitOrLoss_0c720f0b-a144-4067-8369-2403fe54c6b2" xlink:href="bntx-20241231.xsd#bntx_LeaseExpensesRecognizedInProfitOrLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfQuantitativeInformationAboutRightofuseAssetsLineItems_b3166e8f-d24d-4da9-afc8-f6872a48c211" xlink:to="loc_bntx_LeaseExpensesRecognizedInProfitOrLoss_0c720f0b-a144-4067-8369-2403fe54c6b2" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/RelatedPartyDisclosuresSummaryofCompensationofKeyManagementPersonnelDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#RelatedPartyDisclosuresSummaryofCompensationofKeyManagementPersonnelDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/RelatedPartyDisclosuresSummaryofCompensationofKeyManagementPersonnelDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_a0a5f801-d33d-4290-8cc0-61b5c8733143" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_cd0b263c-3acb-4bc8-b811-4c588c7a6df2" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_a0a5f801-d33d-4290-8cc0-61b5c8733143" xlink:to="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_cd0b263c-3acb-4bc8-b811-4c588c7a6df2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_97e1783e-4720-4883-84b1-eb3f042daa0f" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_cd0b263c-3acb-4bc8-b811-4c588c7a6df2" xlink:to="loc_srt_CounterpartyNameAxis_97e1783e-4720-4883-84b1-eb3f042daa0f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_97e1783e-4720-4883-84b1-eb3f042daa0f_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_97e1783e-4720-4883-84b1-eb3f042daa0f" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_97e1783e-4720-4883-84b1-eb3f042daa0f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_ad574c5a-96f4-41b7-acca-427db004166e" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_97e1783e-4720-4883-84b1-eb3f042daa0f" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_ad574c5a-96f4-41b7-acca-427db004166e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ManagementBoardMember_078b2720-2a08-4881-a9f1-f6ec03e0ed8b" xlink:href="bntx-20241231.xsd#bntx_ManagementBoardMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_ad574c5a-96f4-41b7-acca-427db004166e" xlink:to="loc_bntx_ManagementBoardMember_078b2720-2a08-4881-a9f1-f6ec03e0ed8b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_SupervisoryBoardMember_3985e046-bb5e-4372-aa04-8db7a2f819c3" xlink:href="bntx-20241231.xsd#bntx_SupervisoryBoardMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_ad574c5a-96f4-41b7-acca-427db004166e" xlink:to="loc_bntx_SupervisoryBoardMember_3985e046-bb5e-4372-aa04-8db7a2f819c3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualAxis_d538d43f-4a04-4560-b12c-0497c4c74c67" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_cd0b263c-3acb-4bc8-b811-4c588c7a6df2" xlink:to="loc_srt_TitleOfIndividualAxis_d538d43f-4a04-4560-b12c-0497c4c74c67" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_d538d43f-4a04-4560-b12c-0497c4c74c67_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_TitleOfIndividualAxis_d538d43f-4a04-4560-b12c-0497c4c74c67" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_d538d43f-4a04-4560-b12c-0497c4c74c67_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_2bee5232-2f4d-4169-9b53-475201af5cd6" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_TitleOfIndividualAxis_d538d43f-4a04-4560-b12c-0497c4c74c67" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_2bee5232-2f4d-4169-9b53-475201af5cd6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_JensHolsteinMember_20097b68-832a-4a8f-b388-b974a7161f27" xlink:href="bntx-20241231.xsd#bntx_JensHolsteinMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_2bee5232-2f4d-4169-9b53-475201af5cd6" xlink:to="loc_bntx_JensHolsteinMember_20097b68-832a-4a8f-b388-b974a7161f27" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_SeanMarettMember_aa5bea05-60c9-42f2-9c41-232796888ad3" xlink:href="bntx-20241231.xsd#bntx_SeanMarettMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_2bee5232-2f4d-4169-9b53-475201af5cd6" xlink:to="loc_bntx_SeanMarettMember_aa5bea05-60c9-42f2-9c41-232796888ad3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_KeyManagementPersonnelCompensationFixedCompensation_7222d0e7-2e63-4eec-b886-e0119c2209e3" xlink:href="bntx-20241231.xsd#bntx_KeyManagementPersonnelCompensationFixedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_a0a5f801-d33d-4290-8cc0-61b5c8733143" xlink:to="loc_bntx_KeyManagementPersonnelCompensationFixedCompensation_7222d0e7-2e63-4eec-b886-e0119c2209e3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_KeyManagementPersonnelCompensationFringeBenefits_0c333bb5-a8a4-4526-bc7f-1ea28a8ac953" xlink:href="bntx-20241231.xsd#bntx_KeyManagementPersonnelCompensationFringeBenefits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_a0a5f801-d33d-4290-8cc0-61b5c8733143" xlink:to="loc_bntx_KeyManagementPersonnelCompensationFringeBenefits_0c333bb5-a8a4-4526-bc7f-1ea28a8ac953" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_KeyManagementPersonnelCompensationShorttermEmployeeBenefits_3347da0e-463f-4a9f-a912-2bd1f3d9f9c1" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_KeyManagementPersonnelCompensationShorttermEmployeeBenefits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_a0a5f801-d33d-4290-8cc0-61b5c8733143" xlink:to="loc_ifrs-full_KeyManagementPersonnelCompensationShorttermEmployeeBenefits_3347da0e-463f-4a9f-a912-2bd1f3d9f9c1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_KeyManagementPersonnelCompensationShortTermEmployeeBenefitsSecondInstallment_99bfd722-616f-4c14-8213-7e972dc08006" xlink:href="bntx-20241231.xsd#bntx_KeyManagementPersonnelCompensationShortTermEmployeeBenefitsSecondInstallment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_a0a5f801-d33d-4290-8cc0-61b5c8733143" xlink:to="loc_bntx_KeyManagementPersonnelCompensationShortTermEmployeeBenefitsSecondInstallment_99bfd722-616f-4c14-8213-7e972dc08006" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_KeyManagementPersonnelCompensationOtherVariableCompensation_7301ec51-696e-46a1-85b5-5eedb3c37c34" xlink:href="bntx-20241231.xsd#bntx_KeyManagementPersonnelCompensationOtherVariableCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_a0a5f801-d33d-4290-8cc0-61b5c8733143" xlink:to="loc_bntx_KeyManagementPersonnelCompensationOtherVariableCompensation_7301ec51-696e-46a1-85b5-5eedb3c37c34" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_KeyManagementPersonnelCompensationSharebasedPayment_4de4c121-3166-442d-b3b8-940ba444f9c9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_KeyManagementPersonnelCompensationSharebasedPayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_a0a5f801-d33d-4290-8cc0-61b5c8733143" xlink:to="loc_ifrs-full_KeyManagementPersonnelCompensationSharebasedPayment_4de4c121-3166-442d-b3b8-940ba444f9c9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_KeyManagementPersonnelCompensation_6305985a-cec6-4c3c-821a-9afe64f6a01a" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_KeyManagementPersonnelCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_a0a5f801-d33d-4290-8cc0-61b5c8733143" xlink:to="loc_ifrs-full_KeyManagementPersonnelCompensation_6305985a-cec6-4c3c-821a-9afe64f6a01a" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_KeyManagementPersonnelCompensationPhantomSharesAwarded_8383ecd0-cd74-4596-b2d8-5c9485c3a844" xlink:href="bntx-20241231.xsd#bntx_KeyManagementPersonnelCompensationPhantomSharesAwarded"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_a0a5f801-d33d-4290-8cc0-61b5c8733143" xlink:to="loc_bntx_KeyManagementPersonnelCompensationPhantomSharesAwarded_8383ecd0-cd74-4596-b2d8-5c9485c3a844" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_KeyManagementPersonnelCompensationSigningBonus_f48c8639-6a69-4ab7-bf1b-f1c836c74051" xlink:href="bntx-20241231.xsd#bntx_KeyManagementPersonnelCompensationSigningBonus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_a0a5f801-d33d-4290-8cc0-61b5c8733143" xlink:to="loc_bntx_KeyManagementPersonnelCompensationSigningBonus_f48c8639-6a69-4ab7-bf1b-f1c836c74051" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_KeyManagementPersonnelCompensationSeverance_7af9edb0-04a5-466c-8fff-bc2c7f62032f" xlink:href="bntx-20241231.xsd#bntx_KeyManagementPersonnelCompensationSeverance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_a0a5f801-d33d-4290-8cc0-61b5c8733143" xlink:to="loc_bntx_KeyManagementPersonnelCompensationSeverance_7af9edb0-04a5-466c-8fff-bc2c7f62032f" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_KeyManagementPersonnelCompensationSeveranceAdditionalPayment_95feb429-635a-4533-83f2-66e9a0e1bf43" xlink:href="bntx-20241231.xsd#bntx_KeyManagementPersonnelCompensationSeveranceAdditionalPayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_a0a5f801-d33d-4290-8cc0-61b5c8733143" xlink:to="loc_bntx_KeyManagementPersonnelCompensationSeveranceAdditionalPayment_95feb429-635a-4533-83f2-66e9a0e1bf43" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_KeyManagementPersonnelCompensationConsultancyAgreementPayment_588cf3c4-8a3d-40d3-be01-128eed3c6366" xlink:href="bntx-20241231.xsd#bntx_KeyManagementPersonnelCompensationConsultancyAgreementPayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_a0a5f801-d33d-4290-8cc0-61b5c8733143" xlink:to="loc_bntx_KeyManagementPersonnelCompensationConsultancyAgreementPayment_588cf3c4-8a3d-40d3-be01-128eed3c6366" xlink:type="arc" order="11"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/RelatedPartyDisclosuresAdditionalInformationDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#RelatedPartyDisclosuresAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/RelatedPartyDisclosuresAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_b78d50c7-ae4d-482b-8103-bf597c15606c" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_2637096e-f1d3-466b-b35b-4dc48522d614" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_b78d50c7-ae4d-482b-8103-bf597c15606c" xlink:to="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_2637096e-f1d3-466b-b35b-4dc48522d614" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOwnershipPlanESOPNameAxis_d2b39b4b-c6d6-475a-a759-e885e144a7a0" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOwnershipPlanESOPNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_2637096e-f1d3-466b-b35b-4dc48522d614" xlink:to="loc_bntx_EmployeeStockOwnershipPlanESOPNameAxis_d2b39b4b-c6d6-475a-a759-e885e144a7a0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOwnershipPlanESOPNameDomain_d2b39b4b-c6d6-475a-a759-e885e144a7a0_default" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOwnershipPlanESOPNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_bntx_EmployeeStockOwnershipPlanESOPNameAxis_d2b39b4b-c6d6-475a-a759-e885e144a7a0" xlink:to="loc_bntx_EmployeeStockOwnershipPlanESOPNameDomain_d2b39b4b-c6d6-475a-a759-e885e144a7a0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOwnershipPlanESOPNameDomain_bec4effd-28db-4ae5-8e60-7db6e3947724" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOwnershipPlanESOPNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_bntx_EmployeeStockOwnershipPlanESOPNameAxis_d2b39b4b-c6d6-475a-a759-e885e144a7a0" xlink:to="loc_bntx_EmployeeStockOwnershipPlanESOPNameDomain_bec4effd-28db-4ae5-8e60-7db6e3947724" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOwnershipPlan2018Member_3b06b553-b9e9-4d8d-bfe2-57fab4ea2143" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOwnershipPlan2018Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_EmployeeStockOwnershipPlanESOPNameDomain_bec4effd-28db-4ae5-8e60-7db6e3947724" xlink:to="loc_bntx_EmployeeStockOwnershipPlan2018Member_3b06b553-b9e9-4d8d-bfe2-57fab4ea2143" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOwnershipPlan2019Member_2c1e1aed-9432-4bb4-a94d-8e06c8bc6df4" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOwnershipPlan2019Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_EmployeeStockOwnershipPlanESOPNameDomain_bec4effd-28db-4ae5-8e60-7db6e3947724" xlink:to="loc_bntx_EmployeeStockOwnershipPlan2019Member_2c1e1aed-9432-4bb4-a94d-8e06c8bc6df4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOwnershipPlan2020Member_389dc8ca-1dae-4c2e-ac0c-e85688c565ab" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOwnershipPlan2020Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_bntx_EmployeeStockOwnershipPlanESOPNameDomain_bec4effd-28db-4ae5-8e60-7db6e3947724" xlink:to="loc_bntx_EmployeeStockOwnershipPlan2020Member_389dc8ca-1dae-4c2e-ac0c-e85688c565ab" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_7860a855-0af3-4c14-aa66-b7cd9be9dadd" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_2637096e-f1d3-466b-b35b-4dc48522d614" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_7860a855-0af3-4c14-aa66-b7cd9be9dadd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_7860a855-0af3-4c14-aa66-b7cd9be9dadd_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_7860a855-0af3-4c14-aa66-b7cd9be9dadd" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_7860a855-0af3-4c14-aa66-b7cd9be9dadd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_c14cc5e0-7e10-496a-b9b5-2bc607974b81" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_TypesOfSharebasedPaymentArrangementsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsAxis_7860a855-0af3-4c14-aa66-b7cd9be9dadd" xlink:to="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_c14cc5e0-7e10-496a-b9b5-2bc607974b81" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_EmployeeStockOptionPlanMember_1b96d4ad-4ae6-4b2f-aa25-e3876e2e46d1" xlink:href="bntx-20241231.xsd#bntx_EmployeeStockOptionPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_c14cc5e0-7e10-496a-b9b5-2bc607974b81" xlink:to="loc_bntx_EmployeeStockOptionPlanMember_1b96d4ad-4ae6-4b2f-aa25-e3876e2e46d1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ChiefExecutiveOfficerGrantMember_ce737cc3-7f88-424d-8d37-759d287b6638" xlink:href="bntx-20241231.xsd#bntx_ChiefExecutiveOfficerGrantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfSharebasedPaymentArrangementsDomain_c14cc5e0-7e10-496a-b9b5-2bc607974b81" xlink:to="loc_bntx_ChiefExecutiveOfficerGrantMember_ce737cc3-7f88-424d-8d37-759d287b6638" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualAxis_90736130-38a7-423a-8b36-5ee918b529e0" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_2637096e-f1d3-466b-b35b-4dc48522d614" xlink:to="loc_srt_TitleOfIndividualAxis_90736130-38a7-423a-8b36-5ee918b529e0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_90736130-38a7-423a-8b36-5ee918b529e0_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_TitleOfIndividualAxis_90736130-38a7-423a-8b36-5ee918b529e0" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_90736130-38a7-423a-8b36-5ee918b529e0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_eab54c6c-a9fb-4874-9fd4-cddabf5e1a75" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_TitleOfIndividualAxis_90736130-38a7-423a-8b36-5ee918b529e0" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_eab54c6c-a9fb-4874-9fd4-cddabf5e1a75" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_SeanMarettMember_77edbfd8-9903-4fe5-b0c1-15ad8896d5b4" xlink:href="bntx-20241231.xsd#bntx_SeanMarettMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_eab54c6c-a9fb-4874-9fd4-cddabf5e1a75" xlink:to="loc_bntx_SeanMarettMember_77edbfd8-9903-4fe5-b0c1-15ad8896d5b4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalAxis_72965dd6-081b-4422-9d01-4716a91ae4da" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfShareCapitalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_2637096e-f1d3-466b-b35b-4dc48522d614" xlink:to="loc_ifrs-full_ClassesOfShareCapitalAxis_72965dd6-081b-4422-9d01-4716a91ae4da" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalDomain_72965dd6-081b-4422-9d01-4716a91ae4da_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfShareCapitalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfShareCapitalAxis_72965dd6-081b-4422-9d01-4716a91ae4da" xlink:to="loc_ifrs-full_ClassesOfShareCapitalDomain_72965dd6-081b-4422-9d01-4716a91ae4da_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalDomain_87da31c7-64d9-4796-8e08-cef4469260b8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ClassesOfShareCapitalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfShareCapitalAxis_72965dd6-081b-4422-9d01-4716a91ae4da" xlink:to="loc_ifrs-full_ClassesOfShareCapitalDomain_87da31c7-64d9-4796-8e08-cef4469260b8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OrdinarySharesMember_a4e76c79-a7c5-4d27-b70b-eb299c2a3d95" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_OrdinarySharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfShareCapitalDomain_87da31c7-64d9-4796-8e08-cef4469260b8" xlink:to="loc_ifrs-full_OrdinarySharesMember_a4e76c79-a7c5-4d27-b70b-eb299c2a3d95" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_4bb6e8a4-4b6c-4e24-98b9-56742fef0b12" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_2637096e-f1d3-466b-b35b-4dc48522d614" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_4bb6e8a4-4b6c-4e24-98b9-56742fef0b12" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_4bb6e8a4-4b6c-4e24-98b9-56742fef0b12_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_4bb6e8a4-4b6c-4e24-98b9-56742fef0b12" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_4bb6e8a4-4b6c-4e24-98b9-56742fef0b12_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_b0bcca43-16ac-4f57-810f-f843e92b2c34" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_4bb6e8a4-4b6c-4e24-98b9-56742fef0b12" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_b0bcca43-16ac-4f57-810f-f843e92b2c34" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherRelatedPartiesMember_67a0e293-643f-43cb-8ab0-a7c84445d3c8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_OtherRelatedPartiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_b0bcca43-16ac-4f57-810f-f843e92b2c34" xlink:to="loc_ifrs-full_OtherRelatedPartiesMember_67a0e293-643f-43cb-8ab0-a7c84445d3c8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfOtherEquityInstrumentsExercisedOrVestedInSharebasedPaymentArrangement_b872f3ab-5094-4828-9278-5f73dd41d062" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfOtherEquityInstrumentsExercisedOrVestedInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_b78d50c7-ae4d-482b-8103-bf597c15606c" xlink:to="loc_ifrs-full_NumberOfOtherEquityInstrumentsExercisedOrVestedInSharebasedPaymentArrangement_b872f3ab-5094-4828-9278-5f73dd41d062" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfShareOptionsExercisedInSharebasedPaymentArrangement_f0e3407d-2a37-480b-92e6-2cb7ba58c48b" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfShareOptionsExercisedInSharebasedPaymentArrangement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_b78d50c7-ae4d-482b-8103-bf597c15606c" xlink:to="loc_ifrs-full_NumberOfShareOptionsExercisedInSharebasedPaymentArrangement_f0e3407d-2a37-480b-92e6-2cb7ba58c48b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_NumberOfShareOptionsHeld_23daf2e4-b8b4-4e2c-b457-a9a7db4c794c" xlink:href="bntx-20241231.xsd#bntx_NumberOfShareOptionsHeld"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_b78d50c7-ae4d-482b-8103-bf597c15606c" xlink:to="loc_bntx_NumberOfShareOptionsHeld_23daf2e4-b8b4-4e2c-b457-a9a7db4c794c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfOutstandingShareOptions_2a5a506c-4b9e-4da4-99ae-33be37f540ef" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NumberOfOutstandingShareOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_b78d50c7-ae4d-482b-8103-bf597c15606c" xlink:to="loc_ifrs-full_NumberOfOutstandingShareOptions_2a5a506c-4b9e-4da4-99ae-33be37f540ef" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PurchasesOfPropertyAndOtherAssetsRelatedPartyTransactions_83dffd03-ccd0-4822-8b75-a7c981f70876" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_PurchasesOfPropertyAndOtherAssetsRelatedPartyTransactions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_b78d50c7-ae4d-482b-8103-bf597c15606c" xlink:to="loc_ifrs-full_PurchasesOfPropertyAndOtherAssetsRelatedPartyTransactions_83dffd03-ccd0-4822-8b75-a7c981f70876" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProvisionsForDoubtfulDebtsRelatedToOutstandingBalancesOfRelatedPartyTransaction_6cb8ee89-e307-4f9e-a6ea-9755a0274c39" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ProvisionsForDoubtfulDebtsRelatedToOutstandingBalancesOfRelatedPartyTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_b78d50c7-ae4d-482b-8103-bf597c15606c" xlink:to="loc_ifrs-full_ProvisionsForDoubtfulDebtsRelatedToOutstandingBalancesOfRelatedPartyTransaction_6cb8ee89-e307-4f9e-a6ea-9755a0274c39" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ExpenseRecognisedDuringPeriodForBadAndDoubtfulDebtsForRelatedPartyTransaction_d73aa9ef-3480-41d1-a758-2d0d326d1366" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_ExpenseRecognisedDuringPeriodForBadAndDoubtfulDebtsForRelatedPartyTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_b78d50c7-ae4d-482b-8103-bf597c15606c" xlink:to="loc_ifrs-full_ExpenseRecognisedDuringPeriodForBadAndDoubtfulDebtsForRelatedPartyTransaction_d73aa9ef-3480-41d1-a758-2d0d326d1366" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/RelatedPartyDisclosuresSummaryofTransactionsBetweenRelatedPartiesAmountDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#RelatedPartyDisclosuresSummaryofTransactionsBetweenRelatedPartiesAmountDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/RelatedPartyDisclosuresSummaryofTransactionsBetweenRelatedPartiesAmountDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_b3aba881-275e-40e3-9af8-85fb04170157" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_842e0306-5f4f-4220-b607-7b7229ad55d8" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_b3aba881-275e-40e3-9af8-85fb04170157" xlink:to="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_842e0306-5f4f-4220-b607-7b7229ad55d8" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_08e9fe11-8193-44ea-b168-46341ac6c745" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_842e0306-5f4f-4220-b607-7b7229ad55d8" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_08e9fe11-8193-44ea-b168-46341ac6c745" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_08e9fe11-8193-44ea-b168-46341ac6c745_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_08e9fe11-8193-44ea-b168-46341ac6c745" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_08e9fe11-8193-44ea-b168-46341ac6c745_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_65eb8289-8f15-49ef-a3ca-922af83458c7" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_08e9fe11-8193-44ea-b168-46341ac6c745" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_65eb8289-8f15-49ef-a3ca-922af83458c7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherRelatedPartiesMember_525431ae-09f9-4048-90e5-aa677ea27144" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_OtherRelatedPartiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_65eb8289-8f15-49ef-a3ca-922af83458c7" xlink:to="loc_ifrs-full_OtherRelatedPartiesMember_525431ae-09f9-4048-90e5-aa677ea27144" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PurchasesOfGoodsAndServiceRelatedPartyTransactions_dce50d9a-029c-4c27-b724-b545a02fe629" xlink:href="bntx-20241231.xsd#bntx_PurchasesOfGoodsAndServiceRelatedPartyTransactions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_b3aba881-275e-40e3-9af8-85fb04170157" xlink:to="loc_bntx_PurchasesOfGoodsAndServiceRelatedPartyTransactions_dce50d9a-029c-4c27-b724-b545a02fe629" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PurchasesOfPropertyAndOtherAssetsRelatedPartyTransactions_94e14a30-b2e6-4f6c-bed6-89ff5ea4733f" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_PurchasesOfPropertyAndOtherAssetsRelatedPartyTransactions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_b3aba881-275e-40e3-9af8-85fb04170157" xlink:to="loc_ifrs-full_PurchasesOfPropertyAndOtherAssetsRelatedPartyTransactions_94e14a30-b2e6-4f6c-bed6-89ff5ea4733f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PurchasesFromRelatedPartyTransactions_01b1c067-f84b-4975-9cf1-d0576d1ba444" xlink:href="bntx-20241231.xsd#bntx_PurchasesFromRelatedPartyTransactions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_b3aba881-275e-40e3-9af8-85fb04170157" xlink:to="loc_bntx_PurchasesFromRelatedPartyTransactions_01b1c067-f84b-4975-9cf1-d0576d1ba444" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/RelatedPartyDisclosuresSummaryofTransactionsBetweenRelatedPartiesOutstandingBalancesDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#RelatedPartyDisclosuresSummaryofTransactionsBetweenRelatedPartiesOutstandingBalancesDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/RelatedPartyDisclosuresSummaryofTransactionsBetweenRelatedPartiesOutstandingBalancesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_2f8d0164-f628-4e0c-8121-9565e329db4e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_ff12a628-8127-483a-aa7d-d130040f76e3" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_2f8d0164-f628-4e0c-8121-9565e329db4e" xlink:to="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_ff12a628-8127-483a-aa7d-d130040f76e3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_8a4e09c8-c3ac-443a-a402-05e773033e41" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_ff12a628-8127-483a-aa7d-d130040f76e3" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_8a4e09c8-c3ac-443a-a402-05e773033e41" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_8a4e09c8-c3ac-443a-a402-05e773033e41_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_8a4e09c8-c3ac-443a-a402-05e773033e41" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_8a4e09c8-c3ac-443a-a402-05e773033e41_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_2cc73f95-5c76-4e83-b421-98f22e3ead63" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_8a4e09c8-c3ac-443a-a402-05e773033e41" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_2cc73f95-5c76-4e83-b421-98f22e3ead63" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherRelatedPartiesMember_c2d9326d-f5e3-41f7-ab8c-af847b183dc1" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_OtherRelatedPartiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_2cc73f95-5c76-4e83-b421-98f22e3ead63" xlink:to="loc_ifrs-full_OtherRelatedPartiesMember_c2d9326d-f5e3-41f7-ab8c-af847b183dc1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_1fa2e622-4535-44c2-8e3f-845a88b2a616" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesTable_ff12a628-8127-483a-aa7d-d130040f76e3" xlink:to="loc_srt_CounterpartyNameAxis_1fa2e622-4535-44c2-8e3f-845a88b2a616" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_1fa2e622-4535-44c2-8e3f-845a88b2a616_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_1fa2e622-4535-44c2-8e3f-845a88b2a616" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_1fa2e622-4535-44c2-8e3f-845a88b2a616_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_9b54596f-9f2d-484d-af59-0f829097e4b1" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_1fa2e622-4535-44c2-8e3f-845a88b2a616" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_9b54596f-9f2d-484d-af59-0f829097e4b1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_ATHOSKGMember_7cb97047-bd46-4954-b129-7dc2e4327750" xlink:href="bntx-20241231.xsd#bntx_ATHOSKGMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_9b54596f-9f2d-484d-af59-0f829097e4b1" xlink:to="loc_bntx_ATHOSKGMember_7cb97047-bd46-4954-b129-7dc2e4327750" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AmountsPayableRelatedPartyTransactions_6388d393-6350-4793-a408-213cf0f6961d" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_AmountsPayableRelatedPartyTransactions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_2f8d0164-f628-4e0c-8121-9565e329db4e" xlink:to="loc_ifrs-full_AmountsPayableRelatedPartyTransactions_6388d393-6350-4793-a408-213cf0f6961d" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.biontech.de/role/EventsAftertheReportingPeriodAdditionalInformationDetails" xlink:type="simple" xlink:href="bntx-20241231.xsd#EventsAftertheReportingPeriodAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.biontech.de/role/EventsAftertheReportingPeriodAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfNonadjustingEventsAfterReportingPeriodLineItems_d5ec363f-6408-424f-96a0-e8b8e23aac6e" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfNonadjustingEventsAfterReportingPeriodLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfNonadjustingEventsAfterReportingPeriodTable_ecd165c0-f9b2-4c6f-ada2-0748efa631ec" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_DisclosureOfNonadjustingEventsAfterReportingPeriodTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfNonadjustingEventsAfterReportingPeriodLineItems_d5ec363f-6408-424f-96a0-e8b8e23aac6e" xlink:to="loc_ifrs-full_DisclosureOfNonadjustingEventsAfterReportingPeriodTable_ecd165c0-f9b2-4c6f-ada2-0748efa631ec" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_427d24bd-5e3b-4bcb-81f9-3487082151ed" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfNonadjustingEventsAfterReportingPeriodTable_ecd165c0-f9b2-4c6f-ada2-0748efa631ec" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_427d24bd-5e3b-4bcb-81f9-3487082151ed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_427d24bd-5e3b-4bcb-81f9-3487082151ed_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_427d24bd-5e3b-4bcb-81f9-3487082151ed" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_427d24bd-5e3b-4bcb-81f9-3487082151ed_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_b1f53608-70db-4fe2-a9a4-a087eba49a68" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_427d24bd-5e3b-4bcb-81f9-3487082151ed" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_b1f53608-70db-4fe2-a9a4-a087eba49a68" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_LicenseAndOptionAgreementMember_ddbf79cd-5967-4bb9-80ce-31b1147880d2" xlink:href="bntx-20241231.xsd#bntx_LicenseAndOptionAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_b1f53608-70db-4fe2-a9a4-a087eba49a68" xlink:to="loc_bntx_LicenseAndOptionAgreementMember_ddbf79cd-5967-4bb9-80ce-31b1147880d2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_PurchaseOfSharesUnderAgreement_b7333f3c-b944-4f8f-86ba-3ac942111e57" xlink:href="bntx-20241231.xsd#bntx_PurchaseOfSharesUnderAgreement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfNonadjustingEventsAfterReportingPeriodLineItems_d5ec363f-6408-424f-96a0-e8b8e23aac6e" xlink:to="loc_bntx_PurchaseOfSharesUnderAgreement_b7333f3c-b944-4f8f-86ba-3ac942111e57" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PercentageOfVotingEquityInterestsAcquired_8ddc328f-bc52-4fa6-a4e6-833db3ed34f9" xlink:href="https://xbrl.ifrs.org/taxonomy/2024-03-27/full_ifrs/full_ifrs-cor_2024-03-27.xsd#ifrs-full_PercentageOfVotingEquityInterestsAcquired"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfNonadjustingEventsAfterReportingPeriodLineItems_d5ec363f-6408-424f-96a0-e8b8e23aac6e" xlink:to="loc_ifrs-full_PercentageOfVotingEquityInterestsAcquired_8ddc328f-bc52-4fa6-a4e6-833db3ed34f9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_bntx_UpfrontPayment_b65ac9fd-cb1e-4016-8f73-4844ee128300" xlink:href="bntx-20241231.xsd#bntx_UpfrontPayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfNonadjustingEventsAfterReportingPeriodLineItems_d5ec363f-6408-424f-96a0-e8b8e23aac6e" xlink:to="loc_bntx_UpfrontPayment_b65ac9fd-cb1e-4016-8f73-4844ee128300" xlink:type="arc" order="2"/>
  </link:definitionLink>
</link:linkbase>
